BBa_M36658
1
BBa_M36658
mRNA Sequence for Acetoacetyl-CoA Reductase
2015-12-04T12:00:00Z
2015-12-04T06:52:29Z
Part comes from the genomic sequence of Phaeobacter inhibens (strain ATCC 700781/DSM 17395/CIP 105210/NBRC 16654/BS107).
Referenced http://www.uniprot.org/uniprot/G0CFI5 and http://www.google.com/patents/WO1995020621A1?cl=en, which both contributed to the discovery of the gene sequence for the necessary protein.
Part codes for the mRNA sequence to produce acetoacetyl-CoA reductase, which synthesizes poly-3-hydroxybutyrate in from acetoacetyl-CoA. This composite part is the DNA sequence collected from an amino acid sequence obtained from Phaeobacter inhibens (strain ATCC 700781/DSM 17395/CIP 105210/NBRC 16654/BS107), specifically from the gene I7EIV2. Part is intended to be inserted into pD454-GST, with a high copy number and ampicillin resistance. Complete system should be transformed into E. Coli.
false
false
_848_
29108
29108
9
false
Part was designed to meet the following conditions: be less than 1500 bp, avoid BbsI, BsaI, and BsmBI, and have less than five repeats 10 bp. There was an active attempt to avoid the restriction sites, stated above, that DNA 2.0 uses in the synthesis of DNA and to minimize repeats in the sequence to ensure success during synthesis. A limit of 1500 bp was imposed by the course.
false
Taylor Marie Chavez, Maria Iglesias, Alison Jahansouz
BBa_M36660
1
BBa_M36660
mRNA Sequence for Poly-beta-Hydroxybutyrate Polymerase
2015-10-22T11:00:00Z
2015-12-04T06:52:56Z
Part comes from the genomic sequence of Xanthomonas campestris pv. raphani 756C, specifically G0CFI5.
Referenced http://www.uniprot.org/uniprot/G0CFI5 and http://www.google.com/patents/WO1995020621A1?cl=en, which both contributed to the discovery of the gene sequence for the necessary protein.
Part codes for the mRNA sequence to produce poly-beta-hydroxybutyrate polymerase, which synthesizes poly-3-hydroxybutyrate in the presence of glucose. Poly-3-hydroxybutyrate, or P3HB, is a biodegradable polymer which will break down after it has fulfilled its intended use. Part is intended to be inserted into pD431-SR, with a high copy number and kanamycin resistance. Complete system should be transformed into E. Coli.
Amino Acid sequence is as follows: MKGPLGFSAEDLMQETLSMQRKLREGLKLLPGVEDVDYGVTERQEVWRDGKVVLYRFVGDAAPVARTPLLIVYALVNRPYMVDLQADRSLVKGLLGHGQDVYVLDWGYPDRSERYLTLEDYLLRYIDGAVDHLRAASGLEAVDVLGICQGGTFALCYAALERAKIRNLITMVTPVDFHTPDNMLSNWARMVDVDLFVDTMGNVPADLMNASYLMLKPFRLNLQKYVGLLDILDDKQALEDFLRMEKWIFDSPDLAGEAFREFVTQFYQRNGLVTGDVRIGGQAVDLHAVDMPVLNIYAEHDHLVPPDASRALRGLVGSEDYTELSFRGGHIGIYVSGRAQREVPVAIHRWLQARGGNG*
false
false
_848_
29108
29108
9
false
Part was designed to meet the following conditions: be less than 1500 bp, avoid BbsI, BsaI, and BsmBI, and have less than five repeats 10 bp. There was an active attempt to avoid the restriction sites, stated above, that DNA 2.0 uses in the synthesis of DNA and to minimize repeats in the sequence to ensure success during synthesis. A limit of 1500 bp was imposed by the course.
false
Taylor Marie Chavez, Maria Iglesias, Alison Jahansouz
BBa_M36659
1
BBa_M36659
Poly-3-hydroxybutyrate Growth Actuator
2015-10-22T11:00:00Z
2015-12-04T06:55:33Z
Xanthomonas campestris pv. raphani 756C is the organism from which the DNA sequence for poly-beta-hydroxybutyrate polymerase.
References include http://www.uniprot.org/uniprot/G0CFI5 and http://www.google.com/patents/WO1995020621A1?cl=en.
Part codes for the mRNA sequence to produce poly-beta-hydroxybutyrate polymerase, which synthesizes poly-3-hydroxybutyrate in the presence of glucose. This composite part is simply the DNA sequence collected from Xanthomonas campestris pv. raphani 756C, specifically from the gene G0CFI5.
false
false
_848_
29108
29108
9
false
Design considerations include limiting the DNA sequence to 1500 bp, avoiding BbsI, BsaI, and BsmBI, and ensuring less than five repeats of 10 bp. Restriction sites were avoided to not conflict with DNA 2.0 synthesis process, as was the minimization of repeats in the sequence.
