BBa_M36701
type: ComponentDefinition
persistent identity: BBa M36701
display id: BBa_M36701
version: 1
was generated by: iGEM to SBOL conversion
description: Pyruvate Sensor with Gemini Actuator
created: 2011-05-02T11:00:00Z
modified: 2015-05-08T01:14:05Z
mutable provenance: see individual parts
owned by: james
owned by: myers
mutable description: Constitutive promoter, RBS, IclR gene, terminator, aceBAK promoter, RBS, Gemini, terminator. This is designed so that the IclR gene is synthesized on the same scale as the aceBAK promoter instead of relying on IclR naturally in E. coli.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Deciding how much of the IclR binding sites in the aceBAK promoter was necessary, and determining the IclR gene in its functional state.
star: false
creator: Katie Lund
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36709
component: BBa_M36708
sequence annotation: BBa_M36708
sequence annotation: BBa_M36709
sequence: https://synbiohub.org/public/igem/BBa_M36701_sequence/1

BBa_M36709
type: Component
persistent identity: component2119743
display id: component2119743
version: 1
top level: BBa_M36701
definition: BBa_M36709
access: public

BBa_M36708
type: Component
persistent identity: component2119733
display id: component2119733
version: 1
top level: BBa_M36701
definition: BBa_M36708
access: public

BBa_M36708
type: SequenceAnnotation
persistent identity: annotation2119733
display id: annotation2119733
version: 1
top level: BBa_M36701
location: https://synbiohub.org/public/igem/BBa_M36701/annotation2119733/range2119733/1
component: BBa_M36708

https://synbiohub.org/public/igem/BBa_M36701/annotation2119733/range2119733/1
type: Range
persistent identity: range2119733
display id: range2119733
version: 1
top level: BBa_M36701
direction: direction/reverse
start: 1
end: 1131
orientation: inline

BBa_M36709
type: SequenceAnnotation
persistent identity: annotation2119743
display id: annotation2119743
version: 1
top level: BBa_M36701
location: https://synbiohub.org/public/igem/BBa_M36701/annotation2119743/range2119743/1
component: BBa_M36709

https://synbiohub.org/public/igem/BBa_M36701/annotation2119743/range2119743/1
type: Range
persistent identity: range2119743
display id: range2119743
version: 1
top level: BBa_M36701
direction: direction/reverse
start: 1132
end: 2178
orientation: inline

BBa_M36708
type: ComponentDefinition
persistent identity: BBa M36708
display id: BBa_M36708
version: 1
was generated by: iGEM to SBOL conversion
description: Pyruvate Sensor
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: Katie Lund and Wyatt Woodson parts obtained from Stanford BIOE44 - S11
owned by: james
owned by: myers
mutable description: IclR binds with pyruvate to form a more stable structure to bind to the inhibitory regulation sites on the aceBAK promoter. By combining the IclR gene and the aceBAK promoter the presence of IclR and pyruvate will inhibit the aceBAK promoter and any genes downstream of it. While there will be some residual transcription in the presence of only IclR, the presence of pyruvate increases the stability of binding with the aceBAK promoter region so inhibitory activities are higher.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: a lot
star: false
creator: Katie Lund and Wyatt Woodson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M36706
component: BBa_M36001
component: BBa_J23119
component: BBa_M36707
component: BBa_M36010
sequence annotation: BBa_J23119
sequence annotation: BBa_M36001
sequence annotation: BBa_M36706
sequence annotation: BBa_M36010
sequence annotation: BBa_M36707
sequence: https://synbiohub.org/public/igem/BBa_M36708_sequence/1

BBa_M36706
type: Component
persistent identity: component2119524
display id: component2119524
version: 1
top level: BBa_M36708
definition: BBa_M36706
access: public

BBa_M36001
type: Component
persistent identity: component2119520
display id: component2119520
version: 1
top level: BBa_M36708
definition: BBa_M36001
access: public

BBa_J23119
type: Component
persistent identity: component2119519
display id: component2119519
version: 1
top level: BBa_M36708
definition: BBa_J23119
access: public

BBa_M36707
type: Component
persistent identity: component2119537
display id: component2119537
version: 1
top level: BBa_M36708
definition: BBa_M36707
access: public

BBa_M36010
type: Component
persistent identity: component2119525
display id: component2119525
version: 1
top level: BBa_M36708
definition: BBa_M36010
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2119519
display id: annotation2119519
version: 1
top level: BBa_M36708
location: https://synbiohub.org/public/igem/BBa_M36708/annotation2119519/range2119519/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_M36708/annotation2119519/range2119519/1
type: Range
persistent identity: range2119519
display id: range2119519
version: 1
top level: BBa_M36708
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_M36001
type: SequenceAnnotation
persistent identity: annotation2119520
display id: annotation2119520
version: 1
top level: BBa_M36708
location: https://synbiohub.org/public/igem/BBa_M36708/annotation2119520/range2119520/1
component: BBa_M36001

https://synbiohub.org/public/igem/BBa_M36708/annotation2119520/range2119520/1
type: Range
persistent identity: range2119520
display id: range2119520
version: 1
top level: BBa_M36708
direction: direction/reverse
start: 36
end: 82
orientation: inline

BBa_M36706
type: SequenceAnnotation
persistent identity: annotation2119524
display id: annotation2119524
version: 1
top level: BBa_M36708
location: https://synbiohub.org/public/igem/BBa_M36708/annotation2119524/range2119524/1
component: BBa_M36706

https://synbiohub.org/public/igem/BBa_M36708/annotation2119524/range2119524/1
type: Range
persistent identity: range2119524
display id: range2119524
version: 1
top level: BBa_M36708
direction: direction/reverse
start: 83
end: 907
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2119525
display id: annotation2119525
version: 1
top level: BBa_M36708
location: https://synbiohub.org/public/igem/BBa_M36708/annotation2119525/range2119525/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36708/annotation2119525/range2119525/1
type: Range
persistent identity: range2119525
display id: range2119525
version: 1
top level: BBa_M36708
direction: direction/reverse
start: 908
end: 989
orientation: inline

BBa_M36707
type: SequenceAnnotation
persistent identity: annotation2119537
display id: annotation2119537
version: 1
top level: BBa_M36708
location: https://synbiohub.org/public/igem/BBa_M36708/annotation2119537/range2119537/1
component: BBa_M36707

https://synbiohub.org/public/igem/BBa_M36708/annotation2119537/range2119537/1
type: Range
persistent identity: range2119537
display id: range2119537
version: 1
top level: BBa_M36708
direction: direction/reverse
start: 990
end: 1131
orientation: inline

BBa_M36709
type: ComponentDefinition
persistent identity: BBa M36709
display id: BBa_M36709
version: 1
was generated by: iGEM to SBOL conversion
description: Gemini Protein Generator
created: 2011-05-02T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: Gemini, a Bifunctional Enzymatic and Fluorescent Reporter of Gene Expression. PLOS One. Lance Martin et al.
owned by: james
owned by: myers
mutable description: A medium strength RBS expressing Gemini, ending with a strong terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9654
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Test Actuator
star: false
creator: Jeff Quinn and Wyatt Woodson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_E0051
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_E0051
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_M36709_sequence/1

BBa_B0010
type: Component
persistent identity: component2118635
display id: component2118635
version: 1
top level: BBa_M36709
definition: BBa_B0010
access: public

BBa_E0051
type: Component
persistent identity: component2118634
display id: component2118634
version: 1
top level: BBa_M36709
definition: BBa_E0051
access: public

BBa_B0032
type: Component
persistent identity: component2118629
display id: component2118629
version: 1
top level: BBa_M36709
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2118629
display id: annotation2118629
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118629/range2118629/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_M36709/annotation2118629/range2118629/1
type: Range
persistent identity: range2118629
display id: range2118629
version: 1
top level: BBa_M36709
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0051
type: SequenceAnnotation
persistent identity: annotation2118634
display id: annotation2118634
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118634/range2118634/1
component: BBa_E0051

https://synbiohub.org/public/igem/BBa_M36709/annotation2118634/range2118634/1
type: Range
persistent identity: range2118634
display id: range2118634
version: 1
top level: BBa_M36709
direction: direction/reverse
start: 14
end: 967
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2118635
display id: annotation2118635
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118635/range2118635/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M36709/annotation2118635/range2118635/1
type: Range
persistent identity: range2118635
display id: range2118635
version: 1
top level: BBa_M36709
direction: direction/forward
start: 968
end: 1047
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_M36001
type: ComponentDefinition
persistent identity: BBa M36001
display id: BBa_M36001
version: 1
was generated by: iGEM to SBOL conversion
description: 5' Bicistronic UTR (medium), does not include ATG start
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Vivek Mutalik et al. c/o BIOFAB Emeryville.
owned by: james
owned by: myers
mutable description: 5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 52
owning group id: 432
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Please see long description.
star: false
creator: Drew Endy
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M36001_sequence/1

