BBa_M36771 1 BBa_M36771 Strong RBS from DNA 2.0 Gene Designer 2014-10-22T11:00:00Z 2015-05-08T01:14:06Z DNA 2.0 Gene Designer Strong Ribosome Binding Site from DNA 2.0 Gene Designer. false false _848_ 0 24189 9 Not in stock false None. false Preston Lim BBa_M36933 1 BBa_M36933 P_rhaBAD 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 A strong promoter. false false _848_ 0 24151 9 Not in stock false None false Emily Kelly BBa_M36551 1 BBa_M36551 OYE6 Protein Sequence 2015-10-21T11:00:00Z 2015-12-07T03:51:56Z This part comes directly from GenBank (http://www.ncbi.nlm.nih.gov/nuccore/JQ765594.1) except has its final codon (i.e., the stop codon) cut out. Note that this enables the sequence to be used using a FLAG/HIS tag afterwards. Final Codon (i.e., Stop Codon): Removed Codes for the Old Yellow Enzyme 6 (OYE6) from Achromobacter sp. OYE6 is part of the Old Yellow Enzyme (OYE) family, all of which are thought to function in reducing alpha-beta unsaturated C=C double bonds. In our experiment, we are using OYE6 to reduce citral to citronellal (by reducing an alpha-beta unsaturated C=C double bond in citral). OYE6 can be used by simply coding for the enzyme in a DNA sequence. As a result, the enzyme should be produced and can be used to reduce certain compounds that need to be introduced separately in vitro or in vivo. false false _848_ 29091 29091 9 false Again, this came directly from GenBank (http://www.ncbi.nlm.nih.gov/nuccore/JQ765594.1) with the caveat of having its final codon (i.e., its stop codon) cut out. false Aprotim Cory Bhowmik, Cali Nguyen, Zoe Lee-Chiong BBa_M36935 1 BBa_M36935 Term_PhageT7 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 Terminator recommended by DNA 2.0. false false _848_ 0 24151 9 Not in stock false None. false Emily Kelly BBa_M36084 1 BBa_M36084 Histidine Tag and Stop Codon 2014-04-29T11:00:00Z 2015-05-08T01:14:02Z We know that histidine tags are a sequence of 6 Histidines and 1 stop codon Histidine Tag and Stop Codon Sequences false false _848_ 0 22183 9 Not in stock false Histidine tag must be before stop codon false Ashley Hammerbacher Celina Malav?? BBa_M36553 1 BBa_M36553 OYE6 Composite Sequence 2015-10-21T11:00:00Z 2015-12-07T03:51:40Z All of these are basic parts that have each of their sources documented individually. Consists of multiple parts to produce the Old Yellow Enzyme 6 (OYE6) to reduce alpha-beta unsaturated C=C double bonds. Specifically consists of a rhamnose-inducible promoter (P_rha_BAD), a strong RBS, the OYE6 coding sequence, a His_* tag, and a termination sequence from T7. false false _848_ 29091 29091 9 false This design was done using a DNA2.0 software and GeneDesigner2.0 quality checks. The recommended design (in terms of promoters, RBS, tags, and terminators) was taken without amendments. false Aprotim Cory Bhowmik, Cali Nguyen, Zoe Lee-Chiong component2478233 1 BBa_M36771 component2478234 1 BBa_M36551 component2478232 1 BBa_M36933 component2478235 1 BBa_M36084 component2478236 1 BBa_M36935 annotation2478233 1 BBa_M36771 range2478233 1 115 