LOCUS       BBa_M36701              2178 bp    DNA     linear       22-Dec-2025
DEFINITION  Pyruvate Sensor with Gemini Actuator
ACCESSION   BBa_M36701
VERSION     BBa_M36701.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             36..82
                     /label=BBa_M36001
     misc_feature    83..85
                     /label=Start Codon
     misc_feature    905..907
                     /label=Stop Codon
     CDS             83..907
                     /label=IclR Coding Region
     CDS             83..907
                     /label=BBa_M36706
     terminator      908..989
                     /label=BBa_M36010
     misc_feature    1000..1006
                     /label=IclR Box I
     misc_feature    1010..1016
                     /label=IclR Box II
     misc_feature    1019..1025
                     /label=IclR Box III
     misc_feature    1037..1043
                     /label=IclR Box IV
     misc_feature    1065..1078
                     /label=IHF Binding Site
     misc_feature    1088..1094
                     /label=IclR Box V
     misc_feature    1099..1105
                     /label=IclR Box VI
     misc_feature    1103..1124
                     /label=CRP-cAMP Binding Site
     misc_feature    1107..1113
                     /label=IclR Box VII
     promoter        1108..1116
                     /label=-35 Region
     misc_feature    1115..1120
                     /label=IclR Box VIII
     promoter        990..1131
                     /label=BBa_M36707
     misc_feature    1..1131
                     /label=BBa_M36708
     RBS             1132..1144
                     /label=RBS-3\Weak
     RBS             1138..1141
                     /label=RBS
     misc_feature    1132..1144
                     /label=BBa_B0032
     RBS             1132..1144
                     /label=BBa_B0032
     CDS             1145..1324
                     /label=lacZa
     misc_feature    1325..1381
                     /label=linker
     CDS             1382..2098
                     /label=GFP
     misc_feature    1145..2098
                     /label=BBa_E0051
     stem_loop       2110..2153
                     /label=stem_loop
     misc_feature    2099..2178
                     /label=BBa_B0010
     terminator      2099..2178
                     /label=BBa_B0010
     misc_feature    1132..2178
                     /label=BBa_M36709
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctatag agggtattaa taatgtatgg
       61 attaaagggg gaggcataac aaatggtcgc acccattccc gcgaaacgcg gcagaaaacc
      121 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
      181 actggagtgg attgccgaat ccaatggcag tgtggcactc acggagctgg cgcaacaagc
      241 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
      301 gcgtcaggtt ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
      361 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
      421 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
      481 tattatcgac caggtacagt gtacgcatct gatgcgaatg tccgcgccta tcggcggtaa
      541 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
      601 ggtgacgaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa cgctggtgtc
      661 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
      721 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgtga
      781 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg accgcgtgac
      841 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
      901 gcgctgatcg gtcagtttca cctgatttac gtaaaaaccc gcttcggcgg gtttttgctt
      961 ttggaggggc agaaagatga atgactgtct acctcaggca ccttcgggtg ccttttttat
     1021 ttccgaaaca cacctcagta ggtgaataaa ttttattaat attgttatca ataagttatc
     1081 aagtattttt aattaaaatg gaaattgttt ttgattttga tttttaaatg atcacacagg
     1141 aaagatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1201 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1261 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1321 gcgcgcgggc ggcagcgaag gcggcggcag cgaacatcat catcatcatc atggcagcga
     1381 acgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1441 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     1501 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     1561 cactaccctg acctatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     1621 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     1681 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     1741 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     1801 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     1861 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     1921 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     1981 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     2041 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataacc
     2101 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2161 ttgtcggtga acgctctc
//