BBa_M36732
type: ComponentDefinition
persistent identity: BBa M36732
display id: BBa_M36732
version: 1
was generated by: iGEM to SBOL conversion
description: EfeU iron (II) transporter + 5' UTR (medium) + terminator + Fur promoter + Gemini
created: 2011-05-02T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: Basic parts.
owned by: james
owned by: myers
mutable description: This composite part has our actuator and sensor combined into a single sequence, followed by Gemini.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9545
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Tom McLaughlin
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M36709
component: BBa_M36731
sequence annotation: BBa_M36731
sequence annotation: BBa_M36709
sequence: https://synbiohub.org/public/igem/BBa_M36732_sequence/1

BBa_M36709
type: Component
persistent identity: component2118711
display id: component2118711
version: 1
top level: BBa_M36732
definition: BBa_M36709
access: public

BBa_M36731
type: Component
persistent identity: component2118701
display id: component2118701
version: 1
top level: BBa_M36732
definition: BBa_M36731
access: public

BBa_M36731
type: SequenceAnnotation
persistent identity: annotation2118701
display id: annotation2118701
version: 1
top level: BBa_M36732
location: https://synbiohub.org/public/igem/BBa_M36732/annotation2118701/range2118701/1
component: BBa_M36731

https://synbiohub.org/public/igem/BBa_M36732/annotation2118701/range2118701/1
type: Range
persistent identity: range2118701
display id: range2118701
version: 1
top level: BBa_M36732
direction: direction/reverse
start: 1
end: 1124
orientation: inline

BBa_M36709
type: SequenceAnnotation
persistent identity: annotation2118711
display id: annotation2118711
version: 1
top level: BBa_M36732
location: https://synbiohub.org/public/igem/BBa_M36732/annotation2118711/range2118711/1
component: BBa_M36709

https://synbiohub.org/public/igem/BBa_M36732/annotation2118711/range2118711/1
type: Range
persistent identity: range2118711
display id: range2118711
version: 1
top level: BBa_M36732
direction: direction/reverse
start: 1125
end: 2171
orientation: inline

BBa_M36731
type: ComponentDefinition
persistent identity: BBa M36731
display id: BBa_M36731
version: 1
was generated by: iGEM to SBOL conversion
description: EfeU elemental iron (II) transporter + 5' UTR (medium) + terminator + Fur promoter
created: 2011-05-02T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: Basic parts.
owned by: james
owned by: myers
mutable description: This composite part has our actuator and sensor combined into a single sequence.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9545
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: After the terminator of the actual part, we have the Fur promoter. We're planning to place a universal sensor before this part (to trigger expression of EfeU) and a universal actuator after (to trigger expression of a signal when iron is low).
star: false
creator: Tom McLaughlin
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M36011
component: BBa_M36729
sequence annotation: BBa_M36729
sequence annotation: BBa_M36011
sequence: https://synbiohub.org/public/igem/BBa_M36731_sequence/1

BBa_M36011
type: Component
persistent identity: component2118613
display id: component2118613
version: 1
top level: BBa_M36731
definition: BBa_M36011
access: public

BBa_M36729
type: Component
persistent identity: component2118612
display id: component2118612
version: 1
top level: BBa_M36731
definition: BBa_M36729
access: public

BBa_M36729
type: SequenceAnnotation
persistent identity: annotation2118612
display id: annotation2118612
version: 1
top level: BBa_M36731
location: https://synbiohub.org/public/igem/BBa_M36731/annotation2118612/range2118612/1
component: BBa_M36729

https://synbiohub.org/public/igem/BBa_M36731/annotation2118612/range2118612/1
type: Range
persistent identity: range2118612
display id: range2118612
version: 1
top level: BBa_M36731
direction: direction/reverse
start: 1
end: 960
orientation: inline

