BBa_J52016 1 BBa_J52016 eukaryotic -- derived from SV40 early poly A signal sequence 2006-10-13T11:00:00Z 2015-08-31T03:54:04Z - - false false _80_ 0 800 80 It's complicated true It is cloned in BioBrick vector pSB1AK3. false Monika Ciglic annotation1902870 1 terminator range1902870 1 1 238 annotation1902869 1 terminator range1902869 1 1 238 BBa_M36670 1 BBa_M36670 Bxb1 Recombinase + NLS on N terminus 2014-10-23T11:00:00Z 2015-05-08T01:14:05Z http://parts.igem.org/Part:BBa_K907000 For Bxb1 sequence This is a Bxb1 integrase with a Nuclear Localization Site added to the N terminus, prior to the stop codon. This NLS should allow Bxb1 to be integrated into the nucleus of a eukaryotic chassis organism. Bxb1 is part of the serine integrase family, which are longer proteins and have relatively shorter identification sequences compared to tyrosine integrates. Bxb1 and φC31 are two prominent serine recombinases, and both have been shown to work within mammalian cell lines. When used as an invertase, Bxb1 identifies attB and attP sites, cuts at these sites, and inverts the DNA sequence in between these two markers. Bb1 integrase is capable of performing this process without additional cofactors, which has been confirmed by in vitro studies. false false _848_ 0 24144 9 Not in stock false Added a NLS to Bxb1 integrase to help promote accurate localization to the nucleus false Richard Fuisz annotation2429304 1 Bxb1 - NLS No stop range2429304 1 1 1500 annotation2429307 1 STOP range2429307 1 1522 1524 annotation2429306 1 NLS range2429306 1 1501 1521 BBa_M36765 1 BBa_M36765 Optimized Heat Shock Promoter (2x HSE) 2014-10-23T11:00:00Z 2015-05-08T01:14:06Z http://www.ncbi.nlm.nih.gov/pubmed/25168173 This part is an optimized Heat Shock Expression (HSE) promoter that has been developed as a replacement for HSPA1a, a common promoter used for detection of the transcription factor HSF1. These Heat Shock Proteins (HSPs) are crucial for preventing cell death/enhancing survival. In the study where this sequence was developed, the authors describe a repeating sequence of HSE elements, measuring the change in response with respect to number of elements incorporated. Although 5 HSE's were identified to optimize HSP expression, this sequence uses 2 HSE elements. These HSE's are more efficient due to selective detection of Heat Shock pathway activity as opposed to their HSPA1a counterparts which have other confounding stress factors included. false false _848_ 0 24156 9 Not in stock false Because we needed to synthesize this sequence, we only used 2x HSE's due to the repetitive nature of this promoter. If users would like a stronger heat shock recognizing promoter, refer to the source provided. false Shankara Anand BBa_M36864 1 BBa_M36864 attP site for Bxb1 Integrase 2014-10-23T11:00:00Z 2015-05-08T01:14:06Z http://www.sciencedirect.com/science/article/pii/S0022283603013561 attB and attP are identified by Bxb1 integrase to determine the bounds for cutting. For an invertase switch, place attP, then the promoter of interest upstream, then att. upstream of the gene. When used in an invertase switch, Bxb1 cuts the intervening code and substitutes attL and attR sites at attB and attP, respectively. Bxb1 will not cut attL and attP sites without additional cofactors. false false _848_ 0 24144 9 Not in stock false May not be the most condensed attP sequence false Richard Fuisz annotation2429312 1 attP range2429312 1 1 40 BBa_M36689 1 BBa_M36689 HSE 2x Promoter + Bxb1 Recombinase w/ NLS + SV40 Terminator + attB site + CMV Reverse + attP site 2014-10-23T11:00:00Z 2015-05-08T01:14:05Z HSE 2x: http://parts.igem.org/Part:BBa_M36765; http://www.ncbi.nlm.nih.gov/pubmed/25168173 