127 annotation2478234 1 BBa_M36551 range2478234 1 128 1276 annotation2478232 1 BBa_M36933 range2478232 1 1 114 annotation2478235 1 BBa_M36084 range2478235 1 1277 1297 annotation2478236 1 BBa_M36935 range2478236 1 1298 1388 BBa_M36935_sequence 1 accgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccgggtaac BBa_M36553_sequence 1 caccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaattcaggcgctttttagactggaggaggtaaaaaaatgaacacgttcaccggtcgcctggaaaacgacgggcgcgcaacgcccgccaacctgttcgcgccgctgcgggtgggaaaacttcacctgcccaaccgcctggccgtcgcgcccatgacccgggtcagcgccacggccgacggccgcgccaccgggcagatggccgactactacgaggcctttgccgccggcggcttcggcctggtaatcaccgaaggcctctatacggacaaggctcatgcccagggctaccttttccagcccggcctggccgacgacgcgcagcgcgatgcgtggcggccggtggtggagcgggtgcatgcgcagggcgggcgcatcgtcgcgcagctcatgcatgcgggcgcgctgtcgcaaggcaatccgcatcgtcccacgacgaaagggccctcggccgtgttgccggcgggccagcagatggccttctatcggggccaagggcaataccggatgccggaggcgatgacggcccatgagatccgcgccgcggtcgaaggctttgcgcaggccgcgcgccgggcgcagcaggcgggattcgatggcgtcgagatacacggcgccaacggctatctgctggaccagttcctcacggcgcacaccaatctgcgcgatgaccgctacggcggcagcctggacaaccgcctgcgcctgacggtggaggtcatccaggcggtccggcagtcggcgtcggcgggctttgtcgtgggcgtgcgcagttcgcaaggcaaggtcaacgacttcacccaccagtggccgggcggccaggacgatgcggcccgcatcttttgcacgctgggcgcgcaggccatcgattacctgcacacgaccgagttcgaggcctggcgccccgccttcggcgaaagcggccccagcctggcggcgctggcgcgcacgcatgcctcggtgccggtgctggccaacggttcgctgcatgacgcggcgcgggccgaaggcgtgctgtcgcggcaggaggcggacttcatttcgctgggccggggcgcgctcacgcacgccgactggccggcaaggctgcgtgcaggcatgccgcacgaggaattcgaccgggggctgctcgcgccgatcgcggacctcgacaatgcggcccgccatcgcgcgcaggttcagctggcgcatcatcatcatcatcattagaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccgggtaac BBa_M36084_sequence 1 catcatcatcatcatcattag BBa_M36551_sequence 1 atgaacacgttcaccggtcgcctggaaaacgacgggcgcgcaacgcccgccaacctgttcgcgccgctgcgggtgggaaaacttcacctgcccaaccgcctggccgtcgcgcccatgacccgggtcagcgccacggccgacggccgcgccaccgggcagatggccgactactacgaggcctttgccgccggcggcttcggcctggtaatcaccgaaggcctctatacggacaaggctcatgcccagggctaccttttccagcccggcctggccgacgacgcgcagcgcgatgcgtggcggccggtggtggagcgggtgcatgcgcagggcgggcgcatcgtcgcgcagctcatgcatgcgggcgcgctgtcgcaaggcaatccgcatcgtcccacgacgaaagggccctcggccgtgttgccggcgggccagcagatggccttctatcggggccaagggcaataccggatgccggaggcgatgacggcccatgagatccgcgccgcggtcgaaggctttgcgcaggccgcgcgccgggcgcagcaggcgggattcgatggcgtcgagatacacggcgccaacggctatctgctggaccagttcctcacggcgcacaccaatctgcgcgatgaccgctacggcggcagcctggacaaccgcctgcgcctgacggtggaggtcatccaggcggtccggcagtcggcgtcggcgggctttgtcgtgggcgtgcgcagttcgcaaggcaaggtcaacgacttcacccaccagtggccgggcggccaggacgatgcggcccgcatcttttgcacgctgggcgcgcaggccatcgattacctgcacacgaccgagttcgaggcctggcgccccgccttcggcgaaagcggccccagcctggcggcgctggcgcgcacgcatgcctcggtgccggtgctggccaacggttcgctgcatgacgcggcgcgggccgaaggcgtgctgtcgcggcaggaggcggacttcatttcgctgggccggggcgcgctcacgcacgccgactggccggcaaggctgcgtgcaggcatgccgcacgaggaattcgaccgggggctgctcgcgccgatcgcggacctcgacaatgcggcccgccatcgcgcgcaggttcagctggcg BBa_M36933_sequence 1 caccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaattcaggcgctttttagactgg BBa_M36771_sequence 1 aggaggtaaaaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z