BBa_M36627 1 BBa_M36627 GFP (modified from BBa_K648013) 2015-10-22T11:00:00Z 2015-10-23T06:30:43Z Source: BBa_K648013 This GFP part is completely based on BBa_K648013, with only 4 silent mutations (single-nucleotide substitutions) introduced for 2 purposes: 1. Removing BbsI, BsmBI, and BsaI restriction sites 2. Limit number of repeat sequences false false _848_ 29103 29103 9 false Design considerations: 1. Removing BbsI, BsmBI, and BsaI restriction sites 2. Limit number of repeat sequences false Benjamin Yeh, Jodie Sheffels, Ali Hoffer BBa_M36624 1 BBa_M36624 Antifungal protein from Aspergillus giganteus (active) 2015-10-21T11:00:00Z 2015-10-23T11:54:35Z http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1990.tb19300.x/full This links to the amino acid sequence, which we then entered into IDT Codon Optimizer to find the optimal corresponding DNA sequence in E. coli. This part codes for AFP, an antifungal protein produced naturally by Aspergillus giganteus, a mold species in the fungi kingdom. It appears to be involved in the inhibition of chitin synthesis, chitin being a key protein in the cell walls of fungi. It may also have other anti-fungal functions, but more research is necessary to determine its exact mode of action. false false _848_ 29100 29100 9 false The pre-protein is 94 amino acids long, but is cleaved before secretion into a 51 amino acid peptide that is now active. This part is for the 51 amino acid peptide plus a start codon. We have another part for the immature 94 amino acid peptide. false Jodie Sheffels, Benjamin Yeh, Ali Hoffer BBa_M36625 1 BBa_M36625 AFP Protein with GFP Tag 2015-10-22T11:00:00Z 2015-10-23T06:31:39Z AFP protein derived from Aspergillus giganteus. See Part:BBa_M36624 for more details. GFP protein derived from another Repository part, Part:BBa_K648013. See Part:BBa_M36029 for more details. AFP protein (51 AA peptide) tagged at the C-terminus with a standard GFP protein. Note: No start codon included. false false _848_ 29103 29103 9 false The construct was made to be within a 1500bp limit with less than 5 repeats of >= 10bp, and no BsaI, BbsI, or BsmBI restriction sites. false Benjamin Yeh, Jodie Sheffels, Ali Hoffer component2478322 1 BBa_M36627 component2478321 1 BBa_M36624 annotation2478322 1 BBa_M36627 range2478322 1 162 896 annotation2478321 1 BBa_M36624 range2478321 1 1 153 BBa_M36627_sequence 1 cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgagggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaggacggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagcgcgaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagattataaagatgatgacgacaag BBa_M36625_sequence 1 gccacctacaatggtaaatgctacaaaaaggataacatctgtaaatacaaagcgcagtccggcaaaaccgcaatttgcaaatgctatgtgaaaaaatgtccgcgtgatggagctaagtgcgagttcgacagctataaaggtaaatgttattgctactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgagggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaggacggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagcgcgaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagattataaagatgatgacgacaag BBa_M36624_sequence 1 gccacctacaatggtaaatgctacaaaaaggataacatctgtaaatacaaagcgcagtccggcaaaaccgcaatttgcaaatgctatgtgaaaaaatgtccgcgtgatggagctaagtgcgagttcgacagctataaaggtaaatgttattgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z