BBa_J52016
1
BBa_J52016
eukaryotic -- derived from SV40 early poly A signal sequence
2006-10-13T11:00:00Z
2015-08-31T03:54:04Z
-
-
false
false
_80_
0
800
80
It's complicated
true
It is cloned in BioBrick vector pSB1AK3.
false
Monika Ciglic
annotation1902870
1
terminator
range1902870
1
1
238
annotation1902869
1
terminator
range1902869
1
1
238
BBa_M36670
1
BBa_M36670
Bxb1 Recombinase + NLS on N terminus
2014-10-23T11:00:00Z
2015-05-08T01:14:05Z
http://parts.igem.org/Part:BBa_K907000 For Bxb1 sequence
This is a Bxb1 integrase with a Nuclear Localization Site added to the N terminus, prior to the stop codon. This NLS should allow Bxb1 to be integrated into the nucleus of a eukaryotic chassis organism.
Bxb1 is part of the serine integrase family, which are longer proteins and have relatively shorter identification sequences compared to tyrosine integrates. Bxb1 and φC31 are two prominent serine recombinases, and both have been shown to work within mammalian cell lines.
When used as an invertase, Bxb1 identifies attB and attP sites, cuts at these sites, and inverts the DNA sequence in between these two markers. Bb1 integrase is capable of performing this process without additional cofactors, which has been confirmed by in vitro studies.
false
false
_848_
0
24144
9
Not in stock
false
Added a NLS to Bxb1 integrase to help promote accurate localization to the nucleus
false
Richard Fuisz
annotation2429304
1
Bxb1 - NLS No stop
range2429304
1
1
1500
annotation2429307
1
STOP
range2429307
1
1522
1524
annotation2429306
1
NLS
range2429306
1
1501
1521
BBa_M36765
1
BBa_M36765
Optimized Heat Shock Promoter (2x HSE)
2014-10-23T11:00:00Z
2015-05-08T01:14:06Z
http://www.ncbi.nlm.nih.gov/pubmed/25168173
This part is an optimized Heat Shock Expression (HSE) promoter that has been developed as a replacement for HSPA1a, a common promoter used for detection of the transcription factor HSF1. These Heat Shock Proteins (HSPs) are crucial for preventing cell death/enhancing survival. In the study where this sequence was developed, the authors describe a repeating sequence of HSE elements, measuring the change in response with respect to number of elements incorporated. Although 5 HSE's were identified to optimize HSP expression, this sequence uses 2 HSE elements.
These HSE's are more efficient due to selective detection of Heat Shock pathway activity as opposed to their HSPA1a counterparts which have other confounding stress factors included.
false
false
_848_
0
24156
9
Not in stock
false
Because we needed to synthesize this sequence, we only used 2x HSE's due to the repetitive nature of this promoter. If users would like a stronger heat shock recognizing promoter, refer to the source provided.
false
Shankara Anand
BBa_M36864
1
BBa_M36864
attP site for Bxb1 Integrase
2014-10-23T11:00:00Z
2015-05-08T01:14:06Z
http://www.sciencedirect.com/science/article/pii/S0022283603013561
attB and attP are identified by Bxb1 integrase to determine the bounds for cutting. For an invertase switch, place attP, then the promoter of interest upstream, then att. upstream of the gene. When used in an invertase switch, Bxb1 cuts the intervening code and substitutes attL and attR sites at attB and attP, respectively. Bxb1 will not cut attL and attP sites without additional cofactors.
false
false
_848_
0
24144
9
Not in stock
false
May not be the most condensed attP sequence
false
Richard Fuisz
annotation2429312
1
attP
range2429312
1
1
40
BBa_M36689
1
BBa_M36689
HSE 2x Promoter + Bxb1 Recombinase w/ NLS + SV40 Terminator + attB site + CMV Reverse + attP site
2014-10-23T11:00:00Z
2015-05-08T01:14:05Z
HSE 2x: http://parts.igem.org/Part:BBa_M36765; http://www.ncbi.nlm.nih.gov/pubmed/25168173
Bxb1 Integrase: http://parts.igem.org/Part:BBa_M36670; http://parts.igem.org/Part:BBa_K907000
attB & attP: http://link.springer.com/article/10.1007/s00438-006-0129-5
CMV Reverse Complement: http://parts.igem.org/Part:BBa_M36769; http://parts.igem.org/Part:BBa_I712004
This part incorporates a HSE 2x optimized promoter for a highly elucidated heat shock transcription factor. Once this site has been activated, it signals a Bxb1 Recombinase, a serine based recombinase. When used as an invertase, Bxb1 identifies attB and attP sites, cuts at these sites, and inverts the DNA sequence in between these two markers. Bb1 integrase is capable of performing this process without additional cofactors, which has been confirmed by in vitro studies. The attB and attP sites are both incorporated into this composite with a CMV reverse complement promoter within. This reverse promoter will, as a result, be switched "on" once activated by the HSE 2x transcription factor site and may be used to activated some sort of sensor.