BBa_M36011
type: SequenceAnnotation
persistent identity: annotation2118613
display id: annotation2118613
version: 1
top level: BBa_M36731
location: https://synbiohub.org/public/igem/BBa_M36731/annotation2118613/range2118613/1
component: BBa_M36011

https://synbiohub.org/public/igem/BBa_M36731/annotation2118613/range2118613/1
type: Range
persistent identity: range2118613
display id: range2118613
version: 1
top level: BBa_M36731
direction: direction/reverse
start: 961
end: 1124
orientation: inline

BBa_M36709
type: ComponentDefinition
persistent identity: BBa M36709
display id: BBa_M36709
version: 1
was generated by: iGEM to SBOL conversion
description: Gemini Protein Generator
created: 2011-05-02T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: Gemini, a Bifunctional Enzymatic and Fluorescent Reporter of Gene Expression. PLOS One. Lance Martin et al.
owned by: james
owned by: myers
mutable description: A medium strength RBS expressing Gemini, ending with a strong terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9654
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Test Actuator
star: false
creator: Jeff Quinn and Wyatt Woodson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_E0051
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_E0051
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_M36709_sequence/1

BBa_B0010
type: Component
persistent identity: component2118635
display id: component2118635
version: 1
top level: BBa_M36709
definition: BBa_B0010
access: public

BBa_E0051
type: Component
persistent identity: component2118634
display id: component2118634
version: 1
top level: BBa_M36709
definition: BBa_E0051
access: public

BBa_B0032
type: Component
persistent identity: component2118629
display id: component2118629
version: 1
top level: BBa_M36709
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2118629
display id: annotation2118629
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118629/range2118629/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_M36709/annotation2118629/range2118629/1
type: Range
persistent identity: range2118629
display id: range2118629
version: 1
top level: BBa_M36709
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0051
type: SequenceAnnotation
persistent identity: annotation2118634
display id: annotation2118634
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118634/range2118634/1
component: BBa_E0051

https://synbiohub.org/public/igem/BBa_M36709/annotation2118634/range2118634/1
type: Range
persistent identity: range2118634
display id: range2118634
version: 1
top level: BBa_M36709
direction: direction/reverse
start: 14
end: 967
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2118635
display id: annotation2118635
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118635/range2118635/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M36709/annotation2118635/range2118635/1
type: Range
persistent identity: range2118635
display id: range2118635
version: 1
top level: BBa_M36709
direction: direction/forward
start: 968
end: 1047
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_M36011
type: ComponentDefinition
persistent identity: BBa M36011
display id: BBa_M36011
version: 1
was generated by: iGEM to SBOL conversion
description: Fur-regulated (Fe) Promoter
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: 1. Gunter K, Toupet C, Schupp T. Characterization of an iron-regulated promoter involved in desferrioxamine B synthesis in Streptomyces pilosus: repressor-binding site and homology to the diphtheria toxin gene promoter. Journal of bacteriology. 1993;175(11):3295-302. Available at: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=204726&tool=pmcentrez&rendertype=abstract.
owned by: james
owned by: myers
mutable description: pPS-PCR is a promoter which is controlled by the iron-repressive transcription factor, Fur.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9646
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Based on deletion analysis of natural S. lividans gene
star: false
creator: Brent Townshend
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: -35
sequence annotation: SalI
sequence annotation: Possible repressor region
sequence annotation: -10
sequence annotation: start
sequence annotation: SpeI
sequence: https://synbiohub.org/public/igem/BBa_M36011_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2119767
display id: annotation2119767
version: 1
top level: BBa_M36011
location: https://synbiohub.org/public/igem/BBa_M36011/annotation2119767/range2119767/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_M36011/annotation2119767/range2119767/1
type: Range
persistent identity: range2119767
display id: range2119767
version: 1
top level: BBa_M36011
direction: direction/forward
start: 82
end: 87
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2119772
display id: annotation2119772
version: 1
top level: BBa_M36011
location: https://synbiohub.org/public/igem/BBa_M36011/annotation2119772/range2119772/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M36011/annotation2119772/range2119772/1
type: Range
persistent identity: range2119772
display id: range2119772
version: 1
top level: BBa_M36011
direction: direction/forward
start: 95
end: 100
orientation: inline

