LOCUS       BBa_M36732              2171 bp    DNA     linear       14-Jun-2024
DEFINITION  EfeU iron (II) transporter + 5' UTR (medium) + terminator + Fur 
            promoter + Gemini
ACCESSION   BBa_M36732
VERSION     BBa_M36732.1  
FEATURES             Location/Qualifiers
     RBS             1..47
                     /label=BBa_M36001
     misc_feature    48..50
                     /label=Start codon
     misc_feature    75..89
                     /label=REGLE motif
     misc_feature    432..446
                     /label=REGLE motif
     misc_feature    876..878
                     /label=Stop codon
     misc_feature    153..153
                     /label=Repaired frameshift (see notes)
     CDS             48..878
                     /label=BBa_M36042
     terminator      879..960
                     /label=BBa_M36010
     misc_feature    1..960
                     /label=BBa_M36729
     promoter        1042..1047
                     /label=-35
     promoter        1063..1068
                     /label=-10
     misc_feature    1072..1072
                     /label=start
     misc_feature    1119..1124
                     /label=SpeI
     misc_feature    1062..1080
                     /label=Possible repressor region
     misc_feature    1055..1060
                     /label=SalI
     promoter        961..1124
                     /label=BBa_M36011
     misc_feature    1..1124
                     /label=BBa_M36731
     RBS             1125..1137
                     /label=RBS-3\Weak
     RBS             1131..1134
                     /label=RBS
     misc_feature    1125..1137
                     /label=BBa_B0032
     RBS             1125..1137
                     /label=BBa_B0032
     CDS             1138..1317
                     /label=lacZa
     misc_feature    1318..1374
                     /label=linker
     CDS             1375..2091
                     /label=GFP
     misc_feature    1138..2091
                     /label=BBa_E0051
     stem_loop       2103..2146
                     /label=stem_loop
     misc_feature    2092..2171
                     /label=BBa_B0010
     terminator      2092..2171
                     /label=BBa_B0010
     misc_feature    1125..2171
                     /label=BBa_M36709
ORIGIN
        1 tatagagggt attaataatg tatggattaa agggggaggc ataacaaatg tttgttccgt
       61 ttctcattat gttgcgcgaa ggacttgaag ccgcgctgat tgtcagtttg attgccagct
      121 atcttaagcg tacccagcga ggccgatgga ttggtgtgat gtggattggc gtgttgcttg
      181 ccgctgcgtt gtgcctgggc ttgggtatct tcattaacga aaccaccggc gaatttccgc
      241 aaaaagaaca ggaactgttt gaaggtatcg tggcggtgat cgccgtggtg atccttacct
      301 ggatggtttt ctggatgcgc aaagtgtcgc gcaacgtcaa agtgcaactg gaacaggcag
      361 tcgatagcgc attgcagcgt ggaaatcatc atggctgggc gctggtgatg atggtctttt
      421 ttgccgttgc aagggaaggg ctggagtcgg tctttttcct gctggcggca tttcaacaag
      481 atgtcgggat ctggccgccg ctgggtgcaa tgctcggtct tgctactgcc gtggtgctag
      541 gcttcctgct ctactggggc ggtattcgcc tcaatcttgg tgcatttttt aaatggacca
      601 gcctgtttat tctcttcgtc gccgcagggc tggcagctgg tgccattcgc gcatttcatg
      661 aagccggatt gtggaaccac tttcaggaaa tcgccttcga tatgagtgcg gtgctctcaa
      721 ctcactcgct gtttggcacg ctgatggaag ggatttttgg ctatcaggaa gcgccgagcg
      781 tcagcgaagt cgccgtctgg tttatttatc tcatcccggc gctggtggca tttgctctgc
      841 caccacgcgc aggggcgaca gcgtctcgct ccgcgtaatc ggtcagtttc acctgattta
      901 cgtaaaaacc cgcttcggcg ggtttttgct tttggagggg cagaaagatg aatgactgtc
      961 gactgcaaag ccggcgctcc ggtgaacgct tcacggccaa tcgctgtgca tccggccaca
     1021 gttgacggga atcaccccct gtggacttcc tcaggtcgac attaggttag gctcacctaa
     1081 gttcatcgcc ccctgtggtg ctcaccccca ggagcatcac tagttcacac aggaaagatg
     1141 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1201 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1261 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgcg
     1321 ggcggcagcg aaggcggcgg cagcgaacat catcatcatc atcatggcag cgaacgtaaa
     1381 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     1441 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     1501 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactacc
     1561 ctgacctatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
     1621 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     1681 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     1741 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     1801 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     1861 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     1921 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     1981 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     2041 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata accaggcatc
     2101 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2161 tgaacgctct c
//