BBa_M36001
1
BBa_M36001
5' Bicistronic UTR (medium), does not include ATG start
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Vivek Mutalik et al. c/o BIOFAB Emeryville.
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
false
false
_848_
0
52
432
Not in stock
false
Please see long description.
false
Drew Endy
BBa_M36706
1
BBa_M36706
Gene encoding IclR
2011-04-25T11:00:00Z
2015-05-08T01:14:06Z
This coding sequence was obtained from genome.jp
IclR represses the aceBAK promoter in E. coli
false
false
_848_
0
9650
9
Not in stock
false
none
false
Katie Lund and Wyatt Woodson
annotation2119104
1
Start Codon
range2119104
1
1
3
annotation2119109
1
Stop Codon
range2119109
1
823
825
annotation2119221
1
IclR Coding Region
range2119221
1
1
825
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_M36708
1
BBa_M36708
Pyruvate Sensor
2011-04-25T11:00:00Z
2015-05-08T01:14:06Z
Katie Lund and Wyatt Woodson
parts obtained from Stanford BIOE44 - S11
IclR binds with pyruvate to form a more stable structure to bind to the inhibitory regulation sites on the aceBAK promoter. By combining the IclR gene and the aceBAK promoter the presence of IclR and pyruvate will inhibit the aceBAK promoter and any genes downstream of it. While there will be some residual transcription in the presence of only IclR, the presence of pyruvate increases the stability of binding with the aceBAK promoter region so inhibitory activities are higher.
false
false
_848_
0
9650
9
Not in stock
false
a lot
false
Katie Lund and Wyatt Woodson
component2119519
1
BBa_J23119
component2119537
1
BBa_M36707
component2119520
1
BBa_M36001
component2119525
1
BBa_M36010
component2119524
1
BBa_M36706
annotation2119519
1
BBa_J23119
range2119519
1
1
35
annotation2119525
1
BBa_M36010
range2119525
1
908
989
annotation2119520
1
BBa_M36001
range2119520
1
36
82
annotation2119537
1
BBa_M36707
range2119537
1
990
1131
annotation2119524
1
BBa_M36706
range2119524
1
83
907
BBa_M36707
1
BBa_M36707
IclR binding sites on aceBAK promoter
2011-04-25T11:00:00Z
2015-05-08T01:14:06Z
Genomic sequence coded by genome.jp
IclR inhibits the aceBAK gene in conjunction with pyruvate or glyoxylate by binding these sites on the aceBAK promoter.
false
false
_848_
0
9650
9
Not in stock
false
Only considered IclR binding sites
false
Katie Lund, Wyatt Woodson
annotation2119513
1
IclR Box VI
range2119513
1
110
116
annotation2119510
1
IclR Box IV
range2119510
1
48
54
annotation2119517
1
IclR Box VIII
range2119517
1
126
131
annotation2119507
1
IclR Box I
range2119507
1
11
17
annotation2119508
1
IclR Box II
range2119508
1
21
27
annotation2119516
1
-35 Region
range2119516
1
119
127
annotation2119515
1
IclR Box VII
range2119515
1
118
124
annotation2119511
1
IHF Binding Site
range2119511
1
76
89
annotation2119509
1
IclR Box III
range2119509
1
30
36
annotation2119512
1
IclR Box V
range2119512
1
99
105
annotation2119514
1
CRP-cAMP Binding Site
range2119514
1
114
135
BBa_M36010
1
BBa_M36010
Transcription Terminator (Strong)
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Guillaume Cambray et al. c/o BIOFAB Emeryville
E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
false
true
_848_
0
52
432
Not in stock
false
please see long description
false
Drew Endy
BBa_M36001_sequence
1
tatagagggtattaataatgtatggattaaagggggaggcataacaa
BBa_M36708_sequence
1
ttgacagctagctcagtcctaggtataatgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctgatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatga
BBa_M36010_sequence
1
tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
BBa_M36706_sequence
1
atggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctga
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_M36707_sequence
1
tacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z