BBa_M36933 1 BBa_M36933 P_rhaBAD 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 A strong promoter. false false _848_ 0 24151 9 Not in stock false None false Emily Kelly BBa_M36586 1 BBa_M36586 T1 Transcription Terminator 2015-10-23T11:00:00Z 2015-10-24T07:19:18Z Sequence obtained from Genedesigner 2.0 A transcription terminator that works well with the ctfA, ctfB, and ADC genes. false false _848_ 29118 29118 9 false No design consideration encountered. false Aris Kare BBa_M36582 1 BBa_M36582 ctfB Gene Sequence 2015-10-23T11:00:00Z 2015-10-24T08:57:59Z Sequence for ctfB obtained from http://www.ncbi.nlm.nih.gov/gene/1116169 This part is the ctfB gene, or butyrate-acetoacetate COA-transferase subunit B, that is used in a complex mechanism/pathway to produce acetone. We are aiming to use this alone with several other enzymes to eventually produce acetone and CO2 from e.coli bacteria. This part, along with ctfA, produces acetoacetate and butyryl-CoA, which are the penultimate products before the final product, acetone. false false _848_ 29118 29118 9 false No design considerations encountered. false Aris Kare BBa_M36583 1 BBa_M36583 Strong RBS - BBa_J61100 2015-10-23T11:00:00Z 2015-10-24T07:12:57Z Genedesigner 2.0 Strong RBS obtained from Genedesigner 2.0 false false _848_ 29118 29118 9 false No design considerations considered. false Aris Kare BBa_M36584 1 BBa_M36584 Making acetoacetate from acetic acid and acetoacetyl-CoA 2015-10-23T11:00:00Z 2015-12-05T03:14:09Z Sequence for ctfA obtained from http://www.ncbi.nlm.nih.gov/gene/1116168 Sequence for ctfB obtained from http://www.ncbi.nlm.nih.gov/gene/1116169 This part begins with a promoter, the ctfA coding sequence, a strong RBS, the ctfB coding sequence, and a termination sequence from T1. false false _848_ 29084 29118 9 false Chose to use only one promoter for both ctfA and ctfB to shorten the length of the plasmid. false Aris Kare, Eda Benites, Ricky Cordova component2478371 1 BBa_M36583 component2478373 1 BBa_M36586 component2478370 1 BBa_M36581 component2478369 1 BBa_M36933 component2478372 1 BBa_M36582 annotation2478373 1 BBa_M36586 range2478373 1 1466 1544 annotation2478369 1 BBa_M36933 range2478369 1 1 114 annotation2478370 1 BBa_M36581 range2478370 1 115 771 annotation2478372 1 BBa_M36582 range2478372 1 800 1465 annotation2478371 1 BBa_M36583 range2478371 1 772 799 BBa_M36581 1 BBa_M36581 ctfA Gene Sequence 2015-10-23T11:00:00Z 2015-12-04T07:02:24Z Sequence for ctfA obtained from http://www.ncbi.nlm.nih.gov/gene/1116168 This part is the ctfA gene, or butyrate-acetoacetate COA-transferase subunit A, that is used in a complex mechanism/pathway to produce acetone. We are aiming to use this alone with several other enzymes to eventually produce acetone and CO2 from e.coli bacteria. This part, along with ctfB, produces acetoacetate and butyryl-CoA, which are the penultimate products before the final product, acetone. false false _848_ 29109 29118 9 false No design considerations encountered. false Aris Kare, Ricky Cordova, Eda Benites BBa_M36581_sequence 1 