BBa_M36724 1 BBa_M36724 5' Bicistronic UTR (strong), does not include ATG start 2011-04-27T11:00:00Z 2015-05-08T01:14:06Z Vivek Mutalik et al. c/o BIOFAB Emeryville. 5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information. false false _848_ 0 52 432 Not in stock false Vivek says, "This is (the) Makoff design, it gives good expression and is seq independent of the Schoner design." false Drew Endy BBa_M36655 1 BBa_M36655 C-24(28) sterol reductase 2012-12-04T12:00:00Z 2015-05-08T01:14:05Z This sequence is derived from Schizosaccharomyces pombe 972h- (strain: 972h-). Gene ID :2541740 in the NCBI database. Enzyme coding sequence for C-24(28) sterol reductase, an enzyme which acts in the last step of Vitamin D2 synthesis by reducing ergosta-5,7,22,24(28)-tetraenol to ergosterol. No mutations were made to the enzymes genetic sequence. The genetic sequence is optimized for use in Synechococcus elongatus (PCC 7942). false false _848_ 0 15023 9 Not in stock false The coding sequence denoted by Gene ID :2541740 has to be codon optimized for Synechococcus elongatus. false Elvis Ikwa, Alexis Garduno, Johnathan Bowes annotation2213461 1 Stop range2213461 1 1360 1362 annotation2213462 1 C-24(28) sterol reductase coding region range2213462 1 1 1362 annotation2213460 1 start range2213460 1 1 3 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_M36557 1 BBa_M36557 VItamin D2 Actuator 2012-12-04T12:00:00Z 2015-05-08T01:14:05Z This part was inspired from the following paper ( Tomoko Ikwaki,Haruyuki lefuji,Yoshikazu Hiraga,Akira Hosomi, Tomotake Morita, Yuko GigaHamam Kaoru Takegawa.???Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe???.7 December 2007) a direct link is available below http://mic.sgmjournals.org/content/154/3/830.full This device converts ergosta-5,7,22,24(28)-tetraen-3&#946;-ol to ergosterol. In the presence of UV light ergosterol is converted to Vitamin D2. The coding region[BBa_M36655] produces C-24(28) sterol reductase an enzyme that catalyzes the final step in Vitamin D2 synthesis pathway by converting ergosta-5,7,22,24(28)-tetraen-3&#946;-ol to ergosterol. false true _848_ 0 15023 9 Not in stock false This construct is uses a strong RBS and 5'UTR so that considerable amounts of C-24(28) sterol reductase to convert the precursor to ergosterol. false Elvis Ikwa,Alexis Garduno, Johnathan Bowes component2213624 1 BBa_M36724 component2213635 1 BBa_B0015 component2213628 1 BBa_M36655 annotation2213628 1 BBa_M36655 range2213628 1 92 1453 annotation2213624 1 BBa_M36724 range2213624 1 1 85 annotation2213635 1 BBa_B0015 range2213635 1 1462 1590 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M36557_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctgtactagatgaaatctacagtaaagaaatcagctcctcgtgagtttggcggtgccaaaggtgcattggcaattatgacaggattcccttgtctaatgtattacttatgggcatgctccaaatttaatgattcgcagtttattaaaccagaatcttttacgatagcaggatttcaaaatttttttagaacgttaggccattatatctacgtcggtgcctatccaacacggtatgcattcttggttttttggtcgttttgtatagttcaggctgtcatgtacttaactcttcctggtgttcggacgcaaggtttgcctttaaagcatcgcaataacgaacgtcttccttacttatgcaatgctatctggtctttttacacgacaattgttattcttgccgttcttcacgtgacccacgtatttccaattactacatttatcgacatgttcggtcctttgatgagcgttgcaataataaccgcttttgtctgtacctttgttttgtacactggaactttgctttttggtgatcgactttttgataaacctcatcggttatctggaaatcccatctacgatgcctttatgggagcttgcttgaatcctcgccttggcaaattgcttgattttaaaatgttctttgaagttcgtatcccttggtttatcctattctttatttccgttggtgctgctgttcgtcaatatgaaacttatggcactgtaagtcctcaggtacttttcgtttgtcttggccattatttgtatgcaaacgcgtgctcgaagggagagcagttgattgttccaacttgggacatggcttatgaaaagtttggttttatgcttatcttttggaacatggctggggtcccatttacttattcacactgcaccctttaccttttttcgcatgatccatctgtttacaactggtcaactcaatatacaaccggtatttatgttttgcttctttgctgttattatatatttgatacttgcaatggtcaaaaaaatcatttccgtaatcaaatttatggtactgaggttcatcgcaaaacatttccccaacttccctggcttatcatcaagaaccctacctttattcgttgtgcaaatggcggtactttgttgacttcaggttggtataggtacgctcgtaaaattcattatactgctgatttctttcaatcattgtcatgggcattgatcaccggttttcagtcccctctaccttacttttacccatgctttttctttgtagtccttgtgcatcgtgtttcacgtgatattaaaaagtgcaaagctaagtatggggctgatttcgatgagtattgccgtatatgcccttacttatttattccctatatattttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M36655_sequence 1 atgaaatctacagtaaagaaatcagctcctcgtgagtttggcggtgccaaaggtgcattggcaattatgacaggattcccttgtctaatgtattacttatgggcatgctccaaatttaatgattcgcagtttattaaaccagaatcttttacgatagcaggatttcaaaatttttttagaacgttaggccattatatctacgtcggtgcctatccaacacggtatgcattcttggttttttggtcgttttgtatagttcaggctgtcatgtacttaactcttcctggtgttcggacgcaaggtttgcctttaaagcatcgcaataacgaacgtcttccttacttatgcaatgctatctggtctttttacacgacaattgttattcttgccgttcttcacgtgacccacgtatttccaattactacatttatcgacatgttcggtcctttgatgagcgttgcaataataaccgcttttgtctgtacctttgttttgtacactggaactttgctttttggtgatcgactttttgataaacctcatcggttatctggaaatcccatctacgatgcctttatgggagcttgcttgaatcctcgccttggcaaattgcttgattttaaaatgttctttgaagttcgtatcccttggtttatcctattctttatttccgttggtgctgctgttcgtcaatatgaaacttatggcactgtaagtcctcaggtacttttcgtttgtcttggccattatttgtatgcaaacgcgtgctcgaagggagagcagttgattgttccaacttgggacatggcttatgaaaagtttggttttatgcttatcttttggaacatggctggggtcccatttacttattcacactgcaccctttaccttttttcgcatgatccatctgtttacaactggtcaactcaatatacaaccggtatttatgttttgcttctttgctgttattatatatttgatacttgcaatggtcaaaaaaatcatttccgtaatcaaatttatggtactgaggttcatcgcaaaacatttccccaacttccctggcttatcatcaagaaccctacctttattcgttgtgcaaatggcggtactttgttgacttcaggttggtataggtacgctcgtaaaattcattatactgctgatttctttcaatcattgtcatgggcattgatcaccggttttcagtcccctctaccttacttttacccatgctttttctttgtagtccttgtgcatcgtgtttcacgtgatattaaaaagtgcaaagctaagtatggggctgatttcgatgagtattgccgtatatgcccttacttatttattccctatatattttag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M36724_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctg BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z