short-chain alkane hydroxylating device (mmoXYBZDC)
The mmoXYBZDC gene cluster is a methane monooxygenase found in methylococcus capsulatus. It catalyzes a C-H bond in methane, converting it to methanol.
The whole mmoXYBZDC gene cluster needs to work together to create the desired methane monooxygenase function.
5' Bicistronic UTR (strong), does not include ATG start
c/o Vivek Mutalik et al. BIOFAB Emeryville
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
mmoX (methane monooxygenase subunit A, alpha chain)
From Methylococcus capsulatus (Bath) http://www.ncbi.nlm.nih.gov/gene/3104807
mmoX is part of a methane monooxygenase complex (subunit A, alpha chain). It helps oxidize a C-H bond in methane, converting it to methanol.
mmoX needs the other mmo genes for the methane monooxygenase function.
mmoY (methane monooxygenase subunit A, beta chain)
From Methylococcus capsulatus (Bath) http://www.ncbi.nlm.nih.gov/gene?term=mmoy
mmoY is part of a methane monooxygenase complex (subunit A, alpha chain). It helps oxidize a C-H bond in methane, converting it to methanol.
mmoY needs to work with the gene cluster mmoXYBZDC to create the desired methane monooxygenase function.
Guillaume Cambray et al. c/o BIOFAB Emeryville
E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
mmoB is part of a methane monooxygenase complex (subunit B). It helps oxidize a C-H bond in methane, converting it to methanol.
mmoB needs to work with the gene cluster mmoXYBZDC to create the desired methane monooxygenase function.
mmoZ (methane monooxygenase subunit A, gamma chain)
mmoZ is part of a methane monooxygenase complex (subunit A, gamma chain). It helps oxidize a C-H bond in methane, converting it to methanol.
mmoZ needs to work with gene cluster mmoXYBZDC to create the desired methane monooxygenase function.
From Methylococcus capsulatus str. Bath http://www.ncbi.nlm.nih.gov/gene?term=mmod
mmoD is an open reading frame (orfY) in a methane monooxygenase complex (subunit D). It helps oxidize a C-H bond in methane, converting it to methanol.
mmoD needs to work with the gene cluster mmoXYBZDC to create the desired methane monooxygenase function.
mmoC is part of a methane monooxygenase complex (subunit C). It helps oxidize a C-H bond in methane, converting it to methanol.
mmoC needs to work with the gene cluster mmoXYBZDC to create the desired methane monooxygenase function.
Conversion of the iGEM parts registry to SBOL2.1