false
Taylor Marie Chavez, Maria Iglesias, Alison Jahansouz
component2478433
1
BBa_M36658
component2478432
1
BBa_M36660
annotation2478433
1
BBa_M36658
range2478433
1
1084
1806
annotation2478432
1
BBa_M36660
range2478432
1
1
1077
BBa_M36660_sequence
1
atgaagggtcctcttggtttttctgccgaagatttgatgcaagagacgttgtccatgcagcgtaaactgcgtgaaggcctgaaactcctgcctggtgtggaggatgttgactatggtgttactgaacgccaggaagtttggcgcgacggcaaagtcgtcttgtatcgtttcgtcggagacgcggcaccagttgcacgtacaccgttgctgattgtgtatgctttagttaatcgtccttacatggtagatctgcaggccgaccgttccctcgttaaaggtctgttgggccatggccaggatgtgtatgtgctggattggggttatccggatcgttccgaacgttatctgacgctggaagattatttacttcgctacatcgatggcgccgttgaccatcttcgcgctgcaagtggtctggaggcggttgatgttctgggtatctgccaaggtgggacgtttgctctctgctacgcagcattagaacgcgctaaaatccgcaatctgatcaccatggtgaccccagtcgacttccacaccccggacaatatgttatcgaactgggcccgcatggtggacgttgatctgtttgtggataccatggggaatgtaccggcggatttaatgaacgcgagctatctcatgctgaaaccgttccgcctgaacctccaaaagtatgtgggattactggatattctcgatgacaagcaggccttagaagacttcctgcgtatggaaaaatggatctttgatagccccgacctcgcgggcgaagcattccgtgagttcgtgacccagttttatcagcgtaatggcctggtgaccggcgatgtccgtattggcggtcaagctgtggatctgcacgcagtggatatgccggtcctgaacatctatgcagaacatgatcatctggttccgccggacgcttctcgcgcgctgcgtggacttgtcgggtcggaagattataccgaactgtcatttcgtggagggcacattggtatttatgtctcggggcgcgcccagcgcgaagtgcctgtagctattcatcgttggttacaggcccgcggtggtaacggttga
BBa_M36659_sequence
1
atgaagggtcctcttggtttttctgccgaagatttgatgcaagagacgttgtccatgcagcgtaaactgcgtgaaggcctgaaactcctgcctggtgtggaggatgttgactatggtgttactgaacgccaggaagtttggcgcgacggcaaagtcgtcttgtatcgtttcgtcggagacgcggcaccagttgcacgtacaccgttgctgattgtgtatgctttagttaatcgtccttacatggtagatctgcaggccgaccgttccctcgttaaaggtctgttgggccatggccaggatgtgtatgtgctggattggggttatccggatcgttccgaacgttatctgacgctggaagattatttacttcgctacatcgatggcgccgttgaccatcttcgcgctgcaagtggtctggaggcggttgatgttctgggtatctgccaaggtgggacgtttgctctctgctacgcagcattagaacgcgctaaaatccgcaatctgatcaccatggtgaccccagtcgacttccacaccccggacaatatgttatcgaactgggcccgcatggtggacgttgatctgtttgtggataccatggggaatgtaccggcggatttaatgaacgcgagctatctcatgctgaaaccgttccgcctgaacctccaaaagtatgtgggattactggatattctcgatgacaagcaggccttagaagacttcctgcgtatggaaaaatggatctttgatagccccgacctcgcgggcgaagcattccgtgagttcgtgacccagttttatcagcgtaatggcctggtgaccggcgatgtccgtattggcggtcaagctgtggatctgcacgcagtggatatgccggtcctgaacatctatgcagaacatgatcatctggttccgccggacgcttctcgcgcgctgcgtggacttgtcgggtcggaagattataccgaactgtcatttcgtggagggcacattggtatttatgtctcggggcgcgcccagcgcgaagtgcctgtagctattcatcgttggttacaggcccgcggtggtaacggttgatactagatggcccgtaacgcgctggtcaccggcggtagtcgcgggatcggagcagccatctcacaggcgctgaaagcggagggctacaccgtggccgctacctacgctggcaatgacgaagcggcggcgaaattcacgaatgagactggcattaaaacatataaatgggatgttgcgagctatgaagattcggccgccggtatcgccaaggttgaagcggacattgggccgattgatattgttgtggcaaatgctgggattacccgtgatgctcccttccataaaatgacgctcgagcagtggcagcaggtcatagataccaacctcaccggtgtgtttaacaccgtgcatccgatctggcctggtatgcgcgaacgcaagttcggccgtgtgatcgtcatttcttccatcaacgggcaaaaagggcagtttgcgcaggtaaattatgcggctactaaagccggtgatcttggtatcgtgaaatccctggcgcaagaaggagctcgcgcgggcatcaccgccaatgccatttgccccggttatatcgcgacagaaatggtcatggccgttcctgaaaaggttcgtgagagcattatcggccagattcctgccggacgtttaggcgaacccgaagaaattgctcgttgcgtggcattcttggcgtccgaggactcgggctttattaacgggtctacaatcagcgcgaatggcgcccagtttttcgtataa
BBa_M36658_sequence
1
atggcccgtaacgcgctggtcaccggcggtagtcgcgggatcggagcagccatctcacaggcgctgaaagcggagggctacaccgtggccgctacctacgctggcaatgacgaagcggcggcgaaattcacgaatgagactggcattaaaacatataaatgggatgttgcgagctatgaagattcggccgccggtatcgccaaggttgaagcggacattgggccgattgatattgttgtggcaaatgctgggattacccgtgatgctcccttccataaaatgacgctcgagcagtggcagcaggtcatagataccaacctcaccggtgtgtttaacaccgtgcatccgatctggcctggtatgcgcgaacgcaagttcggccgtgtgatcgtcatttcttccatcaacgggcaaaaagggcagtttgcgcaggtaaattatgcggctactaaagccggtgatcttggtatcgtgaaatccctggcgcaagaaggagctcgcgcgggcatcaccgccaatgccatttgccccggttatatcgcgacagaaatggtcatggccgttcctgaaaaggttcgtgagagcattatcggccagattcctgccggacgtttaggcgaacccgaagaaattgctcgttgcgtggcattcttggcgtccgaggactcgggctttattaacgggtctacaatcagcgcgaatggcgcccagtttttcgtataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z