LOCUS       BBa_M36731              1124 bp    DNA     linear       14-Jun-2024
DEFINITION  EfeU elemental iron (II) transporter + 5' UTR (medium) + 
            terminator + Fur promoter
ACCESSION   BBa_M36731
VERSION     BBa_M36731.1  
FEATURES             Location/Qualifiers
     RBS             1..47
                     /label=BBa_M36001
     misc_feature    48..50
                     /label=Start codon
     misc_feature    75..89
                     /label=REGLE motif
     misc_feature    432..446
                     /label=REGLE motif
     misc_feature    876..878
                     /label=Stop codon
     misc_feature    153..153
                     /label=Repaired frameshift (see notes)
     CDS             48..878
                     /label=BBa_M36042
     terminator      879..960
                     /label=BBa_M36010
     misc_feature    1..960
                     /label=BBa_M36729
     promoter        1042..1047
                     /label=-35
     promoter        1063..1068
                     /label=-10
     misc_feature    1072..1072
                     /label=start
     misc_feature    1119..1124
                     /label=SpeI
     misc_feature    1062..1080
                     /label=Possible repressor region
     misc_feature    1055..1060
                     /label=SalI
     promoter        961..1124
                     /label=BBa_M36011
ORIGIN
        1 tatagagggt attaataatg tatggattaa agggggaggc ataacaaatg tttgttccgt
       61 ttctcattat gttgcgcgaa ggacttgaag ccgcgctgat tgtcagtttg attgccagct
      121 atcttaagcg tacccagcga ggccgatgga ttggtgtgat gtggattggc gtgttgcttg
      181 ccgctgcgtt gtgcctgggc ttgggtatct tcattaacga aaccaccggc gaatttccgc
      241 aaaaagaaca ggaactgttt gaaggtatcg tggcggtgat cgccgtggtg atccttacct
      301 ggatggtttt ctggatgcgc aaagtgtcgc gcaacgtcaa agtgcaactg gaacaggcag
      361 tcgatagcgc attgcagcgt ggaaatcatc atggctgggc gctggtgatg atggtctttt
      421 ttgccgttgc aagggaaggg ctggagtcgg tctttttcct gctggcggca tttcaacaag
      481 atgtcgggat ctggccgccg ctgggtgcaa tgctcggtct tgctactgcc gtggtgctag
      541 gcttcctgct ctactggggc ggtattcgcc tcaatcttgg tgcatttttt aaatggacca
      601 gcctgtttat tctcttcgtc gccgcagggc tggcagctgg tgccattcgc gcatttcatg
      661 aagccggatt gtggaaccac tttcaggaaa tcgccttcga tatgagtgcg gtgctctcaa
      721 ctcactcgct gtttggcacg ctgatggaag ggatttttgg ctatcaggaa gcgccgagcg
      781 tcagcgaagt cgccgtctgg tttatttatc tcatcccggc gctggtggca tttgctctgc
      841 caccacgcgc aggggcgaca gcgtctcgct ccgcgtaatc ggtcagtttc acctgattta
      901 cgtaaaaacc cgcttcggcg ggtttttgct tttggagggg cagaaagatg aatgactgtc
      961 gactgcaaag ccggcgctcc ggtgaacgct tcacggccaa tcgctgtgca tccggccaca
     1021 gttgacggga atcaccccct gtggacttcc tcaggtcgac attaggttag gctcacctaa
     1081 gttcatcgcc ccctgtggtg ctcaccccca ggagcatcac tagt
//