BBa_M36596 1 HydA Oxygen-tolerant hydrogenase (with RBS and terminator) 2013-06-08T11:00:00Z 2015-05-08T01:14:05Z Bicistronic RBS: Mutalik VK, Guimaraes JC, Cambray G, Mai QA, Christoffersen MJ, Martin L, Yua A, Lam C, Rodriguez C, Bennett G, et al. Quantitative estimation of activity and quality for collections of functional genetic elements. Nat. Methods. 2013;10:347-353. BioFAB. (http://io.biofab.org:5000/simple/randomized_bds) Sequence for large subunit Hydrogenase: Nishihara H, Miyata Y, Miyashita Y, Bernhard M, Pohlmann A, et al. Analysis of the molecular species of hydrogenase in the cells of an obligately chemolithoautotrophic, marine hydrogen-oxidizing bacterium, Hydrogenovibrio marinus. Biosci Biotechnol Biochem. 2001;65:2780???2784. UnitPRO (http://www.uniprot.org/uniprot/F2Z6J6). High efficiency transcriptional terminator: Sunil Bodapati, Julia Joung, Daniel Esquivel-Reynoso. Part: BBa_M36281: Hard Information. Registry of Standard Biological Parts. (http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa%20M36281) Hydrogenase is an enzyme that catalyzes the reversible oxidation of H2. This part encodes a hydrogenase actuator taken from the large subunit of the marine bacterium Hydrogenovibrius Marinus, which has been shown to be relatively tolerant to oxygen (Kim et. Al, 2011). This part consists of a strong bicistronic design RBS (found under BioFAB ID: pFAB1655), a codon-optimized HmMBH actuator gene taken from the sequence of the large subunit of hydrogenase found in hydrogenovibrius marinus (original sequence found under F2Z6J6 at uniprot.org), and a high efficiency transcriptional terminator (found under BBa_M36281 in the Registry of Standard Biological Parts). The mechanism of the part is relatively straightforward: Once induced with the PoPS signal, the actuator will initiate protein translation and express the hydrogenase enzyme. Our actuator was used in conjunction with a rhamnose sensor (e.g. BBa_M36284). false false _848_ 0 17308 9 Not in stock false Considerations of the benefits and convenience of having a bicsistronic design led us to choose this option, in spite of the fact that the bicistronic design is targeted for E. Coli and has not yet been tested in S. Elongatus. Interestingly enough, although the part was originally designed for expression of the hydrogenase protein in cyanobacteria, it has so far only been proved for the expression of hydrogenase protein in E. Coli. In regards to the HmMBH actuator gene, the sequence was optimized to reduce repeats and the number of sites available to restriction enzymes. false Emma Marriott, Jessica Lam, Tatum Banayat annotation2329341 1 BBa_M36281 range2329341 1 1935 2016 annotation2329338 1 BBa_M36595 range2329338 1 1 87 annotation2329339 1 Hydrogenase Actuator range2329339 1 94 1926 annotation2329340 1 BBa_M36596 range2329340 1 94 1926 BBa_M36596_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgcgggagggtttctgattactagatgtccgttctgaatacgcccaaccactataagatggataacagtggacgccgcgttgtgatcgatccggtcacccgaatcgaagggcatatgcggtgcgaagtcaacgtggacgaaaacaacgtcatccagaacgctgtctcaacgggcacgatgtggcggggactcgaagtcatcctccgggggcgcgatccccgggatgcctgggcatttgtggagcgaatttgcggggtgtgtacaggctgtcacgccttagcaagcgtccgtgccgttgaggatgccttggacattaaaatcccgcacaacgcgacgctcattcgcgaaattatggctaaaacgctgcagatccacgatcatatcgttcatttttaccacctacacgctctggattgggtgaacccggtgaatgcccttaaagccgacccccaggcaacctcagaattacagaagctagtgtcacctcatcatccgatgtccagtcctggctacttcaaagatatccagatccgcatccagaagtttgtcgatagcggacaactaggcatttttaagaatgggtattggagcaatccagcttacaaactgagccccgaggccgatctcatggcagtcacacactacctcgaggccttggattttcagaaagagatcgtcaagatccacgctatctttggcggcaagaacccacatccgaactatatggtcggtggcgttccatgcgcgatcaacatcgatggcgatatggcggctggggcacccatcaacatggaacggctcaatttcgtcaagtcgttaattgaacagggccgtactttcaacactaacgtctatgtcccagatgtcatcgcgatcgccgcgttttaccgagattggctatacggggggggtttgtcagcaacaaatgtgatggattacggcgcatatccaaaaacaccttacgacaagtcgaccgaccaattgcccggtggcgctattatcaatggcgactggggtaaaatccaccccgtggatcctcgtgaccccgagcaggttcaagaatttgtgacgcacagctggtacaagtaccctgatgagacaaaaggactacatccatgggacggcattacggaaccgaattacgagcttggcagcaagactaagggctctcgtaccaacatcatcgagattgatgaatccgcaaagtacagctggatcaaatcgccccgttggcgcggacacgccgtcgaggtgggccctctggcccgctacattttggcctacgctcagggcgtggaatatgtcaagacccaggttcacacgtcgctgaaccgctttaatgcggtgtgcagactgctggatcccaatcacaaggatatcaccgatctcaaagccttcttgggaagtaccattggccgaactttagctcgcgctctggaaagtgagtattgcggcgatatgatgctggatgattttaatcagttaatcagcaacatcaagaatggtgatagctccactgcgaacaccgacaagtgggatccttcgagctggcctgagcatgctaaaggcgtaggcaccgtcgccgctccccgtggcgccttggcccactggattgtcattgagaaaggtaaaattaagaattaccagtgtgtggtgccgaccacctggaacggtagcccgcgcgaccctaaggggaatatcggtgccttcgaggcctctctgatgggcacccccatggaaagacccgatgagccggtagaagtcctgcgtactttgcactcctttgacccatgcttagcttgcagtacccacgtcatgagcgaggaaggcgaagagatggctacagtgaaggtgcgttactagagtcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z