Possible repressor region
type: SequenceAnnotation
persistent identity: annotation2119771
display id: annotation2119771
version: 1
top level: BBa_M36011
location: https://synbiohub.org/public/igem/BBa_M36011/annotation2119771/range2119771/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36011/annotation2119771/range2119771/1
type: Range
persistent identity: range2119771
display id: range2119771
version: 1
top level: BBa_M36011
direction: direction/forward
start: 102
end: 120
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2119768
display id: annotation2119768
version: 1
top level: BBa_M36011
location: https://synbiohub.org/public/igem/BBa_M36011/annotation2119768/range2119768/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_M36011/annotation2119768/range2119768/1
type: Range
persistent identity: range2119768
display id: range2119768
version: 1
top level: BBa_M36011
direction: direction/forward
start: 103
end: 108
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2119769
display id: annotation2119769
version: 1
top level: BBa_M36011
location: https://synbiohub.org/public/igem/BBa_M36011/annotation2119769/range2119769/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M36011/annotation2119769/range2119769/1
type: Range
persistent identity: range2119769
display id: range2119769
version: 1
top level: BBa_M36011
direction: direction/forward
start: 112
end: 112
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2119770
display id: annotation2119770
version: 1
top level: BBa_M36011
location: https://synbiohub.org/public/igem/BBa_M36011/annotation2119770/range2119770/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M36011/annotation2119770/range2119770/1
type: Range
persistent identity: range2119770
display id: range2119770
version: 1
top level: BBa_M36011
direction: direction/forward
start: 159
end: 164
orientation: inline

BBa_M36729
type: ComponentDefinition
persistent identity: BBa M36729
display id: BBa_M36729
version: 1
was generated by: iGEM to SBOL conversion
description: EfeU elemental iron (II) transporter + 5' UTR (medium) + terminator
created: 2011-05-01T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: Basic parts.
owned by: james
owned by: myers
mutable description: This composite part has the EfeU iron transporter downstream from a medium 5' Bicistronic UTR. There is a terminator at the end.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9545
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We are hoping that this 5' UTR will cause enough expression of the EfeU protein to measure, without producing so much that the organism dies due to excessive iron uptake or other ion imbalances.
star: false
creator: Tom McLaughlin
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M36001
component: BBa_M36010
component: BBa_M36042
sequence annotation: BBa_M36001
sequence annotation: BBa_M36042
sequence annotation: BBa_M36010
sequence: https://synbiohub.org/public/igem/BBa_M36729_sequence/1

BBa_M36001
type: Component
persistent identity: component2118528
display id: component2118528
version: 1
top level: BBa_M36729
definition: BBa_M36001
access: public

BBa_M36010
type: Component
persistent identity: component2118530
display id: component2118530
version: 1
top level: BBa_M36729
definition: BBa_M36010
access: public

BBa_M36042
type: Component
persistent identity: component2118529
display id: component2118529
version: 1
top level: BBa_M36729
definition: BBa_M36042
access: public

BBa_M36001
type: SequenceAnnotation
persistent identity: annotation2118528
display id: annotation2118528
version: 1
top level: BBa_M36729
location: https://synbiohub.org/public/igem/BBa_M36729/annotation2118528/range2118528/1
component: BBa_M36001

https://synbiohub.org/public/igem/BBa_M36729/annotation2118528/range2118528/1
type: Range
persistent identity: range2118528
display id: range2118528
version: 1
top level: BBa_M36729
direction: direction/reverse
start: 1
end: 47
orientation: inline

