BBa_M36709 1 BBa_M36709 Gemini Protein Generator 2011-05-02T11:00:00Z 2015-05-08T01:14:06Z Gemini, a Bifunctional Enzymatic and Fluorescent Reporter of Gene Expression. PLOS One. Lance Martin et al. A medium strength RBS expressing Gemini, ending with a strong terminator. false false _848_ 0 9654 9 Not in stock false Test Actuator false Jeff Quinn and Wyatt Woodson component2118634 1 BBa_E0051 component2118635 1 BBa_B0010 component2118629 1 BBa_B0032 annotation2118635 1 BBa_B0010 range2118635 1 968 1047 annotation2118634 1 BBa_E0051 range2118634 1 14 967 annotation2118629 1 BBa_B0032 range2118629 1 1 13 BBa_M36011 1 BBa_M36011 Fur-regulated (Fe) Promoter 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z 1. Gunter K, Toupet C, Schupp T. Characterization of an iron-regulated promoter involved in desferrioxamine B synthesis in Streptomyces pilosus: repressor-binding site and homology to the diphtheria toxin gene promoter. Journal of bacteriology. 1993;175(11):3295-302. Available at: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=204726&tool=pmcentrez&rendertype=abstract. pPS-PCR is a promoter which is controlled by the iron-repressive transcription factor, Fur. false false _848_ 0 9646 9 Not in stock false Based on deletion analysis of natural S. lividans gene false Brent Townshend annotation2119770 1 SpeI range2119770 1 159 164 annotation2119769 1 start range2119769 1 112 112 annotation2119768 1 -10 range2119768 1 103 108 annotation2119771 1 Possible repressor region range2119771 1 102 120 annotation2119767 1 -35 range2119767 1 82 87 annotation2119772 1 SalI range2119772 1 95 100 BBa_M36731 1 BBa_M36731 EfeU elemental iron (II) transporter + 5' UTR (medium) + terminator + Fur promoter 2011-05-02T11:00:00Z 2015-05-08T01:14:06Z Basic parts. This composite part has our actuator and sensor combined into a single sequence. false false _848_ 0 9545 9 Not in stock false After the terminator of the actual part, we have the Fur promoter. We're planning to place a universal sensor before this part (to trigger expression of EfeU) and a universal actuator after (to trigger expression of a signal when iron is low). false Tom McLaughlin component2118613 1 BBa_M36011 component2118612 1 BBa_M36729 annotation2118612 1 BBa_M36729 range2118612 1 1 960 annotation2118613 1 BBa_M36011 range2118613 1 961 1124 BBa_M36001 1 BBa_M36001 5' Bicistronic UTR (medium), does not include ATG start 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z Vivek Mutalik et al. c/o BIOFAB Emeryville. 5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information. false false _848_ 0 52 432 Not in stock false Please see long description. false Drew Endy BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_M36042 1 BBa_M36042 EfeU elemental iron (II) transporter 2011-04-30T11:00:00Z 2015-05-08T01:14:02Z From wild-type Nissle 1917 E. coli. The sequence came from the Ecogene entry EG13860. This is the reconstructed gene for the EfeU iron transporter. This gene is inactive in K-12 strain E. coli, but has been characterized in the Nissle 1917 strain. (Grobe, Scherer et al. PMID: 16987175.) false false _848_ 0 9545 9 Not in stock false The sequence was conceptually reconstructed from the frame-shifted version. The Ecogene entry has an "N" for the nucleotide which is uncertain, but a comment in that entry says that the correct amino acid is likely glycine. Thus we chose N = G in the final sequence. false Tom McLaughlin annotation2119195 1 REGLE motif range2119195 1 385 399 annotation2119177 1 REGLE motif range2119177 