Bxb1 Integrase: http://parts.igem.org/Part:BBa_M36670; http://parts.igem.org/Part:BBa_K907000 attB & attP: http://link.springer.com/article/10.1007/s00438-006-0129-5 CMV Reverse Complement: http://parts.igem.org/Part:BBa_M36769; http://parts.igem.org/Part:BBa_I712004 This part incorporates a HSE 2x optimized promoter for a highly elucidated heat shock transcription factor. Once this site has been activated, it signals a Bxb1 Recombinase, a serine based recombinase. When used as an invertase, Bxb1 identifies attB and attP sites, cuts at these sites, and inverts the DNA sequence in between these two markers. Bb1 integrase is capable of performing this process without additional cofactors, which has been confirmed by in vitro studies. The attB and attP sites are both incorporated into this composite with a CMV reverse complement promoter within. This reverse promoter will, as a result, be switched "on" once activated by the HSE 2x transcription factor site and may be used to activated some sort of sensor. false false _848_ 0 24156 9 Not in stock false This composite was designed for use in mammalian cells. As a result, we've incorporated an NLS within our Bxb1 Recombinase to ensure compatibility. Additionally, our serine based recombinase used here has been proven in studies to work properly with our attB and attP recognition sites. false Shankara Anand component2429501 1 BBa_M36670 component2429508 1 BBa_M36769 component2429504 1 BBa_J52016 component2429506 1 BBa_M36676 component2429497 1 BBa_M36765 component2429510 1 BBa_M36864 annotation2429504 1 BBa_J52016 range2429504 1 1637 1874 annotation2429497 1 BBa_M36765 range2429497 1 1 98 annotation2429501 1 BBa_M36670 range2429501 1 105 1628 annotation2429506 1 BBa_M36676 range2429506 1 1883 1922 annotation2429510 1 BBa_M36864 range2429510 1 2528 2567 annotation2429508 1 BBa_M36769 range2429508 1 1931 2519 BBa_M36676 1 BBa_M36676 attB site for Bxb1 integrase 2014-10-23T11:00:00Z 2015-05-08T01:14:05Z http://www.sciencedirect.com/science/article/pii/S0022283603013561 attB and attP are identified by Bxb1 integrase to determine the bounds for cutting. For an invertase switch, place attP, then the promoter of interest upstream, then att. upstream of the gene. When used in an invertase switch, Bxb1 cuts the intervening code and substitutes attL and attR sites at attB and attP, respectively. Bxb1 will not cut attL and attP sites without additional cofactors. false false _848_ 0 24144 9 Not in stock false Similar sequences already exist on parts registry- this may not be the most condensed sequence available. false Richard Fuisz annotation2429311 1 attB range2429311 1 1 40 BBa_M36769 1 BBa_M36769 CMV Promoter Reverse Complement 2014-10-23T11:00:00Z 2015-05-08T01:14:06Z http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010611 This is the REVERSE COMPLEMENT of the eukaryotic promoter CMV, for use in invertase switches. CMV is a relatively strong constitutive promoter. If looking for a CMV Promoter that is faced forward, and for more information on the CMV promoter consult BBa_I712004 http://parts.igem.org/Part:BBa_I712004 false false _848_ 0 24144 9 Not in stock false This is NOT the CMV promoter- it is the reverse complement of a CMV promoter sequence. false Richard Fuisz annotation2429310 1 CMV Promoter range2429310 1 1 589 BBa_M36689_sequence 1 