BBa_M36698
1
BBa_M36698
Pyruvate Sensor v2
2011-05-05T11:00:00Z
2015-05-08T01:14:05Z
See BBa_M36708
Similar to BBa_M36708, this provides a way to test for pyruvate through IclR inhibition of PaceBAK (a portion of the aceBAK promoter).
false
false
_848_
0
9654
9
Not in stock
false
BBa_M36708 had a couple design flaws which have been fixed
false
Wyatt Woodson
component2119666
1
BBa_M36706
component2119662
1
BBa_M36001
component2119667
1
BBa_M36010
component2119680
1
BBa_M36699
component2119661
1
BBa_J23119
annotation2119680
1
BBa_M36699
range2119680
1
990
1207
annotation2119667
1
BBa_M36010
range2119667
1
908
989
annotation2119661
1
BBa_J23119
range2119661
1
1
35
annotation2119666
1
BBa_M36706
range2119666
1
83
907
annotation2119662
1
BBa_M36001
range2119662
1
36
82
BBa_M36706
1
BBa_M36706
Gene encoding IclR
2011-04-25T11:00:00Z
2015-05-08T01:14:06Z
This coding sequence was obtained from genome.jp
IclR represses the aceBAK promoter in E. coli
false
false
_848_
0
9650
9
Not in stock
false
none
false
Katie Lund and Wyatt Woodson
annotation2119104
1
Start Codon
range2119104
1
1
3
annotation2119109
1
Stop Codon
range2119109
1
823
825
annotation2119221
1
IclR Coding Region
range2119221
1
1
825
BBa_M36001
1
BBa_M36001
5' Bicistronic UTR (medium), does not include ATG start
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Vivek Mutalik et al. c/o BIOFAB Emeryville.
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
false
false
_848_
0
52
432
Not in stock
false
Please see long description.
false
Drew Endy
BBa_M36699
1
BBa_M36699
PaceBAK
2011-05-05T11:00:00Z
2015-05-08T01:14:05Z
See BBa_M36707
A portion of the aceBAK promoter region that comprises the promoter as well as the IclR binding sites.
false
false
_848_
0
9654
9
Not in stock
false
This is an updated version of BBa_M36707
false
Wyatt Woodson
annotation2119616
1
-35 Region
range2119616
1
119
127
annotation2119615
1
CRP-cAMP Binding Site
range2119615
1
114
135
annotation2119617
1
-10 Region
range2119617
1
143
150
annotation2119614
1
IclR Box VIII
range2119614
1
126
131
annotation2119606
1
IclR Box I
range2119606
1
11
17
annotation2119609
1
IclR Box IV
range2119609
1
48
54
annotation2119610
1
IclR Box V
range2119610
1
99
105
annotation2119608
1
IclR Box III
range2119608
1
30
36
annotation2119612
1
IclR Box VI
range2119612
1
110
116
annotation2119611
1
IHF Binding Site
range2119611
1
76
89
annotation2119613
1
IclR Box VII
range2119613
1
118
124
annotation2119607
1
IclR Box II
range2119607
1
21
27
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_M36010
1
BBa_M36010
Transcription Terminator (Strong)
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Guillaume Cambray et al. c/o BIOFAB Emeryville
E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
false
true
_848_
0
52
432
Not in stock
false
please see long description
false
Drew Endy
BBa_M36001_sequence
1
tatagagggtattaataatgtatggattaaagggggaggcataacaa
BBa_M36698_sequence
1
ttgacagctagctcagtcctaggtataatgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctgatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatgagtagtcttagttgtgctgaacgaaaagcgcacaacgatccttcgttcacagtggggaagttttcggatccatgacg
BBa_M36010_sequence
1
tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
BBa_M36699_sequence
1
tacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatgagtagtcttagttgtgctgaacgaaaagcgcacaacgatccttcgttcacagtggggaagttttcggatccatgacg
BBa_M36706_sequence
1
atggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctga
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z