BBa_M36042
type: SequenceAnnotation
persistent identity: annotation2118529
display id: annotation2118529
version: 1
top level: BBa_M36729
location: https://synbiohub.org/public/igem/BBa_M36729/annotation2118529/range2118529/1
component: BBa_M36042

https://synbiohub.org/public/igem/BBa_M36729/annotation2118529/range2118529/1
type: Range
persistent identity: range2118529
display id: range2118529
version: 1
top level: BBa_M36729
direction: direction/reverse
start: 48
end: 878
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2118530
display id: annotation2118530
version: 1
top level: BBa_M36729
location: https://synbiohub.org/public/igem/BBa_M36729/annotation2118530/range2118530/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36729/annotation2118530/range2118530/1
type: Range
persistent identity: range2118530
display id: range2118530
version: 1
top level: BBa_M36729
direction: direction/reverse
start: 879
end: 960
orientation: inline

BBa_E0051
type: ComponentDefinition
persistent identity: BBa E0051
display id: BBa_E0051
version: 1
was generated by: iGEM to SBOL conversion
description: lacZa.GFP fusion
created: 2009-03-01T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: amino acids 3-60 from lacZ and amino acids 2-end of GFP (E0043)
owned by: james
owned by: myers
mutable description: This part is a fusion protein between GFP and the alpha fragment of lacZ. It can be measured via fluorescence from GFP or through an assay for beta-galactosidase in a strain containing the omega fragment of lacZ.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 22
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc.
star: false
creator: Austin Che
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: lacZa
sequence annotation: linker
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_E0051_sequence/1

lacZa
type: SequenceAnnotation
persistent identity: annotation2001726
display id: annotation2001726
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001726/range2001726/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0051/annotation2001726/range2001726/1
type: Range
persistent identity: range2001726
display id: range2001726
version: 1
top level: BBa_E0051
direction: direction/forward
start: 1
end: 180
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2001727
display id: annotation2001727
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001727/range2001727/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E0051/annotation2001727/range2001727/1
type: Range
persistent identity: range2001727
display id: range2001727
version: 1
top level: BBa_E0051
direction: direction/forward
start: 181
end: 237
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2001728
display id: annotation2001728
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001728/range2001728/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0051/annotation2001728/range2001728/1
type: Range
persistent identity: range2001728
display id: range2001728
version: 1
top level: BBa_E0051
direction: direction/forward
start: 238
end: 954
orientation: inline

BBa_M36001
type: ComponentDefinition
persistent identity: BBa M36001
display id: BBa_M36001
version: 1
was generated by: iGEM to SBOL conversion
description: 5' Bicistronic UTR (medium), does not include ATG start
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Vivek Mutalik et al. c/o BIOFAB Emeryville.
owned by: james
owned by: myers
mutable description: 5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 52
owning group id: 432
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Please see long description.
star: false
creator: Drew Endy
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M36001_sequence/1

BBa_M36010
type: ComponentDefinition
persistent identity: BBa M36010
display id: BBa_M36010
version: 1
was generated by: iGEM to SBOL conversion
description: Transcription Terminator (Strong)
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Guillaume Cambray et al. c/o BIOFAB Emeryville
owned by: james
owned by: myers
mutable description: E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
discontinued: false
dominant: true
group u list: _848_
m user id: 0
owner id: 52
owning group id: 432
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: please see long description
star: false
creator: Drew Endy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_M36010_sequence/1

BBa_M36042
type: ComponentDefinition
persistent identity: BBa M36042
display id: BBa_M36042
version: 1
was generated by: iGEM to SBOL conversion
description: EfeU elemental iron (II) transporter
created: 2011-04-30T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: From wild-type Nissle 1917 E. coli. The sequence came from the Ecogene entry EG13860.
owned by: james
owned by: myers
mutable description: This is the reconstructed gene for the EfeU iron transporter. This gene is inactive in K-12 strain E. coli, but has been characterized in the Nissle 1917 strain. (Grobe, Scherer et al. PMID: 16987175.)
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9545
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The sequence was conceptually reconstructed from the frame-shifted version. The Ecogene entry has an "N" for the nucleotide which is uncertain, but a comment in that entry says that the correct amino acid is likely glycine. Thus we chose N = G in the final sequence.
star: false
creator: Tom McLaughlin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: REGLE motif
sequence annotation: Repaired frameshift (see notes)
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_M36042_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2119094
display id: annotation2119094
version: 1
top level: BBa_M36042
location: https://synbiohub.org/public/igem/BBa_M36042/annotation2119094/range2119094/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M36042/annotation2119094/range2119094/1
type: Range
persistent identity: range2119094
display id: range2119094
version: 1
top level: BBa_M36042
direction: direction/forward
start: 1
end: 3
orientation: inline

