BBa_M36660 1 BBa_M36660 mRNA Sequence for Poly-beta-Hydroxybutyrate Polymerase 2015-10-22T11:00:00Z 2015-12-04T06:52:56Z Part comes from the genomic sequence of Xanthomonas campestris pv. raphani 756C, specifically G0CFI5. Referenced http://www.uniprot.org/uniprot/G0CFI5 and http://www.google.com/patents/WO1995020621A1?cl=en, which both contributed to the discovery of the gene sequence for the necessary protein. Part codes for the mRNA sequence to produce poly-beta-hydroxybutyrate polymerase, which synthesizes poly-3-hydroxybutyrate in the presence of glucose. Poly-3-hydroxybutyrate, or P3HB, is a biodegradable polymer which will break down after it has fulfilled its intended use. Part is intended to be inserted into pD431-SR, with a high copy number and kanamycin resistance. Complete system should be transformed into E. Coli. Amino Acid sequence is as follows: MKGPLGFSAEDLMQETLSMQRKLREGLKLLPGVEDVDYGVTERQEVWRDGKVVLYRFVGDAAPVARTPLLIVYALVNRPYMVDLQADRSLVKGLLGHGQDVYVLDWGYPDRSERYLTLEDYLLRYIDGAVDHLRAASGLEAVDVLGICQGGTFALCYAALERAKIRNLITMVTPVDFHTPDNMLSNWARMVDVDLFVDTMGNVPADLMNASYLMLKPFRLNLQKYVGLLDILDDKQALEDFLRMEKWIFDSPDLAGEAFREFVTQFYQRNGLVTGDVRIGGQAVDLHAVDMPVLNIYAEHDHLVPPDASRALRGLVGSEDYTELSFRGGHIGIYVSGRAQREVPVAIHRWLQARGGNG* false false _848_ 29108 29108 9 false Part was designed to meet the following conditions: be less than 1500 bp, avoid BbsI, BsaI, and BsmBI, and have less than five repeats 10 bp. There was an active attempt to avoid the restriction sites, stated above, that DNA 2.0 uses in the synthesis of DNA and to minimize repeats in the sequence to ensure success during synthesis. A limit of 1500 bp was imposed by the course. false Taylor Marie Chavez, Maria Iglesias, Alison Jahansouz BBa_M36660_sequence 1 atgaagggtcctcttggtttttctgccgaagatttgatgcaagagacgttgtccatgcagcgtaaactgcgtgaaggcctgaaactcctgcctggtgtggaggatgttgactatggtgttactgaacgccaggaagtttggcgcgacggcaaagtcgtcttgtatcgtttcgtcggagacgcggcaccagttgcacgtacaccgttgctgattgtgtatgctttagttaatcgtccttacatggtagatctgcaggccgaccgttccctcgttaaaggtctgttgggccatggccaggatgtgtatgtgctggattggggttatccggatcgttccgaacgttatctgacgctggaagattatttacttcgctacatcgatggcgccgttgaccatcttcgcgctgcaagtggtctggaggcggttgatgttctgggtatctgccaaggtgggacgtttgctctctgctacgcagcattagaacgcgctaaaatccgcaatctgatcaccatggtgaccccagtcgacttccacaccccggacaatatgttatcgaactgggcccgcatggtggacgttgatctgtttgtggataccatggggaatgtaccggcggatttaatgaacgcgagctatctcatgctgaaaccgttccgcctgaacctccaaaagtatgtgggattactggatattctcgatgacaagcaggccttagaagacttcctgcgtatggaaaaatggatctttgatagccccgacctcgcgggcgaagcattccgtgagttcgtgacccagttttatcagcgtaatggcctggtgaccggcgatgtccgtattggcggtcaagctgtggatctgcacgcagtggatatgccggtcctgaacatctatgcagaacatgatcatctggttccgccggacgcttctcgcgcgctgcgtggacttgtcgggtcggaagattataccgaactgtcatttcgtggagggcacattggtatttatgtctcggggcgcgcccagcgcgaagtgcctgtagctattcatcgttggttacaggcccgcggtggtaacggttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z