BBa_E2050 1 mRFP1 derivative of mRFP1, yeast-optimized 2005-01-24T12:00:00Z 2016-01-25T02:35:54Z Shaner et al, 2004. Nat Biotech (22):1567-1571 Released HQ 2013 mRFP derivative. Ex548nm/Em562. yeast codon optimized. false false _8_ 4206 230 7 In stock false Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mCherry (BBa_E2060). true ryu annotation1431920 1 mOrange range1431920 1 1 738 annotation1431915 1 MATSG linker range1431915 1 1 15 annotation1431919 1 GSGTA linker range1431919 1 724 738 annotation2214024 1 Help:Barcodes range2214024 1 745 769 BBa_M36529 1 BBa_M36529 In presence of lactose, produces mCherry 2015-10-21T11:00:00Z 2015-12-03T07:26:17Z mCherry, Ptet In previous part, creation of lacI protein represses the expression of mCherry. This occurs in the presence of lactose, and another part used in tandem controls the expression levels of mCherry by varying the expression of LacI. true false _848_ 29107 29107 9 false Ptet specialized to fit yeast false Valerie Wesley Scott component2478348 1 BBa_E2050 component2478343 1 BBa_M36527 component2478342 1 BBa_M36526 annotation2478348 1 BBa_E2050 range2478348 1 65 833 annotation2478343 1 BBa_M36527 range2478343 1 21 58 annotation2478342 1 BBa_M36526 range2478342 1 1 12 BBa_M36527 1 BBa_M36527 Lactose inducible yeast promoter 2015-10-22T11:00:00Z 2016-02-10T02:35:24Z Was extracted from the BBa_K1164004 part on iGem's Registry of Standard Biological Parts. In the presence of LacI, this sequence represses gene expression. Expression can be induced by the addition of lactose or IPTG, which binds to LacI, removing it from the DNA. false false _848_ 4206 29107 9 false Had to ensure that it would work in yeast and where to put this with regards to the Kozak sequence and protein coding region. Ended up being placed directly before the Kozak sequence. false Valerie Wesley Scott BBa_M36526 1 BBa_M36526 Kozak 2015-10-22T11:00:00Z 2015-12-03T07:22:38Z Was from part BBa_K792012 on igem's Registry of Standard Biological Parts A kozak sequence for yeast. Is missing the last three base pairs (ATG) which should be the first three of your protein coding section. Should be placed directly before this protein coding section true false _848_ 29107 29107 9 false Wanted to ensure that it would work in yeast and several other igem projects said it was used successfully. false Valerie Wesley Scott BBa_E2050_sequence 1 atggcaactagcggcatggttagtaaaggagaagaaaacaatatggcaatcataaaagaatttatgagattcaaagtcagaatggaaggttctgtaaatggtcacgagttcgaaatagaaggggaaggtgaaggtagaccctatgaaggctttcaaacggctaaattaaaagttaccaagggtggaccattgccctttgcttgggatatcctgtctcctcagttcacttatggtagtaaagcctatgttaagcatcctgctgatattcctgattacttcaagttgagttttccagaaggtttcaaatgggagagagttatgaattttgaagatggcggagttgtgacagtgacacaagactcctcacttcaagacggtgagtttatttacaaggtaaaactacgtggcactaactttccgtcggatggaccagtcatgcaaaaaaagacgatgggttgggaggcttcatctgagcgaatgtatccagaagatggggcactaaagggcgaaattaagatgaggctcaaattaaaggatggtggacattatacctcggaagtgaaaactacctataaagccaaaaagccagttcaattacctggtgcatacattgttggcattaagttggacatcacaagccacaatgaagattatacaatagtagagcagtacgaacgcgcggaaggtaggcattctactggaggcatggatgaactatacaaaggttctggtaccgcataataactctgatagtgctagtgtagatctc BBa_M36529_sequence 1 cccgccgccacctactagagtgttgtgtggaattgtgagcggataacaatttcacacatactagatggcaactagcggcatggttagtaaaggagaagaaaacaatatggcaatcataaaagaatttatgagattcaaagtcagaatggaaggttctgtaaatggtcacgagttcgaaatagaaggggaaggtgaaggtagaccctatgaaggctttcaaacggctaaattaaaagttaccaagggtggaccattgccctttgcttgggatatcctgtctcctcagttcacttatggtagtaaagcctatgttaagcatcctgctgatattcctgattacttcaagttgagttttccagaaggtttcaaatgggagagagttatgaattttgaagatggcggagttgtgacagtgacacaagactcctcacttcaagacggtgagtttatttacaaggtaaaactacgtggcactaactttccgtcggatggaccagtcatgcaaaaaaagacgatgggttgggaggcttcatctgagcgaatgtatccagaagatggggcactaaagggcgaaattaagatgaggctcaaattaaaggatggtggacattatacctcggaagtgaaaactacctataaagccaaaaagccagttcaattacctggtgcatacattgttggcattaagttggacatcacaagccacaatgaagattatacaatagtagagcagtacgaacgcgcggaaggtaggcattctactggaggcatggatgaactatacaaaggttctggtaccgcataataactctgatagtgctagtgtagatctc BBa_M36526_sequence 1 cccgccgccacc BBa_M36527_sequence 1 cctctcctttttgaaatattatgcagaggttgcgcggcgtgcactgggcgcggggcaggtataaaggtgggtggtttggggtgtgtggaattgtgagcggataacaatttcacacaacgtagggggcgaacggcgagattaaaagtatcaacaaaaaatgatggtggttggtttgtgtggaaagaaatgcgagccatgtttccggatctatccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z