BBa_M36707
1
BBa_M36707
IclR binding sites on aceBAK promoter
2011-04-25T11:00:00Z
2015-05-08T01:14:06Z
Genomic sequence coded by genome.jp
IclR inhibits the aceBAK gene in conjunction with pyruvate or glyoxylate by binding these sites on the aceBAK promoter.
false
false
_848_
0
9650
9
Not in stock
false
Only considered IclR binding sites
false
Katie Lund, Wyatt Woodson
annotation2119512
1
IclR Box V
range2119512
1
99
105
annotation2119513
1
IclR Box VI
range2119513
1
110
116
annotation2119509
1
IclR Box III
range2119509
1
30
36
annotation2119515
1
IclR Box VII
range2119515
1
118
124
annotation2119517
1
IclR Box VIII
range2119517
1
126
131
annotation2119508
1
IclR Box II
range2119508
1
21
27
annotation2119514
1
CRP-cAMP Binding Site
range2119514
1
114
135
annotation2119507
1
IclR Box I
range2119507
1
11
17
annotation2119516
1
-35 Region
range2119516
1
119
127
annotation2119510
1
IclR Box IV
range2119510
1
48
54
annotation2119511
1
IHF Binding Site
range2119511
1
76
89
BBa_M36709
1
BBa_M36709
Gemini Protein Generator
2011-05-02T11:00:00Z
2015-05-08T01:14:06Z
Gemini, a Bifunctional Enzymatic and Fluorescent Reporter of Gene Expression. PLOS One. Lance Martin et al.
A medium strength RBS expressing Gemini, ending with a strong terminator.
false
false
_848_
0
9654
9
Not in stock
false
Test Actuator
false
Jeff Quinn and Wyatt Woodson
component2118634
1
BBa_E0051
component2118629
1
BBa_B0032
component2118635
1
BBa_B0010
annotation2118635
1
BBa_B0010
range2118635
1
968
1047
annotation2118634
1
BBa_E0051
range2118634
1
14
967
annotation2118629
1
BBa_B0032
range2118629
1
1
13
BBa_M36701
1
BBa_M36701
Pyruvate Sensor with Gemini Actuator
2011-05-02T11:00:00Z
2015-05-08T01:14:05Z
see individual parts
Constitutive promoter, RBS, IclR gene, terminator, aceBAK promoter, RBS, Gemini, terminator. This is designed so that the IclR gene is synthesized on the same scale as the aceBAK promoter instead of relying on IclR naturally in E. coli.
false
false
_848_
0
9650
9
Not in stock
false
Deciding how much of the IclR binding sites in the aceBAK promoter was necessary, and determining the IclR gene in its functional state.
false
Katie Lund
component2119743
1
BBa_M36709
component2119733
1
BBa_M36708
annotation2119733
1
BBa_M36708
range2119733
1
1
1131
annotation2119743
1
BBa_M36709
range2119743
1
1132
2178
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_M36001
1
BBa_M36001
5' Bicistronic UTR (medium), does not include ATG start
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Vivek Mutalik et al. c/o BIOFAB Emeryville.
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
false
false
_848_
0
52
432
Not in stock
false
Please see long description.
false
Drew Endy
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_M36706
1
BBa_M36706
Gene encoding IclR
2011-04-25T11:00:00Z
2015-05-08T01:14:06Z
This coding sequence was obtained from genome.jp
IclR represses the aceBAK promoter in E. coli
false
false
_848_
0
9650
9
Not in stock
false
none
false
Katie Lund and Wyatt Woodson
annotation2119109
1
Stop Codon
range2119109
1
823
825
annotation2119221
1
IclR Coding Region
range2119221
1
1
825
annotation2119104
1
Start Codon
range2119104
1
1
3
BBa_E0051
1
BBa_E0051
lacZa.GFP fusion
2009-03-01T12:00:00Z
2015-08-31T04:07:25Z
amino acids 3-60 from lacZ and amino acids 2-end of GFP (E0043)
This part is a fusion protein between GFP and the alpha fragment of lacZ. It can be measured via fluorescence from GFP or through an assay for beta-galactosidase in a strain containing the omega fragment of lacZ.
false
false
_41_
0
22
84
Not in stock
false
The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc.
false
Austin Che
annotation2001726
1
lacZa
range2001726
1
1
180
annotation2001727
1
linker
range2001727
1
181
237
annotation2001728
1
GFP
range2001728
1
238
954
BBa_M36708
1
BBa_M36708
Pyruvate Sensor
2011-04-25T11:00:00Z
2015-05-08T01:14:06Z
Katie Lund and Wyatt Woodson
parts obtained from Stanford BIOE44 - S11
IclR binds with pyruvate to form a more stable structure to bind to the inhibitory regulation sites on the aceBAK promoter. By combining the IclR gene and the aceBAK promoter the presence of IclR and pyruvate will inhibit the aceBAK promoter and any genes downstream of it. While there will be some residual transcription in the presence of only IclR, the presence of pyruvate increases the stability of binding with the aceBAK promoter region so inhibitory activities are higher.
false
false
_848_
0
9650
9
Not in stock
false
a lot
false
Katie Lund and Wyatt Woodson
component2119525
1
BBa_M36010
component2119520
1
BBa_M36001
component2119519
1
BBa_J23119
component2119537
1
BBa_M36707
component2119524
1
BBa_M36706
annotation2119519
1
BBa_J23119
range2119519
1
1
35
annotation2119537
1
BBa_M36707
range2119537
1
990
1131
annotation2119524
1
BBa_M36706
range2119524
1
83
907
annotation2119520
1
BBa_M36001
range2119520
1
36
82
annotation2119525
1
BBa_M36010
range2119525
1
908
989
BBa_M36010
1
BBa_M36010
Transcription Terminator (Strong)
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Guillaume Cambray et al. c/o BIOFAB Emeryville
E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
false
true
_848_
0
52
432
Not in stock
false
please see long description
false
Drew Endy
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_M36001_sequence
1
tatagagggtattaataatgtatggattaaagggggaggcataacaa
BBa_M36709_sequence
1
tcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_M36708_sequence
1
ttgacagctagctcagtcctaggtataatgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctgatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatga
BBa_E0051_sequence
1
atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_B0032_sequence
1
tcacacaggaaag
BBa_M36010_sequence
1
tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
BBa_M36706_sequence
1
atggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctga
BBa_M36701_sequence
1
ttgacagctagctcagtcctaggtataatgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctgatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatgatcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_M36707_sequence
1
tacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z