1 28 42 annotation2119094 1 Start codon range2119094 1 1 3 annotation2119197 1 Stop codon range2119197 1 829 831 annotation2119198 1 Repaired frameshift (see notes) range2119198 1 106 106 BBa_M36729 1 BBa_M36729 EfeU elemental iron (II) transporter + 5' UTR (medium) + terminator 2011-05-01T11:00:00Z 2015-05-08T01:14:06Z Basic parts. This composite part has the EfeU iron transporter downstream from a medium 5' Bicistronic UTR. There is a terminator at the end. false false _848_ 0 9545 9 Not in stock false We are hoping that this 5' UTR will cause enough expression of the EfeU protein to measure, without producing so much that the organism dies due to excessive iron uptake or other ion imbalances. false Tom McLaughlin component2118528 1 BBa_M36001 component2118530 1 BBa_M36010 component2118529 1 BBa_M36042 annotation2118528 1 BBa_M36001 range2118528 1 1 47 annotation2118530 1 BBa_M36010 range2118530 1 879 960 annotation2118529 1 BBa_M36042 range2118529 1 48 878 BBa_E0051 1 BBa_E0051 lacZa.GFP fusion 2009-03-01T12:00:00Z 2015-08-31T04:07:25Z amino acids 3-60 from lacZ and amino acids 2-end of GFP (E0043) This part is a fusion protein between GFP and the alpha fragment of lacZ. It can be measured via fluorescence from GFP or through an assay for beta-galactosidase in a strain containing the omega fragment of lacZ. false false _41_ 0 22 84 Not in stock false The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc. false Austin Che annotation2001728 1 GFP range2001728 1 238 954 annotation2001726 1 lacZa range2001726 1 1 180 annotation2001727 1 linker range2001727 1 181 237 BBa_M36732 1 BBa_M36732 EfeU iron (II) transporter + 5' UTR (medium) + terminator + Fur promoter + Gemini 2011-05-02T11:00:00Z 2015-05-08T01:14:06Z Basic parts. This composite part has our actuator and sensor combined into a single sequence, followed by Gemini. false false _848_ 0 9545 9 Not in stock false - false Tom McLaughlin component2118711 1 BBa_M36709 component2118701 1 BBa_M36731 annotation2118701 1 BBa_M36731 range2118701 1 1 1124 annotation2118711 1 BBa_M36709 range2118711 1 1125 2171 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_M36010 1 BBa_M36010 Transcription Terminator (Strong) 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z Guillaume Cambray et al. c/o BIOFAB Emeryville E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator. false true _848_ 0 52 432 Not in stock false please see long description false Drew Endy BBa_M36732_sequence 1 tatagagggtattaataatgtatggattaaagggggaggcataacaaatgtttgttccgtttctcattatgttgcgcgaaggacttgaagccgcgctgattgtcagtttgattgccagctatcttaagcgtacccagcgaggccgatggattggtgtgatgtggattggcgtgttgcttgccgctgcgttgtgcctgggcttgggtatcttcattaacgaaaccaccggcgaatttccgcaaaaagaacaggaactgtttgaaggtatcgtggcggtgatcgccgtggtgatccttacctggatggttttctggatgcgcaaagtgtcgcgcaacgtcaaagtgcaactggaacaggcagtcgatagcgcattgcagcgtggaaatcatcatggctgggcgctggtgatgatggtcttttttgccgttgcaagggaagggctggagtcggtctttttcctgctggcggcatttcaacaagatgtcgggatctggccgccgctgggtgcaatgctcggtcttgctactgccgtggtgctaggcttcctgctctactggggcggtattcgcctcaatcttggtgcattttttaaatggaccagcctgtttattctcttcgtcgccgcagggctggcagctggtgccattcgcgcatttcatgaagccggattgtggaaccactttcaggaaatcgccttcgatatgagtgcggtgctctcaactcactcgctgtttggcacgctgatggaagggatttttggctatcaggaagcgccgagcgtcagcgaagtcgccgtctggtttatttatctcatcccggcgctggtggcatttgctctgccaccacgcgcaggggcgacagcgtctcgctccgcgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtcgactgcaaagccggcgctccggtgaacgcttcacggccaatcgctgtgcatccggccacagttgacgggaatcaccccctgtggacttcctcaggtcgacattaggttaggctcacctaagttcatcgccccctgtggtgctcacccccaggagcatcactagttcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M36001_sequence 