BBa_M36598 1 BBa_M36598 Yeast-Optimized Chicken Lysozyme (LysC) Secretor 2014-10-23T11:00:00Z 2015-05-08T01:14:05Z The Lysozyme sequence is originally derived from Gallus Gallus. The Gal1 promoter and Cyc1 terminator are originally derived from Saccharomyces cerevisiae. Our device can be transformed into yeast for the production of mammalian lysozyme. It consists of a GAL1 promoter, the LysC gene linked to a yeast-optimized albumin signal peptide, and a Cyc1 terminator. Chicken lysozyme (LysC) has been shown to have high catalytic activity against gram-positive bacteria, hydrolyzing the peptidoglycan in the bacterial cell wall. The albumin signal peptide is optimized for lytic enzyme secretion in yeast expression hosts. By granting ethanologens, like yeast, the ability to secrete lysozyme, this part can be used to simultaneously decontaminate broths while carrying out fermentation. false false _848_ 0 24164 9 Not in stock false We checked to make sure the sequence did not include any BsaI restriction sites because we are cloning the gene into a vector using BsaI. Given that the signal peptide is derived from mammalian cells, it is uncertain whether or not the yeast will read the peptide and secrete the lysozyme properly from the cytosol, despite it being yeast optimized. false Daniel Hu, Andrew Austin Lee, Clinton Olivas component2429337 1 BBa_M36762 component2429338 1 BBa_K1462070 component2429332 1 BBa_J63006 annotation2429337 1 BBa_M36762 range2429337 1 556 999 annotation2429332 1 BBa_J63006 range2429332 1 1 549 annotation2429338 1 BBa_K1462070 range2429338 1 1008 1257 BBa_M36762 1 BBa_M36762 Chicken Lysozyme C (LYZ) conjugated with Albumin Signal Peptide 2014-10-22T11:00:00Z 2015-05-08T01:14:06Z The gene comes from iGem Part:BBa_K284001, a chicken lysozyme LysA gene. The signal peptide comes from Oxford Genetics optimized yeast lysozyme secretion vector, pSF-TEF1-NH2-Lys. Our part can be transformed into yeast for the production of mammalian lysozyme. Chicken lysozyme (LysA) has been shown to have high catalytic activity against gram-positive bacteria, hydrolyzing the peptidoglycan in the bacterial cell wall. The albumin signal peptide is optimized for lytic enzyme secretion in yeast expression hosts. By granting ethanologens, like yeast, the ability to secrete lysozyme, this part can be used to simultaneously decontaminate broths while carrying out fermentation. false false _848_ 0 24183 9 Not in stock false We checked to make sure the sequence did not include any BsaI restriction sites. Given that the signal peptide is derived from mammalian cells, it is uncertain whether or not the yeast will read the peptide and secrete the lysozyme properly from the cytosol, despite it being yeast optimized. false Andrew Lee, Clinton Olivas, Daniel Hu annotation2429015 1 Albumin signal peptide range2429015 1 1 55 annotation2429016 1 LYZ range2429016 1 56 444 annotation2429018 1 Stop range2429018 1 442 444 annotation2429017 1 Start range2429017 1 1 3 BBa_K1462070 1 BBa_K1462070 cyc1 2014-10-13T11:00:00Z 2015-05-08T01:10:34Z 1 1 false false _1841_ 0 21369 9 Not in stock false 1 false Chuyao Fan BBa_J63006 1 GAL1 yeast GAL1 promoter 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic GAL1 locus Released HQ 2013 GAL1 promoter from S. cerevisiae false true _97_ 0 545 97 In stock false unchanged from genomic sequence true Caroline Ajo-Franklin BBa_J63006_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag BBa_M36598_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatgaaatgggtgacctttatatctcttctctttttattcagctctgcttattcaaaggtatttgggaggtgtgagcttgccgctgctatgaagcgacacggattagacaactatcgaggatactccctcggtaattgggtttgtgctgcaaagtttgaaagtaactttaatacccaagccacgaatagaaatacagacgggtcaactgattacgggatccttcagataaatagcaggtggtggtgcaatgacggccgcactccaggatcacgtaatctttgcaacattccatgctcggcgttactttcgagtgacatcacagcgtcagtaaattgcgctaagaagatcgtgagtgatggcaatggcatgaacgcttgggtggcgtggcggaacaggtgtaaaggaacggacgttcaggcttggattagggggtgccggctctgatactagagcatcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgc BBa_M36762_sequence 1 atgaaatgggtgacctttatatctcttctctttttattcagctctgcttattcaaaggtatttgggaggtgtgagcttgccgctgctatgaagcgacacggattagacaactatcgaggatactccctcggtaattgggtttgtgctgcaaagtttgaaagtaactttaatacccaagccacgaatagaaatacagacgggtcaactgattacgggatccttcagataaatagcaggtggtggtgcaatgacggccgcactccaggatcacgtaatctttgcaacattccatgctcggcgttactttcgagtgacatcacagcgtcagtaaattgcgctaagaagatcgtgagtgatggcaatggcatgaacgcttgggtggcgtggcggaacaggtgtaaaggaacggacgttcaggcttggattagggggtgccggctctga BBa_K1462070_sequence 1 catcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z