BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_M36576
1
BBa_M36576
qsc102 promoter + Whiteley Spacing
2015-10-21T11:00:00Z
2015-10-22T10:25:01Z
qsc102 promoter: Boxed region from Figure 1 of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/
Whiteley spacing: Region following boxed region to bolded A of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/
Palindromic binding sequence and spacing for LasR bound to Pseudomonas Autoinducer.
false
false
_848_
29087
29087
9
false
Added 11 preceding base pairs to qsc102 palindromic binding sequence in order to optimize LasR complex binding.
false
Elisa Vidales
annotation2478317
1
Binding Spacing 1
range2478317
1
1
11
annotation2478312
1
LasR binding sequence
range2478312
1
12
31
annotation2478319
1
Whiteley Spacing
range2478319
1
32
64
annotation2478320
1
Transcription Start Site
range2478320
1
65
65
BBa_M36580
1
BBa_M36580
LasR/PAI complex sensor
2015-10-21T11:00:00Z
2015-10-23T06:17:49Z
qsc102 promoter: Boxed region from Figure 1 of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/
Whiteley spacing: Region following boxed region to bolded A of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/
BBa_0030 strong RBS: partsregistry.org
GFP coding sequence: BIOE44 pComet DNA sequence
Sensor for complex made up of LasR bound to Pseudomonas Autoinducer. Contains qsc102 promoter, BBa_0030 strong RBS, GFP coding sequence.
false
false
_848_
29087
29087
9
false
Added preceding spacing to qsc102 promoter palindromic binding sequence, as well as spacing flanking the RBS in order to optimize binding.
false
Elisa Vidales
component2478302
1
BBa_M36576
component2478304
1
BBa_B0032
annotation2478304
1
BBa_B0032
range2478304
1
74
86
annotation2478302
1
BBa_M36576
range2478302
1
1
65
BBa_B0032_sequence
1
tcacacaggaaag
BBa_M36576_sequence
1
tgaatcactagacctgcccggaagggcaggtgtccctgccgggctgtgacaatttaattcgacca
BBa_M36580_sequence
1
tgaatcactagacctgcccggaagggcaggtgtccctgccgggctgtgacaatttaattcgaccatactagagtcacacaggaaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z