ttatgcaggctcctttactatataatttataagaactccgggggtcattgctttttccttttctagtttttcacagctaactaaattttcagcttcaactattacggttttagctgccattgccatatagggattaaagtttttagtagtacctttatagaaggtgtttccggcctcatctacaatactacctttaattaatgctacatcggctgtaagaggtagctctaacaaatattccgttccatttatagatatttttttctttcctttttcaatcaaagttcctaaacctgttttagttagtacaccacctaagccagatccgcctgcacgtattctttccactagagttccttggggagagagctctacttcaagttcattattaaaaagttttttgccagtatctgggttgctgcctatatatgaagcaataagcttttttacttgattatttgatattaacttaccaatacctgtattaggataacatgtatcattacttataatcgttaaattctttatatttaaattaactaaaaaatcaattaatttggttggagtgccacagtttaaaaaacctccaatcataattgtcatcccatctttaaagaatgaccttaaattttcaaatctaattattttagagttcat BBa_M36933_sequence 1 caccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaattcaggcgctttttagactgg BBa_M36586_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct BBa_M36583_sequence 1 tctagagaaagaggggacaaactagatg BBa_M36582_sequence 1 ctaaacagccatgggtctaagttcattggatatgagtaaatctgcagcagttaaagaccttatttcatcaatggttgtgtttttattaatttcagtgagaagtaaaccatcattaataacctcaattactccaagttctgttacaattagatttgcttgagactttgccgtgaggggaagtgtacatttttttaaaattttaggttgacctttatttgtatgtctcattgcaattattactttcttagctccatttactaaatccatagctccacccataccagagagcatttttccaggaacaatccaattggctatattacccttttcatctacctggagagcccctaaaacagtaacatctacgtgaccaccacggattagtgaaaacgaaactgagctatcgaaaaatgtgccgtcaggaagtactgttgtatagtctcctcctgcatttactacatctttatctgcctcatttattttaggactagcgcccattccaactattccgttttctgattggaaagtaattttgaaattttttggtatataatctgcaaccatggtaggaagacctacacctaagtttacaagttgaccattttttaattctcttgcaactcttttggctattatttctttcgctaggtttttatcattaatcat BBa_M36584_sequence 1 caccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaattcaggcgctttttagactggttatgcaggctcctttactatataatttataagaactccgggggtcattgctttttccttttctagtttttcacagctaactaaattttcagcttcaactattacggttttagctgccattgccatatagggattaaagtttttagtagtacctttatagaaggtgtttccggcctcatctacaatactacctttaattaatgctacatcggctgtaagaggtagctctaacaaatattccgttccatttatagatatttttttctttcctttttcaatcaaagttcctaaacctgttttagttagtacaccacctaagccagatccgcctgcacgtattctttccactagagttccttggggagagagctctacttcaagttcattattaaaaagttttttgccagtatctgggttgctgcctatatatgaagcaataagcttttttacttgattatttgatattaacttaccaatacctgtattaggataacatgtatcattacttataatcgttaaattctttatatttaaattaactaaaaaatcaattaatttggttggagtgccacagtttaaaaaacctccaatcataattgtcatcccatctttaaagaatgaccttaaattttcaaatctaattattttagagttcattctagagaaagaggggacaaactagatgctaaacagccatgggtctaagttcattggatatgagtaaatctgcagcagttaaagaccttatttcatcaatggttgtgtttttattaatttcagtgagaagtaaaccatcattaataacctcaattactccaagttctgttacaattagatttgcttgagactttgccgtgaggggaagtgtacatttttttaaaattttaggttgacctttatttgtatgtctcattgcaattattactttcttagctccatttactaaatccatagctccacccataccagagagcatttttccaggaacaatccaattggctatattacccttttcatctacctggagagcccctaaaacagtaacatctacgtgaccaccacggattagtgaaaacgaaactgagctatcgaaaaatgtgccgtcaggaagtactgttgtatagtctcctcctgcatttactacatctttatctgcctcatttattttaggactagcgcccattccaactattccgttttctgattggaaagtaattttgaaattttttggtatataatctgcaaccatggtaggaagacctacacctaagtttacaagttgaccattttttaattctcttgcaactcttttggctattatttctttcgctaggtttttatcattaatcatccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z