medium-chain alkane hydroxylating device
alkB2, rubA1, rubA2 are from Pseudomonas aeruginosa PAO1. rubB is from Pseudomonas fluorescens Pf-5.
This system is design to hydroxylate medium-length alkanes (C5-C12). alkB1 codes for an alkane hydroxylating protein. rubA and rubB code for two other proteins to supply reducing electrons. Alkanols derived from this reaction are intermediates as plasticizers and solvents in chemical and pharmaceutical industries.
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
5' Bicistronic UTR (strong), does not include ATG start
c/o Vivek Mutalik et al. BIOFAB Emeryville
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
alkB1 codes for an alkane hydroxylating protein.
rubA codes for a protein that supplies reducing electrons to an alkane hydroxylating reaction.
rubA2 codes for a protein that supplies reducing electrons for an alkane hydroxylating reaction.
rubB catalyzes the conversion of rubredoxin from reduced to oxidized state.
Guillaume Cambray et al. c/o BIOFAB Emeryville
E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
Conversion of the iGEM parts registry to SBOL2.1