gtcaagaacgttctagaacgtcaagaacgttctagaacgtcatataaaagcccaggggcaagcggtccggataacggctagcctgaggagctgctgcgtactagatgagagccctggtagtcatccgcctgtcccgcgtcaccgatgctacgacttcaccggagcgtcagctggagtcttgccagcagctctgcgcccagcgcggctgggacgtcgtcggggtagcggaggatctggacgtctccggggcggtcgatccgttcgaccggaagcgcagaccgaacctggcccggtggctagcgttcgaggagcaaccgttcgacgtgatcgtggcgtaccgggtagaccggttgacccgatcgatccggcatctgcaacagctggtccactgggccgaggaccacaagaagctggtcgtctccgcgaccgaagcgcacttcgatacgacgacgccgtttgcggcggtcgtcatcgcgcttatgggaacggtggcgcagatggaattagaagcgatcaaagagcggaaccgttcggctgcgcatttcaatatccgcgccgggaaataccgaggatccctgccgccgtggggatacctgcctacgcgcgtggacggggagtggcggctggtgccggaccctgtgcagcgagagcgcatcctcgaggtgtatcaccgcgtcgtcgacaaccacgagccgctgcacctggtggcccacgacctgaaccggcgtggtgtcctgtcgccgaaggactacttcgcgcagctgcaaggccgcgagccgcagggccgggagtggtcggctaccgcgctgaagcgatcgatgatctccgaggcgatgctcgggtacgcgactctgaacggtaagaccgtccgagacgacgacggagccccgctggtgcgggctgagccgatcctgacccgtgagcagctggaggcgctgcgcgccgagctcgtgaagacctcccgggcgaagcccgcggtgtctaccccgtcgctgctgctgcgggtgttgttctgcgcggtgtgcggggagcccgcgtacaagttcgccgggggaggacgtaagcacccgcgctaccgctgccgctcgatggggttcccgaagcactgcgggaacggcacggtggcgatggccgagtgggacgcgttctgcgaggagcaggtgctggatctgctcggggacgcggagcgtctggagaaagtctgggtagccggctcggactccgcggtcgaactcgcggaggtgaacgcggagctggtggacctgacgtcgctgatcggctccccggcctaccgggccggctctccgcagcgagaagcactggatgcccgtattgcggcgctggccgcgcggcaagaggagctggagggcctagaggctcgcccgtctggctgggagtggcgcgagaccgggcagcggttcggggactggtggcgggagcaggacaccgcggcaaagaacacctggcttcggtcgatgaacgttcggctgacgttcgacgtccgcggcgggctgactcgcacgatcgacttcggggatctgcaagagtacgagcagcatctcaggctcggcagcgtggtcgaacggctacacaccgggatgtcgcctgcaagaaaaagattgaattagtactagagctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatatgcatactagagtttcggatcaagctatgaaggacgcaaagagggaactaaatactagaggatctgacggttcactaaaccagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgcggaactccatatatgggctatgaactaatgaccccgtaattgattactattaataactatactagagttcctcgttttctctcgttggaagaagaagaaacgagaaa BBa_M36864_sequence 1 ttcctcgttttctctcgttggaagaagaagaaacgagaaa BBa_M36769_sequence 1 gatctgacggttcactaaaccagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgcggaactccatatatgggctatgaactaatgaccccgtaattgattactattaataacta BBa_M36670_sequence 1 atgagagccctggtagtcatccgcctgtcccgcgtcaccgatgctacgacttcaccggagcgtcagctggagtcttgccagcagctctgcgcccagcgcggctgggacgtcgtcggggtagcggaggatctggacgtctccggggcggtcgatccgttcgaccggaagcgcagaccgaacctggcccggtggctagcgttcgaggagcaaccgttcgacgtgatcgtggcgtaccgggtagaccggttgacccgatcgatccggcatctgcaacagctggtccactgggccgaggaccacaagaagctggtcgtctccgcgaccgaagcgcacttcgatacgacgacgccgtttgcggcggtcgtcatcgcgcttatgggaacggtggcgcagatggaattagaagcgatcaaagagcggaaccgttcggctgcgcatttcaatatccgcgccgggaaataccgaggatccctgccgccgtggggatacctgcctacgcgcgtggacggggagtggcggctggtgccggaccctgtgcagcgagagcgcatcctcgaggtgtatcaccgcgtcgtcgacaaccacgagccgctgcacctggtggcccacgacctgaaccggcgtggtgtcctgtcgccgaaggactacttcgcgcagctgcaaggccgcgagccgcagggccgggagtggtcggctaccgcgctgaagcgatcgatgatctccgaggcgatgctcgggtacgcgactctgaacggtaagaccgtccgagacgacgacggagccccgctggtgcgggctgagccgatcctgacccgtgagcagctggaggcgctgcgcgccgagctcgtgaagacctcccgggcgaagcccgcggtgtctaccccgtcgctgctgctgcgggtgttgttctgcgcggtgtgcggggagcccgcgtacaagttcgccgggggaggacgtaagcacccgcgctaccgctgccgctcgatggggttcccgaagcactgcgggaacggcacggtggcgatggccgagtgggacgcgttctgcgaggagcaggtgctggatctgctcggggacgcggagcgtctggagaaagtctgggtagccggctcggactccgcggtcgaactcgcggaggtgaacgcggagctggtggacctgacgtcgctgatcggctccccggcctaccgggccggctctccgcagcgagaagcactggatgcccgtattgcggcgctggccgcgcggcaagaggagctggagggcctagaggctcgcccgtctggctgggagtggcgcgagaccgggcagcggttcggggactggtggcgggagcaggacaccgcggcaaagaacacctggcttcggtcgatgaacgttcggctgacgttcgacgtccgcggcgggctgactcgcacgatcgacttcggggatctgcaagagtacgagcagcatctcaggctcggcagcgtggtcgaacggctacacaccgggatgtcgcctgcaagaaaaagattgaattag BBa_M36765_sequence 1 gtcaagaacgttctagaacgtcaagaacgttctagaacgtcatataaaagcccaggggcaagcggtccggataacggctagcctgaggagctgctgcg BBa_J52016_sequence 1 ctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatatgca BBa_M36676_sequence 1 tttcggatcaagctatgaaggacgcaaagagggaactaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z