LOCUS       BBa_M36666              1227 bp    DNA     linear       23-Dec-2024
DEFINITION  HeLa beta-galactosidase actuator with secretion tag
ACCESSION   BBa_M36666
VERSION     BBa_M36666.1  
FEATURES             Location/Qualifiers
     misc_feature    1..12
                     /label=Kozak sequence
     misc_feature    13..15
                     /label=start codon
     misc_feature    16..57
                     /label=PPT secretion tag
     CDS             58..1101
                     /label=HeLa-optimized beta-galactosidase
     CDS             58..237
                     /label=lacZ alpha fragment
     CDS             238..1101
                     /label=lacZ omega fragment
     polyA_site      1102..1227
                     /label=poly-A tail
ORIGIN
        1 cccgccgcca ccatgtctgc cctgctgatt ctggccctcg tgggagccgc cgtggccatg
       61 acaatgatta ctgattcttt agcagtcgta ctgcaaagac gcgattggga aaacccaggc
      121 gtcacacagt tgaaccgttt ggccgcccat cctcctttcg cctcatggcg aaactcagag
      181 gaggccagga cagatcgccc atcacagcag ctgaggtcct taaacggcga atggaggatc
      241 ccccacctga cgacttccga gatggacttt tgtatcgagt tgggcaacaa acgctggcag
      301 ttcaatagac aatccggatt tctcagccaa atgtggattg gggataaaaa acagctgctg
      361 actcctctaa gagatcagtt cacccgcgct cccctggata atgacatcgg ggtctctgag
      421 gccactcgta tcgaccctaa cgcttgggtg gagcgctgga aagcggcggg ccactatcag
      481 gccgaggccg cactgctcca gtgcacagcc gacactctag ccgacgccgt gctcatcact
      541 acggcccatg catggcaaca ccaaggaaaa acccttttca tatctaggaa aacctacagg
      601 attgatggtt cggggcagat ggcaattacc gtggatgtgg aagtggcctc cgacacaccg
      661 caccctgctc ggattggcct caattgccaa ctcgcacagg tggccgagcg agtaaattgg
      721 ttaggcctcg ggccccagga gaactaccct gaccggctca cagcggcctg ttttgatagg
      781 tgggatctgc cgttatctga tatgtacaca ccatacgtgt ttccctccga gaatgggctc
      841 cgatgcggga cacgagagct taattatggc cctcaccagt ggcggggaga cttccaattt
      901 aacatcagca gatactcaca acagcagctg atggagacca gccacaggca cctgttgcac
      961 gctgaagaag gaacttggct caacatcgat ggctttcata tgggtattgg aggtgacgat
     1021 agctggtctc cctcagtgtc cgcggaattt cagctcagtg caggtaggta tcactaccag
     1081 ctggtgtggt gccaaaaata aaaaatcagc ctcgactgtg ccttctagtt gccagccatc
     1141 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     1201 ttcctaataa aatgaggaaa ttgcatc
//