BBa_M36576 1 BBa_M36576 qsc102 promoter + Whiteley Spacing 2015-10-21T11:00:00Z 2015-10-22T10:25:01Z qsc102 promoter: Boxed region from Figure 1 of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ Whiteley spacing: Region following boxed region to bolded A of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ Palindromic binding sequence and spacing for LasR bound to Pseudomonas Autoinducer. false false _848_ 29087 29087 9 false Added 11 preceding base pairs to qsc102 palindromic binding sequence in order to optimize LasR complex binding. false Elisa Vidales annotation2478319 1 Whiteley Spacing range2478319 1 32 64 annotation2478317 1 Binding Spacing 1 range2478317 1 1 11 annotation2478312 1 LasR binding sequence range2478312 1 12 31 annotation2478320 1 Transcription Start Site range2478320 1 65 65 BBa_M36580 1 BBa_M36580 LasR/PAI complex sensor 2015-10-21T11:00:00Z 2015-10-23T06:17:49Z qsc102 promoter: Boxed region from Figure 1 of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ Whiteley spacing: Region following boxed region to bolded A of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ BBa_0030 strong RBS: partsregistry.org GFP coding sequence: BIOE44 pComet DNA sequence Sensor for complex made up of LasR bound to Pseudomonas Autoinducer. Contains qsc102 promoter, BBa_0030 strong RBS, GFP coding sequence. false false _848_ 29087 29087 9 false Added preceding spacing to qsc102 promoter palindromic binding sequence, as well as spacing flanking the RBS in order to optimize binding. false Elisa Vidales component2478302 1 BBa_M36576 component2478304 1 BBa_B0032 annotation2478302 1 BBa_M36576 range2478302 1 1 65 annotation2478304 1 BBa_B0032 range2478304 1 74 86 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation1709 1 RBS-3\Weak range1709 1 1 13 BBa_B0032_sequence 1 tcacacaggaaag BBa_M36576_sequence 1 tgaatcactagacctgcccggaagggcaggtgtccctgccgggctgtgacaatttaattcgacca BBa_M36580_sequence 1 tgaatcactagacctgcccggaagggcaggtgtccctgccgggctgtgacaatttaattcgaccatactagagtcacacaggaaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z