##gff-version 3
##sequence-region BBa_M36732 1 2171
BBa_M36732	.	engineered_region	1	1124	.	+	0	ID=BBa_M36731;Name=BBa_M36731
BBa_M36732	.	engineered_region	1	960	.	+	0	ID=BBa_M36729;Name=BBa_M36729;Parent=BBa_M36731
BBa_M36732	.	terminator	879	960	.	+	0	ID=BBa_M36010;Name=BBa_M36010;Parent=BBa_M36729
BBa_M36732	.	CDS	48	878	.	+	0	ID=BBa_M36042;Name=BBa_M36042;Parent=BBa_M36729
BBa_M36732	.	start_codon	48	50	.	+	0	ID=annotation2119094;Name=Start codon;Parent=BBa_M36042
BBa_M36732	.	stop_codon	876	878	.	+	0	ID=annotation2119197;Name=Stop codon;Parent=BBa_M36042
BBa_M36732	.	tag	75	89	.	+	0	ID=annotation2119177;Name=REGLE motif;Parent=BBa_M36042
BBa_M36732	.	sequence_feature	153	153	.	+	0	ID=annotation2119198;Name=Repaired frameshift (see notes);Parent=BBa_M36042
BBa_M36732	.	tag	432	446	.	+	0	ID=annotation2119195;Name=REGLE motif;Parent=BBa_M36042
BBa_M36732	.	ribosome_entry_site	1	47	.	+	0	ID=BBa_M36001;Name=BBa_M36001;Parent=BBa_M36729
BBa_M36732	.	promoter	961	1124	.	+	0	ID=BBa_M36011;Name=BBa_M36011;Parent=BBa_M36731
BBa_M36732	.	promoter	1063	1068	.	+	0	ID=annotation2119768;Name=-10;Parent=BBa_M36011
BBa_M36732	.	promoter	1042	1047	.	+	0	ID=annotation2119767;Name=-35;Parent=BBa_M36011
BBa_M36732	.	start_codon	1072	1072	.	+	0	ID=annotation2119769;Name=start;Parent=BBa_M36011
BBa_M36732	.	sequence_feature	1119	1124	.	+	0	ID=annotation2119770;Name=SpeI;Parent=BBa_M36011
BBa_M36732	.	non_covalent_binding_site	1062	1080	.	+	0	ID=annotation2119771;Name=Possible repressor region;Parent=BBa_M36011
BBa_M36732	.	sequence_feature	1055	1060	.	+	0	ID=annotation2119772;Name=SalI;Parent=BBa_M36011
BBa_M36732	.	engineered_region	1125	2171	.	+	0	ID=BBa_M36709;Name=BBa_M36709
BBa_M36732	.	ribosome_entry_site	1125	1137	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_M36709
BBa_M36732	.	ribosome_entry_site	1131	1134	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_M36732	.	ribosome_entry_site	1125	1137	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_M36732	.	engineered_region	1125	1137	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_M36732	.	terminator	2092	2171	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M36709
BBa_M36732	.	engineered_region	2092	2171	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M36732	.	stem_loop	2103	2146	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M36732	.	engineered_region	1138	2091	.	+	0	ID=BBa_E0051;Name=BBa_E0051;Parent=BBa_M36709
BBa_M36732	.	CDS	1375	2091	.	+	0	ID=annotation2001728;Name=GFP;Parent=BBa_E0051
BBa_M36732	.	sequence_feature	1318	1374	.	+	0	ID=annotation2001727;Name=linker;Parent=BBa_E0051
BBa_M36732	.	CDS	1138	1317	.	+	0	ID=annotation2001726;Name=lacZa;Parent=BBa_E0051
>BBa_M36732
tatagagggtattaataatgtatggattaaagggggaggcataacaaatgtttgttccgtttctcattatgttgcgcga
aggacttgaagccgcgctgattgtcagtttgattgccagctatcttaagcgtacccagcgaggccgatggattggtgtg
atgtggattggcgtgttgcttgccgctgcgttgtgcctgggcttgggtatcttcattaacgaaaccaccggcgaatttc
cgcaaaaagaacaggaactgtttgaaggtatcgtggcggtgatcgccgtggtgatccttacctggatggttttctggat
gcgcaaagtgtcgcgcaacgtcaaagtgcaactggaacaggcagtcgatagcgcattgcagcgtggaaatcatcatggc
tgggcgctggtgatgatggtcttttttgccgttgcaagggaagggctggagtcggtctttttcctgctggcggcatttc
aacaagatgtcgggatctggccgccgctgggtgcaatgctcggtcttgctactgccgtggtgctaggcttcctgctcta
ctggggcggtattcgcctcaatcttggtgcattttttaaatggaccagcctgtttattctcttcgtcgccgcagggctg
gcagctggtgccattcgcgcatttcatgaagccggattgtggaaccactttcaggaaatcgccttcgatatgagtgcgg
tgctctcaactcactcgctgtttggcacgctgatggaagggatttttggctatcaggaagcgccgagcgtcagcgaagt
cgccgtctggtttatttatctcatcccggcgctggtggcatttgctctgccaccacgcgcaggggcgacagcgtctcgc
tccgcgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaaga
tgaatgactgtcgactgcaaagccggcgctccggtgaacgcttcacggccaatcgctgtgcatccggccacagttgacg
ggaatcaccccctgtggacttcctcaggtcgacattaggttaggctcacctaagttcatcgccccctgtggtgctcacc
cccaggagcatcactagttcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgac
tgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagagg
cccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcag
cgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaa
ttagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccc
ttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatg
ctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaga
actatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaa
tgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagc
gttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgt
ccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgg
gattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctc