LOCUS       BBa_M36704              1265 bp    DNA     linear       11-Dec-2025
DEFINITION  PaceBAK with Gemini
ACCESSION   BBa_M36704
VERSION     BBa_M36704.1  
FEATURES             Location/Qualifiers
     misc_feature    11..17
                     /label=IclR Box I
     misc_feature    21..27
                     /label=IclR Box II
     misc_feature    30..36
                     /label=IclR Box III
     misc_feature    48..54
                     /label=IclR Box IV
     misc_feature    99..105
                     /label=IclR Box V
     misc_feature    76..89
                     /label=IHF Binding Site
     misc_feature    110..116
                     /label=IclR Box VI
     misc_feature    118..124
                     /label=IclR Box VII
     misc_feature    126..131
                     /label=IclR Box VIII
     misc_feature    114..135
                     /label=CRP-cAMP Binding Site
     promoter        119..127
                     /label=-35 Region
     promoter        143..150
                     /label=-10 Region
     promoter        1..218
                     /label=BBa_M36699
     RBS             219..231
                     /label=RBS-3\Weak
     RBS             225..228
                     /label=RBS
     misc_feature    219..231
                     /label=BBa_B0032
     RBS             219..231
                     /label=BBa_B0032
     CDS             232..411
                     /label=lacZa
     misc_feature    412..468
                     /label=linker
     CDS             469..1185
                     /label=GFP
     misc_feature    232..1185
                     /label=BBa_E0051
     stem_loop       1197..1240
                     /label=stem_loop
     misc_feature    1186..1265
                     /label=BBa_B0010
     terminator      1186..1265
                     /label=BBa_B0010
     misc_feature    219..1265
                     /label=BBa_M36709
ORIGIN
        1 tacctcaggc accttcgggt gcctttttta tttccgaaac acacctcagt aggtgaataa
       61 attttattaa tattgttatc aataagttat caagtatttt taattaaaat ggaaattgtt
      121 tttgattttg atttttaaat gagtagtctt agttgtgctg aacgaaaagc gcacaacgat
      181 ccttcgttca cagtggggaa gttttcggat ccatgacgtc acacaggaaa gatgaccatg
      241 attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      301 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      361 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgcgggcggc
      421 agcgaaggcg gcggcagcga acatcatcat catcatcatg gcagcgaacg taaaggagaa
      481 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      541 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      601 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac taccctgacc
      661 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
      721 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
      781 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
      841 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
      901 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
      961 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     1021 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     1081 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     1141 gctgctggga ttacacatgg catggatgaa ctatacaaat aataaccagg catcaaataa
     1201 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1261 ctctc
//