1 tatagagggtattaataatgtatggattaaagggggaggcataacaa BBa_M36709_sequence 1 tcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M36011_sequence 1 gactgcaaagccggcgctccggtgaacgcttcacggccaatcgctgtgcatccggccacagttgacgggaatcaccccctgtggacttcctcaggtcgacattaggttaggctcacctaagttcatcgccccctgtggtgctcacccccaggagcatcactagt BBa_E0051_sequence 1 atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_M36729_sequence 1 tatagagggtattaataatgtatggattaaagggggaggcataacaaatgtttgttccgtttctcattatgttgcgcgaaggacttgaagccgcgctgattgtcagtttgattgccagctatcttaagcgtacccagcgaggccgatggattggtgtgatgtggattggcgtgttgcttgccgctgcgttgtgcctgggcttgggtatcttcattaacgaaaccaccggcgaatttccgcaaaaagaacaggaactgtttgaaggtatcgtggcggtgatcgccgtggtgatccttacctggatggttttctggatgcgcaaagtgtcgcgcaacgtcaaagtgcaactggaacaggcagtcgatagcgcattgcagcgtggaaatcatcatggctgggcgctggtgatgatggtcttttttgccgttgcaagggaagggctggagtcggtctttttcctgctggcggcatttcaacaagatgtcgggatctggccgccgctgggtgcaatgctcggtcttgctactgccgtggtgctaggcttcctgctctactggggcggtattcgcctcaatcttggtgcattttttaaatggaccagcctgtttattctcttcgtcgccgcagggctggcagctggtgccattcgcgcatttcatgaagccggattgtggaaccactttcaggaaatcgccttcgatatgagtgcggtgctctcaactcactcgctgtttggcacgctgatggaagggatttttggctatcaggaagcgccgagcgtcagcgaagtcgccgtctggtttatttatctcatcccggcgctggtggcatttgctctgccaccacgcgcaggggcgacagcgtctcgctccgcgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_B0032_sequence 1 tcacacaggaaag BBa_M36010_sequence 1 tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_M36731_sequence 1 tatagagggtattaataatgtatggattaaagggggaggcataacaaatgtttgttccgtttctcattatgttgcgcgaaggacttgaagccgcgctgattgtcagtttgattgccagctatcttaagcgtacccagcgaggccgatggattggtgtgatgtggattggcgtgttgcttgccgctgcgttgtgcctgggcttgggtatcttcattaacgaaaccaccggcgaatttccgcaaaaagaacaggaactgtttgaaggtatcgtggcggtgatcgccgtggtgatccttacctggatggttttctggatgcgcaaagtgtcgcgcaacgtcaaagtgcaactggaacaggcagtcgatagcgcattgcagcgtggaaatcatcatggctgggcgctggtgatgatggtcttttttgccgttgcaagggaagggctggagtcggtctttttcctgctggcggcatttcaacaagatgtcgggatctggccgccgctgggtgcaatgctcggtcttgctactgccgtggtgctaggcttcctgctctactggggcggtattcgcctcaatcttggtgcattttttaaatggaccagcctgtttattctcttcgtcgccgcagggctggcagctggtgccattcgcgcatttcatgaagccggattgtggaaccactttcaggaaatcgccttcgatatgagtgcggtgctctcaactcactcgctgtttggcacgctgatggaagggatttttggctatcaggaagcgccgagcgtcagcgaagtcgccgtctggtttatttatctcatcccggcgctggtggcatttgctctgccaccacgcgcaggggcgacagcgtctcgctccgcgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtcgactgcaaagccggcgctccggtgaacgcttcacggccaatcgctgtgcatccggccacagttgacgggaatcaccccctgtggacttcctcaggtcgacattaggttaggctcacctaagttcatcgccccctgtggtgctcacccccaggagcatcactagt BBa_M36042_sequence 1 atgtttgttccgtttctcattatgttgcgcgaaggacttgaagccgcgctgattgtcagtttgattgccagctatcttaagcgtacccagcgaggccgatggattggtgtgatgtggattggcgtgttgcttgccgctgcgttgtgcctgggcttgggtatcttcattaacgaaaccaccggcgaatttccgcaaaaagaacaggaactgtttgaaggtatcgtggcggtgatcgccgtggtgatccttacctggatggttttctggatgcgcaaagtgtcgcgcaacgtcaaagtgcaactggaacaggcagtcgatagcgcattgcagcgtggaaatcatcatggctgggcgctggtgatgatggtcttttttgccgttgcaagggaagggctggagtcggtctttttcctgctggcggcatttcaacaagatgtcgggatctggccgccgctgggtgcaatgctcggtcttgctactgccgtggtgctaggcttcctgctctactggggcggtattcgcctcaatcttggtgcattttttaaatggaccagcctgtttattctcttcgtcgccgcagggctggcagctggtgccattcgcgcatttcatgaagccggattgtggaaccactttcaggaaatcgccttcgatatgagtgcggtgctctcaactcactcgctgtttggcacgctgatggaagggatttttggctatcaggaagcgccgagcgtcagcgaagtcgccgtctggtttatttatctcatcccggcgctggtggcatttgctctgccaccacgcgcaggggcgacagcgtctcgctccgcgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z