BBa_M36010
type: ComponentDefinition
persistent identity: BBa M36010
display id: BBa_M36010
version: 1
was generated by: iGEM to SBOL conversion
description: Transcription Terminator (Strong)
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Guillaume Cambray et al. c/o BIOFAB Emeryville
owned by: james
owned by: myers
mutable description: E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
discontinued: false
dominant: true
group u list: _848_
m user id: 0
owner id: 52
owning group id: 432
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: please see long description
star: false
creator: Drew Endy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_M36010_sequence/1

BBa_M36706
type: ComponentDefinition
persistent identity: BBa M36706
display id: BBa_M36706
version: 1
was generated by: iGEM to SBOL conversion
description: Gene encoding IclR
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: This coding sequence was obtained from genome.jp
owned by: james
owned by: myers
mutable description: IclR represses the aceBAK promoter in E. coli
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Katie Lund and Wyatt Woodson
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: IclR Coding Region
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M36706_sequence/1

IclR Coding Region
type: SequenceAnnotation
persistent identity: annotation2119221
display id: annotation2119221
version: 1
top level: BBa_M36706
location: https://synbiohub.org/public/igem/BBa_M36706/annotation2119221/range2119221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M36706/annotation2119221/range2119221/1
type: Range
persistent identity: range2119221
display id: range2119221
version: 1
top level: BBa_M36706
direction: direction/forward
start: 1
end: 825
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2119104
display id: annotation2119104
version: 1
top level: BBa_M36706
location: https://synbiohub.org/public/igem/BBa_M36706/annotation2119104/range2119104/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M36706/annotation2119104/range2119104/1
type: Range
persistent identity: range2119104
display id: range2119104
version: 1
top level: BBa_M36706
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2119109
display id: annotation2119109
version: 1
top level: BBa_M36706
location: https://synbiohub.org/public/igem/BBa_M36706/annotation2119109/range2119109/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M36706/annotation2119109/range2119109/1
type: Range
persistent identity: range2119109
display id: range2119109
version: 1
top level: BBa_M36706
direction: direction/forward
start: 823
end: 825
orientation: inline

BBa_M36707
type: ComponentDefinition
persistent identity: BBa M36707
display id: BBa_M36707
version: 1
was generated by: iGEM to SBOL conversion
description: IclR binding sites on aceBAK promoter
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: Genomic sequence coded by genome.jp
owned by: james
owned by: myers
mutable description: IclR inhibits the aceBAK gene in conjunction with pyruvate or glyoxylate by binding these sites on the aceBAK promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Only considered IclR binding sites
star: false
creator: Katie Lund, Wyatt Woodson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: IclR Box I
sequence annotation: IclR Box II
sequence annotation: IclR Box III
sequence annotation: IclR Box IV
sequence annotation: IHF Binding Site
sequence annotation: IclR Box V
sequence annotation: IclR Box VI
sequence annotation: CRP-cAMP Binding Site
sequence annotation: IclR Box VII
sequence annotation: -35 Region
sequence annotation: IclR Box VIII
sequence: https://synbiohub.org/public/igem/BBa_M36707_sequence/1

IclR Box I
type: SequenceAnnotation
persistent identity: annotation2119507
display id: annotation2119507
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119507/range2119507/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36707/annotation2119507/range2119507/1
type: Range
persistent identity: range2119507
display id: range2119507
version: 1
top level: BBa_M36707
direction: direction/forward
start: 11
end: 17
orientation: inline

IclR Box II
type: SequenceAnnotation
persistent identity: annotation2119508
display id: annotation2119508
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119508/range2119508/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36707/annotation2119508/range2119508/1
type: Range
persistent identity: range2119508
display id: range2119508
version: 1
top level: BBa_M36707
direction: direction/forward
start: 21
end: 27
orientation: inline

IclR Box III
type: SequenceAnnotation
persistent identity: annotation2119509
display id: annotation2119509
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119509/range2119509/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36707/annotation2119509/range2119509/1
type: Range
persistent identity: range2119509
display id: range2119509
version: 1
top level: BBa_M36707
direction: direction/forward
start: 30
end: 36
orientation: inline

IclR Box IV
type: SequenceAnnotation
persistent identity: annotation2119510
display id: annotation2119510
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119510/range2119510/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36707/annotation2119510/range2119510/1
type: Range
persistent identity: range2119510
display id: range2119510
version: 1
top level: BBa_M36707
direction: direction/forward
start: 48
end: 54
orientation: inline

IHF Binding Site
type: SequenceAnnotation
persistent identity: annotation2119511
display id: annotation2119511
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119511/range2119511/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36707/annotation2119511/range2119511/1
type: Range
persistent identity: range2119511
display id: range2119511
version: 1
top level: BBa_M36707
direction: direction/forward
start: 76
end: 89
orientation: inline

