LOCUS       BBa_M36689              2567 bp    DNA     linear       18-Apr-2024
DEFINITION  HSE 2x Promoter + Bxb1 Recombinase w/ NLS + SV40 Terminator + attB 
            site + CMV Reverse + attP site
ACCESSION   BBa_M36689
VERSION     BBa_M36689.1  
FEATURES             Location/Qualifiers
     promoter        1..98
                     /label=BBa_M36765
     CDS             105..1604
                     /label=Bxb1 - NLS No stop
     CDS             1605..1625
                     /label=NLS
     misc_feature    1626..1628
                     /label=STOP
     CDS             105..1628
                     /label=BBa_M36670
     misc_feature    1637..1874
                     /label=terminator
     misc_feature    1637..1874
                     /label=terminator
     terminator      1637..1874
                     /label=BBa_J52016
     misc_feature    1883..1922
                     /label=attB
     misc_feature    1883..1922
                     /label=BBa_M36676
     promoter        1931..2519
                     /label=CMV Promoter
     promoter        1931..2519
                     /label=BBa_M36769
     misc_feature    2528..2567
                     /label=attP
     misc_feature    2528..2567
                     /label=BBa_M36864
ORIGIN
        1 gtcaagaacg ttctagaacg tcaagaacgt tctagaacgt catataaaag cccaggggca
       61 agcggtccgg ataacggcta gcctgaggag ctgctgcgta ctagatgaga gccctggtag
      121 tcatccgcct gtcccgcgtc accgatgcta cgacttcacc ggagcgtcag ctggagtctt
      181 gccagcagct ctgcgcccag cgcggctggg acgtcgtcgg ggtagcggag gatctggacg
      241 tctccggggc ggtcgatccg ttcgaccgga agcgcagacc gaacctggcc cggtggctag
      301 cgttcgagga gcaaccgttc gacgtgatcg tggcgtaccg ggtagaccgg ttgacccgat
      361 cgatccggca tctgcaacag ctggtccact gggccgagga ccacaagaag ctggtcgtct
      421 ccgcgaccga agcgcacttc gatacgacga cgccgtttgc ggcggtcgtc atcgcgctta
      481 tgggaacggt ggcgcagatg gaattagaag cgatcaaaga gcggaaccgt tcggctgcgc
      541 atttcaatat ccgcgccggg aaataccgag gatccctgcc gccgtgggga tacctgccta
      601 cgcgcgtgga cggggagtgg cggctggtgc cggaccctgt gcagcgagag cgcatcctcg
      661 aggtgtatca ccgcgtcgtc gacaaccacg agccgctgca cctggtggcc cacgacctga
      721 accggcgtgg tgtcctgtcg ccgaaggact acttcgcgca gctgcaaggc cgcgagccgc
      781 agggccggga gtggtcggct accgcgctga agcgatcgat gatctccgag gcgatgctcg
      841 ggtacgcgac tctgaacggt aagaccgtcc gagacgacga cggagccccg ctggtgcggg
      901 ctgagccgat cctgacccgt gagcagctgg aggcgctgcg cgccgagctc gtgaagacct
      961 cccgggcgaa gcccgcggtg tctaccccgt cgctgctgct gcgggtgttg ttctgcgcgg
     1021 tgtgcgggga gcccgcgtac aagttcgccg ggggaggacg taagcacccg cgctaccgct
     1081 gccgctcgat ggggttcccg aagcactgcg ggaacggcac ggtggcgatg gccgagtggg
     1141 acgcgttctg cgaggagcag gtgctggatc tgctcgggga cgcggagcgt ctggagaaag
     1201 tctgggtagc cggctcggac tccgcggtcg aactcgcgga ggtgaacgcg gagctggtgg
     1261 acctgacgtc gctgatcggc tccccggcct accgggccgg ctctccgcag cgagaagcac
     1321 tggatgcccg tattgcggcg ctggccgcgc ggcaagagga gctggagggc ctagaggctc
     1381 gcccgtctgg ctgggagtgg cgcgagaccg ggcagcggtt cggggactgg tggcgggagc
     1441 aggacaccgc ggcaaagaac acctggcttc ggtcgatgaa cgttcggctg acgttcgacg
     1501 tccgcggcgg gctgactcgc acgatcgact tcggggatct gcaagagtac gagcagcatc
     1561 tcaggctcgg cagcgtggtc gaacggctac acaccgggat gtcgcctgca agaaaaagat
     1621 tgaattagta ctagagctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     1681 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     1741 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     1801 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     1861 gctggggata tgcatactag agtttcggat caagctatga aggacgcaaa gagggaacta
     1921 aatactagag gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta
     1981 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     2041 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     2101 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     2161 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     2221 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     2281 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     2341 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     2401 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     2461 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactat
     2521 actagagttc ctcgttttct ctcgttggaa gaagaagaaa cgagaaa
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