BBa_M36627 1 BBa_M36627 GFP (modified from BBa_K648013) 2015-10-22T11:00:00Z 2015-10-23T06:30:43Z Source: BBa_K648013 This GFP part is completely based on BBa_K648013, with only 4 silent mutations (single-nucleotide substitutions) introduced for 2 purposes: 1. Removing BbsI, BsmBI, and BsaI restriction sites 2. Limit number of repeat sequences false false _848_ 29103 29103 9 false Design considerations: 1. Removing BbsI, BsmBI, and BsaI restriction sites 2. Limit number of repeat sequences false Benjamin Yeh, Jodie Sheffels, Ali Hoffer BBa_M36626 1 BBa_M36626 AFP Pre-Protein with GFP Tag 2015-10-22T11:00:00Z 2015-10-23T06:33:46Z AFP protein derived from Aspergillus giganteus. See Part:BBa_M36623 for more details. GFP protein derived from another Repository part, Part:BBa_K648013. See Part:BBa_M36029 for more details. AFP protein (94 AA preproprotein version) tagged at the C-terminus with a standard GFP protein false false _848_ 29103 29103 9 No part sequence false The construct was made to be within a 1500bp limit with less than 5 repeats of >= 10bp and no BsaI, BbsI, or BsmBI restriction sites. false Benjamin Yeh, Jodie Sheffels, Ali Hoffer component2478325 1 BBa_M36623 component2478326 1 BBa_M36627 annotation2478325 1 BBa_M36623 range2478325 1 1 282 annotation2478326 1 BBa_M36627 range2478326 1 291 1025 BBa_M36623 1 BBa_M36623 Antifungal protein from Aspergillus giganteus 2015-10-21T11:00:00Z 2015-10-22T05:42:16Z http://www.ncbi.nlm.nih.gov/pubmed/8082203 This part codes for an antifungal protein produced naturally by Aspergillus giganteus, a mold species in the fungi kingdom. It appears to be involved in the inhibition of chitin synthesis (chitin being a protein that fungi rely on to build their cell walls). We plan to produce this protein in E. coli for potential use in combating plant-pathogenic fungi. false false _848_ 29103 29100 9 false This pre-protein is 94 amino acids upon translation, but is later cleaved into a 51 amino acid-long peptide. This 51 amino acid version is the active version. false Jodie Sheffels, Benjamin Yeh, Ali Hoffer BBa_M36626_sequence 1 atgaaattcgtgagcttagcgtccctggggttcgccctcgtggccgccttgggcgcggtggctaccccggttgaagcagattcgctgaccgcgggtgggctggacgcgcgcgatgaatccgcggtactggccacttataatggtaaatgttacaaaaaagacaatatctgcaagtacaaagcacagagtggcaaaaccgcgatctgtaaatgctatgtgaaaaaatgcccgcgcgacggcgccaaatgtgagtttgacagctataaaggtaaatgctattgctactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgagggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaggacggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagcgcgaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagattataaagatgatgacgacaag BBa_M36623_sequence 1 atgaaattcgtgagcttagcgtccctggggttcgccctcgtggccgccttgggcgcggtggctaccccggttgaagcagattcgctgaccgcgggtgggctggacgcgcgcgatgaatccgcggtactggccacttataatggtaaatgttacaaaaaagacaatatctgcaagtacaaagcacagagtggcaaaaccgcgatctgtaaatgctatgtgaaaaaatgcccgcgcgacggcgccaaatgtgagtttgacagctataaaggtaaatgctattgc BBa_M36627_sequence 1 cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgagggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaggacggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagcgcgaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagattataaagatgatgacgacaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z