false
false
_848_
0
24156
9
Not in stock
false
This composite was designed for use in mammalian cells. As a result, we've incorporated an NLS within our Bxb1 Recombinase to ensure compatibility. Additionally, our serine based recombinase used here has been proven in studies to work properly with our attB and attP recognition sites.
false
Shankara Anand
component2429501
1
BBa_M36670
component2429508
1
BBa_M36769
component2429504
1
BBa_J52016
component2429506
1
BBa_M36676
component2429497
1
BBa_M36765
component2429510
1
BBa_M36864
annotation2429504
1
BBa_J52016
range2429504
1
1637
1874
annotation2429497
1
BBa_M36765
range2429497
1
1
98
annotation2429501
1
BBa_M36670
range2429501
1
105
1628
annotation2429506
1
BBa_M36676
range2429506
1
1883
1922
annotation2429510
1
BBa_M36864
range2429510
1
2528
2567
annotation2429508
1
BBa_M36769
range2429508
1
1931
2519
BBa_M36676
1
BBa_M36676
attB site for Bxb1 integrase
2014-10-23T11:00:00Z
2015-05-08T01:14:05Z
http://www.sciencedirect.com/science/article/pii/S0022283603013561
attB and attP are identified by Bxb1 integrase to determine the bounds for cutting. For an invertase switch, place attP, then the promoter of interest upstream, then att. upstream of the gene. When used in an invertase switch, Bxb1 cuts the intervening code and substitutes attL and attR sites at attB and attP, respectively. Bxb1 will not cut attL and attP sites without additional cofactors.
false
false
_848_
0
24144
9
Not in stock
false
Similar sequences already exist on parts registry- this may not be the most condensed sequence available.
false
Richard Fuisz
annotation2429311
1
attB
range2429311
1
1
40
BBa_M36769
1
BBa_M36769
CMV Promoter Reverse Complement
2014-10-23T11:00:00Z
2015-05-08T01:14:06Z
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010611
This is the REVERSE COMPLEMENT of the eukaryotic promoter CMV, for use in invertase switches. CMV is a relatively strong constitutive promoter.
If looking for a CMV Promoter that is faced forward, and for more information on the CMV promoter consult BBa_I712004 http://parts.igem.org/Part:BBa_I712004
false
false
_848_
0
24144
9
Not in stock
false
This is NOT the CMV promoter- it is the reverse complement of a CMV promoter sequence.
false
Richard Fuisz
annotation2429310
1
CMV Promoter
range2429310
1
1
589
BBa_M36689_sequence
1
gtcaagaacgttctagaacgtcaagaacgttctagaacgtcatataaaagcccaggggcaagcggtccggataacggctagcctgaggagctgctgcgtactagatgagagccctggtagtcatccgcctgtcccgcgtcaccgatgctacgacttcaccggagcgtcagctggagtcttgccagcagctctgcgcccagcgcggctgggacgtcgtcggggtagcggaggatctggacgtctccggggcggtcgatccgttcgaccggaagcgcagaccgaacctggcccggtggctagcgttcgaggagcaaccgttcgacgtgatcgtggcgtaccgggtagaccggttgacccgatcgatccggcatctgcaacagctggtccactgggccgaggaccacaagaagctggtcgtctccgcgaccgaagcgcacttcgatacgacgacgccgtttgcggcggtcgtcatcgcgcttatgggaacggtggcgcagatggaattagaagcgatcaaagagcggaaccgttcggctgcgcatttcaatatccgcgccgggaaataccgaggatccctgccgccgtggggatacctgcctacgcgcgtggacggggagtggcggctggtgccggaccctgtgcagcgagagcgcatcctcgaggtgtatcaccgcgtcgtcgacaaccacgagccgctgcacctggtggcccacgacctgaaccggcgtggtgtcctgtcgccgaaggactacttcgcgcagctgcaaggccgcgagccgcagggccgggagtggtcggctaccgcgctgaagcgatcgatgatctccgaggcgatgctcgggtacgcgactctgaacggtaagaccgtccgagacgacgacggagccccgctggtgcgggctgagccgatcctgacccgtgagcagctggaggcgctgcgcgccgagctcgtgaagacctcccgggcgaagcccgcggtgtctaccccgtcgctgctgctgcgggtgttgttctgcgcggtgtgcggggagcccgcgtacaagttcgccgggggaggacgtaagcacccgcgctaccgctgccgctcgatggggttcccgaagcactgcgggaacggcacggtggcgatggccgagtgggacgcgttctgcgaggagcaggtgctggatctgctcggggacgcggagcgtctggagaaagtctgggtagccggctcggactccgcggtcgaactcgcggaggtgaacgcggagctggtggacctgacgtcgctgatcggctccccggcctaccgggccggctctccgcagcgagaagcactggatgcccgtattgcggcgctggccgcgcggcaagaggagctggagggcctagaggctcgcccgtctggctgggagtggcgcgagaccgggcagcggttcggggactggtggcgggagcaggacaccgcggcaaagaacacctggcttcggtcgatgaacgttcggctgacgttcgacgtccgcggcgggctgactcgcacgatcgacttcggggatctgcaagagtacgagcagcatctcaggctcggcagcgtggtcgaacggctacacaccgggatgtcgcctgcaagaaaaagattgaattagtactagagctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatatgcatactagagtttcggatcaagctatgaaggacgcaaagagggaactaaatactagaggatctgacggttcactaaaccagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgcggaactccatatatgggctatgaactaatgaccccgtaattgattactattaataactatactagagttcctcgttttctctcgttggaagaagaagaaacgagaaa
BBa_M36864_sequence
1
ttcctcgttttctctcgttggaagaagaagaaacgagaaa
BBa_M36769_sequence
1
gatctgacggttcactaaaccagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgcggaactccatatatgggctatgaactaatgaccccgtaattgattactattaataacta
BBa_M36670_sequence
1
atgagagccctggtagtcatccgcctgtcccgcgtcaccgatgctacgacttcaccggagcgtcagctggagtcttgccagcagctctgcgcccagcgcggctgggacgtcgtcggggtagcggaggatctggacgtctccggggcggtcgatccgttcgaccggaagcgcagaccgaacctggcccggtggctagcgttcgaggagcaaccgttcgacgtgatcgtggcgtaccgggtagaccggttgacccgatcgatccggcatctgcaacagctggtccactgggccgaggaccacaagaagctggtcgtctccgcgaccgaagcgcacttcgatacgacgacgccgtttgcggcggtcgtcatcgcgcttatgggaacggtggcgcagatggaattagaagcgatcaaagagcggaaccgttcggctgcgcatttcaatatccgcgccgggaaataccgaggatccctgccgccgtggggatacctgcctacgcgcgtggacggggagtggcggctggtgccggaccctgtgcagcgagagcgcatcctcgaggtgtatcaccgcgtcgtcgacaaccacgagccgctgcacctggtggcccacgacctgaaccggcgtggtgtcctgtcgccgaaggactacttcgcgcagctgcaaggccgcgagccgcagggccgggagtggtcggctaccgcgctgaagcgatcgatgatctccgaggcgatgctcgggtacgcgactctgaacggtaagaccgtccgagacgacgacggagccccgctggtgcgggctgagccgatcctgacccgtgagcagctggaggcgctgcgcgccgagctcgtgaagacctcccgggcgaagcccgcggtgtctaccccgtcgctgctgctgcgggtgttgttctgcgcggtgtgcggggagcccgcgtacaagttcgccgggggaggacgtaagcacccgcgctaccgctgccgctcgatggggttcccgaagcactgcgggaacggcacggtggcgatggccgagtgggacgcgttctgcgaggagcaggtgctggatctgctcggggacgcggagcgtctggagaaagtctgggtagccggctcggactccgcggtcgaactcgcggaggtgaacgcggagctggtggacctgacgtcgctgatcggctccccggcctaccgggccggctctccgcagcgagaagcactggatgcccgtattgcggcgctggccgcgcggcaagaggagctggagggcctagaggctcgcccgtctggctgggagtggcgcgagaccgggcagcggttcggggactggtggcgggagcaggacaccgcggcaaagaacacctggcttcggtcgatgaacgttcggctgacgttcgacgtccgcggcgggctgactcgcacgatcgacttcggggatctgcaagagtacgagcagcatctcaggctcggcagcgtggtcgaacggctacacaccgggatgtcgcctgcaagaaaaagattgaattag
BBa_M36765_sequence
1
gtcaagaacgttctagaacgtcaagaacgttctagaacgtcatataaaagcccaggggcaagcggtccggataacggctagcctgaggagctgctgcg
BBa_J52016_sequence
1
ctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatatgca
BBa_M36676_sequence
1
tttcggatcaagctatgaaggacgcaaagagggaactaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z