BBa_M36933 1 BBa_M36933 P_rhaBAD 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 A strong promoter. false false _848_ 0 24151 9 Not in stock false None false Emily Kelly BBa_M36586 1 BBa_M36586 T1 Transcription Terminator 2015-10-23T11:00:00Z 2015-10-24T07:19:18Z Sequence obtained from Genedesigner 2.0 A transcription terminator that works well with the ctfA, ctfB, and ADC genes. false false _848_ 29118 29118 9 false No design consideration encountered. false Aris Kare BBa_M36587 1 BBa_M36587 Making acetone from acetoacetate using ADC 2015-10-23T11:00:00Z 2015-10-24T07:34:08Z Sequence for ADC obtained from http://www.ncbi.nlm.nih.gov/gene/1116170 This part begins with a promoter, then the ADC coding sequence, and finally, a termination sequence from T1. This composite part aims to produce acetone from acetoacetate using the gene sequence of ADC. ADC, or acetoacetate decarboxylase, is able to convert the byproducts acetoacetate and butyryl-CoA (made by the gene sequences ctfA and ctfB) into acetate, the final product in this complex mechanism. This can be used in projects that are aiming to convert acetoacetate especially into acetone. false false _848_ 29118 29118 9 false No design considerations considered with this composite part specifically. false Aris Kare component2478376 1 BBa_M36586 component2478374 1 BBa_M36933 component2478375 1 BBa_M36585 annotation2478375 1 BBa_M36585 range2478375 1 115 849 annotation2478374 1 BBa_M36933 range2478374 1 1 114 annotation2478376 1 BBa_M36586 range2478376 1 850 928 BBa_M36585 1 BBa_M36585 ADC Gene Sequence 2015-10-23T11:00:00Z 2015-10-24T07:13:36Z Sequence for ctfB obtained from http://www.ncbi.nlm.nih.gov/gene/1116170 This part is the ADC gene, or acetoacetate decarboxylase, that is used in a complex mechanism/pathway to produce acetone. We are aiming to use this in our final step to produce acetone and CO2 from e.coli bacteria. This part makes the end result products, acetone and CO2, from acetoacetate. false false _848_ 29118 29118 9 false No design considerations encountered. false Aris Kare BBa_M36585_sequence 1 atgttaaaggatgaagtaattaaacaaattagcacgccattaacttcgcctgcatttcctagaggaccctataaatttcataatcgtgagtattttaacattgtatatcgtacagatatggatgcacttcgtaaagttgtgccagagcctttagaaattgatgagcccttagtcaggtttgaaattatggcaatgcatgatacgagtggacttggttgttatacagaaagcggacaggctattcccgtaagctttaatggagttaagggagattatcttcatatgatgtatttagataatgagcctgcaattgcagtaggaagggaattaagtgcatatcctaaaaagctcgggtatccaaagctttttgtggattcagatactttagtaggaactttagactatggaaaacttagagttgcgacagctacaatggggtacaaacataaagccttagatgctaatgaagcaaaggatcaaatttgtcgccctaattatatgttgaaaataatacccaattatgatggaagccctagaatatgtgagcttataaatgcgaaaatcacagatgttaccgtacatgaagcttggacaggaccaactcgactgcagttatttgatcacgctatggcgccacttaatgatttgccagtaaaagagattgtttctagctctcacattcttgcagatataatattgcctagagctgaagttatatatgattatcttaagtaa BBa_M36933_sequence 1 caccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaattcaggcgctttttagactgg BBa_M36586_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct BBa_M36587_sequence 1 caccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaattcaggcgctttttagactggatgttaaaggatgaagtaattaaacaaattagcacgccattaacttcgcctgcatttcctagaggaccctataaatttcataatcgtgagtattttaacattgtatatcgtacagatatggatgcacttcgtaaagttgtgccagagcctttagaaattgatgagcccttagtcaggtttgaaattatggcaatgcatgatacgagtggacttggttgttatacagaaagcggacaggctattcccgtaagctttaatggagttaagggagattatcttcatatgatgtatttagataatgagcctgcaattgcagtaggaagggaattaagtgcatatcctaaaaagctcgggtatccaaagctttttgtggattcagatactttagtaggaactttagactatggaaaacttagagttgcgacagctacaatggggtacaaacataaagccttagatgctaatgaagcaaaggatcaaatttgtcgccctaattatatgttgaaaataatacccaattatgatggaagccctagaatatgtgagcttataaatgcgaaaatcacagatgttaccgtacatgaagcttggacaggaccaactcgactgcagttatttgatcacgctatggcgccacttaatgatttgccagtaaaagagattgtttctagctctcacattcttgcagatataatattgcctagagctgaagttatatatgattatcttaagtaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z