BBa_M36666 1 BBa_M36666 HeLa beta-galactosidase actuator with secretion tag 2014-04-29T11:00:00Z 2015-05-08T01:14:05Z Kozak sequence obtained from Part:BBa_J63003. Secretion tag obtained from Part:BBa_K782062. LacZ alpha fragment obtained from Part:BBa_E0051. LacZ omega fragment obtained from Part:BBa_K891003. Poly-A tail obtained from Part:BBa_M36096. This actuator is designed to produce beta-galactosidase with an N-terminal secretion tag in HeLa cells. It links the alpha and omega fragments of the E. coli lacZ gene, though the codons have been optimized for expression in HeLa cells. It includes the kozak sequence immediately before the start codon and a secretion tag immediately after the start codon. false false _848_ 0 22139 9 Not in stock false The lacZ alpha fragment and lacZ omega fragment were both codon optimized for expression in HeLa cells. After combining all the parts, there were only 7 repeats of ≥10 base pairs, so in order to maintain codon optimization, no further changes were made. false Ian Hull, Poorwa Godbole annotation2372773 1 poly-A tail range2372773 1 1102 1227 annotation2372768 1 start codon range2372768 1 13 15 annotation2372772 1 lacZ omega fragment range2372772 1 238 1101 annotation2372770 1 HeLa-optimized beta-galactosidase range2372770 1 58 1101 annotation2372769 1 PPT secretion tag range2372769 1 16 57 annotation2372771 1 lacZ alpha fragment range2372771 1 58 237 annotation2372767 1 Kozak sequence range2372767 1 1 12 BBa_M36666_sequence 1 cccgccgccaccatgtctgccctgctgattctggccctcgtgggagccgccgtggccatgacaatgattactgattctttagcagtcgtactgcaaagacgcgattgggaaaacccaggcgtcacacagttgaaccgtttggccgcccatcctcctttcgcctcatggcgaaactcagaggaggccaggacagatcgcccatcacagcagctgaggtccttaaacggcgaatggaggatcccccacctgacgacttccgagatggacttttgtatcgagttgggcaacaaacgctggcagttcaatagacaatccggatttctcagccaaatgtggattggggataaaaaacagctgctgactcctctaagagatcagttcacccgcgctcccctggataatgacatcggggtctctgaggccactcgtatcgaccctaacgcttgggtggagcgctggaaagcggcgggccactatcaggccgaggccgcactgctccagtgcacagccgacactctagccgacgccgtgctcatcactacggcccatgcatggcaacaccaaggaaaaacccttttcatatctaggaaaacctacaggattgatggttcggggcagatggcaattaccgtggatgtggaagtggcctccgacacaccgcaccctgctcggattggcctcaattgccaactcgcacaggtggccgagcgagtaaattggttaggcctcgggccccaggagaactaccctgaccggctcacagcggcctgttttgataggtgggatctgccgttatctgatatgtacacaccatacgtgtttccctccgagaatgggctccgatgcgggacacgagagcttaattatggccctcaccagtggcggggagacttccaatttaacatcagcagatactcacaacagcagctgatggagaccagccacaggcacctgttgcacgctgaagaaggaacttggctcaacatcgatggctttcatatgggtattggaggtgacgatagctggtctccctcagtgtccgcggaatttcagctcagtgcaggtaggtatcactaccagctggtgtggtgccaaaaataaaaaatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z