LOCUS BBa_M36666 1227 bp DNA linear 29-Mar-2024 DEFINITION HeLa beta-galactosidase actuator with secretion tag ACCESSION BBa_M36666 VERSION BBa_M36666.1 FEATURES Location/Qualifiers misc_feature 1..12 /label=Kozak sequence misc_feature 13..15 /label=start codon misc_feature 16..57 /label=PPT secretion tag CDS 58..1101 /label=HeLa-optimized beta-galactosidase CDS 58..237 /label=lacZ alpha fragment CDS 238..1101 /label=lacZ omega fragment polyA_site 1102..1227 /label=poly-A tail ORIGIN 1 cccgccgcca ccatgtctgc cctgctgatt ctggccctcg tgggagccgc cgtggccatg 61 acaatgatta ctgattcttt agcagtcgta ctgcaaagac gcgattggga aaacccaggc 121 gtcacacagt tgaaccgttt ggccgcccat cctcctttcg cctcatggcg aaactcagag 181 gaggccagga cagatcgccc atcacagcag ctgaggtcct taaacggcga atggaggatc 241 ccccacctga cgacttccga gatggacttt tgtatcgagt tgggcaacaa acgctggcag 301 ttcaatagac aatccggatt tctcagccaa atgtggattg gggataaaaa acagctgctg 361 actcctctaa gagatcagtt cacccgcgct cccctggata atgacatcgg ggtctctgag 421 gccactcgta tcgaccctaa cgcttgggtg gagcgctgga aagcggcggg ccactatcag 481 gccgaggccg cactgctcca gtgcacagcc gacactctag ccgacgccgt gctcatcact 541 acggcccatg catggcaaca ccaaggaaaa acccttttca tatctaggaa aacctacagg 601 attgatggtt cggggcagat ggcaattacc gtggatgtgg aagtggcctc cgacacaccg 661 caccctgctc ggattggcct caattgccaa ctcgcacagg tggccgagcg agtaaattgg 721 ttaggcctcg ggccccagga gaactaccct gaccggctca cagcggcctg ttttgatagg 781 tgggatctgc cgttatctga tatgtacaca ccatacgtgt ttccctccga gaatgggctc 841 cgatgcggga cacgagagct taattatggc cctcaccagt ggcggggaga cttccaattt 901 aacatcagca gatactcaca acagcagctg atggagacca gccacaggca cctgttgcac 961 gctgaagaag gaacttggct caacatcgat ggctttcata tgggtattgg aggtgacgat 1021 agctggtctc cctcagtgtc cgcggaattt cagctcagtg caggtaggta tcactaccag 1081 ctggtgtggt gccaaaaata aaaaatcagc ctcgactgtg ccttctagtt gccagccatc 1141 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 1201 ttcctaataa aatgaggaaa ttgcatc //