IclR Box V
type: SequenceAnnotation
persistent identity: annotation2119512
display id: annotation2119512
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119512/range2119512/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_M36707/annotation2119512/range2119512/1
type: Range
persistent identity: range2119512
display id: range2119512
version: 1
top level: BBa_M36707
direction: direction/forward
start: 99
end: 105
orientation: inline

IclR Box VI
type: SequenceAnnotation
persistent identity: annotation2119513
display id: annotation2119513
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119513/range2119513/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_M36707/annotation2119513/range2119513/1
type: Range
persistent identity: range2119513
display id: range2119513
version: 1
top level: BBa_M36707
direction: direction/forward
start: 110
end: 116
orientation: inline

CRP-cAMP Binding Site
type: SequenceAnnotation
persistent identity: annotation2119514
display id: annotation2119514
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119514/range2119514/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36707/annotation2119514/range2119514/1
type: Range
persistent identity: range2119514
display id: range2119514
version: 1
top level: BBa_M36707
direction: direction/forward
start: 114
end: 135
orientation: inline

IclR Box VII
type: SequenceAnnotation
persistent identity: annotation2119515
display id: annotation2119515
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119515/range2119515/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36707/annotation2119515/range2119515/1
type: Range
persistent identity: range2119515
display id: range2119515
version: 1
top level: BBa_M36707
direction: direction/forward
start: 118
end: 124
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation2119516
display id: annotation2119516
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119516/range2119516/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M36707/annotation2119516/range2119516/1
type: Range
persistent identity: range2119516
display id: range2119516
version: 1
top level: BBa_M36707
direction: direction/forward
start: 119
end: 127
orientation: inline

IclR Box VIII
type: SequenceAnnotation
persistent identity: annotation2119517
display id: annotation2119517
version: 1
top level: BBa_M36707
location: https://synbiohub.org/public/igem/BBa_M36707/annotation2119517/range2119517/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36707/annotation2119517/range2119517/1
type: Range
persistent identity: range2119517
display id: range2119517
version: 1
top level: BBa_M36707
direction: direction/forward
start: 126
end: 131
orientation: inline

BBa_E0051
type: ComponentDefinition
persistent identity: BBa E0051
display id: BBa_E0051
version: 1
was generated by: iGEM to SBOL conversion
description: lacZa.GFP fusion
created: 2009-03-01T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: amino acids 3-60 from lacZ and amino acids 2-end of GFP (E0043)
owned by: james
owned by: myers
mutable description: This part is a fusion protein between GFP and the alpha fragment of lacZ. It can be measured via fluorescence from GFP or through an assay for beta-galactosidase in a strain containing the omega fragment of lacZ.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 22
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc.
star: false
creator: Austin Che
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: lacZa
sequence annotation: linker
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_E0051_sequence/1

lacZa
type: SequenceAnnotation
persistent identity: annotation2001726
display id: annotation2001726
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001726/range2001726/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0051/annotation2001726/range2001726/1
type: Range
persistent identity: range2001726
display id: range2001726
version: 1
top level: BBa_E0051
direction: direction/forward
start: 1
end: 180
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2001727
display id: annotation2001727
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001727/range2001727/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E0051/annotation2001727/range2001727/1
type: Range
persistent identity: range2001727
display id: range2001727
version: 1
top level: BBa_E0051
direction: direction/forward
start: 181
end: 237
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2001728
display id: annotation2001728
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001728/range2001728/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0051/annotation2001728/range2001728/1
type: Range
persistent identity: range2001728
display id: range2001728
version: 1
top level: BBa_E0051
direction: direction/forward
start: 238
end: 954
orientation: inline

https://synbiohub.org/public/igem/BBa_M36701_sequence/1
type: Sequence
persistent identity: BBa M36701 sequence
display id: BBa_M36701_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctgatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatgatcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgcccttt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36708_sequence/1
type: Sequence
persistent identity: BBa M36708 sequence
display id: BBa_M36708_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctgatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36709_sequence/1
type: Sequence
persistent identity: BBa M36709 sequence
display id: BBa_M36709_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36001_sequence/1
type: Sequence
persistent identity: BBa M36001 sequence
display id: BBa_M36001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatagagggtattaataatgtatggattaaagggggaggcataacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36010_sequence/1
type: Sequence
persistent identity: BBa M36010 sequence
display id: BBa_M36010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36706_sequence/1
type: Sequence
persistent identity: BBa M36706 sequence
display id: BBa_M36706_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36707_sequence/1
type: Sequence
persistent identity: BBa M36707 sequence
display id: BBa_M36707_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0051_sequence/1
type: Sequence
persistent identity: BBa E0051 sequence
display id: BBa_E0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z