REGLE motif
type: SequenceAnnotation
persistent identity: annotation2119177
display id: annotation2119177
version: 1
top level: BBa_M36042
location: https://synbiohub.org/public/igem/BBa_M36042/annotation2119177/range2119177/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_M36042/annotation2119177/range2119177/1
type: Range
persistent identity: range2119177
display id: range2119177
version: 1
top level: BBa_M36042
direction: direction/forward
start: 28
end: 42
orientation: inline

Repaired frameshift (see notes)
type: SequenceAnnotation
persistent identity: annotation2119198
display id: annotation2119198
version: 1
top level: BBa_M36042
location: https://synbiohub.org/public/igem/BBa_M36042/annotation2119198/range2119198/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M36042/annotation2119198/range2119198/1
type: Range
persistent identity: range2119198
display id: range2119198
version: 1
top level: BBa_M36042
direction: direction/forward
start: 106
end: 106
orientation: inline

REGLE motif
type: SequenceAnnotation
persistent identity: annotation2119195
display id: annotation2119195
version: 1
top level: BBa_M36042
location: https://synbiohub.org/public/igem/BBa_M36042/annotation2119195/range2119195/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_M36042/annotation2119195/range2119195/1
type: Range
persistent identity: range2119195
display id: range2119195
version: 1
top level: BBa_M36042
direction: direction/forward
start: 385
end: 399
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2119197
display id: annotation2119197
version: 1
top level: BBa_M36042
location: https://synbiohub.org/public/igem/BBa_M36042/annotation2119197/range2119197/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M36042/annotation2119197/range2119197/1
type: Range
persistent identity: range2119197
display id: range2119197
version: 1
top level: BBa_M36042
direction: direction/forward
start: 829
end: 831
orientation: inline

https://synbiohub.org/public/igem/BBa_M36732_sequence/1
type: Sequence
persistent identity: BBa M36732 sequence
display id: BBa_M36732_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatagagggtattaataatgtatggattaaagggggaggcataacaaatgtttgttccgtttctcattatgttgcgcgaaggacttgaagccgcgctgattgtcagtttgattgccagctatcttaagcgtacccagcgaggccgatggattggtgtgatgtggattggcgtgttgcttgccgctgcgttgtgcctgggcttgggtatcttcattaacgaaaccaccggcgaatttccgcaaaaagaacaggaactgtttgaaggtatcgtggcggtgatcgccgtggtgatccttacctggatggttttctggatgcgcaaagtgtcgcgcaacgtcaaagtgcaactggaacaggcagtcgatagcgcattgcagcgtggaaatcatcatggctgggcgctggtgatgatggtcttttttgccgttgcaagggaagggctggagtcggtctttttcctgctggcggcatttcaacaagatgtcgggatctggccgccgctgggtgcaatgctcggtcttgctactgccgtggtgctaggcttcctgctctactggggcggtattcgcctcaatcttggtgcattttttaaatggaccagcctgtttattctcttcgtcgccgcagggctggcagctggtgccattcgcgcatttcatgaagccggattgtggaaccactttcaggaaatcgccttcgatatgagtgcggtgctctcaactcactcgctgtttggcacgctgatggaagggatttttggctatcaggaagcgccgagcgtcagcgaagtcgccgtctggtttatttatctcatcccggcgctggtggcatttgctctgccaccacgcgcaggggcgacagcgtctcgctccgcgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtcgactgcaaagccggcgctccggtgaacgcttcacggccaatcgctgtgcatccggccacagttgacgggaatcaccccctgtggacttcctcaggtcgacattaggttaggctcacctaagttcatcgccccctgtggtgctcacccccaggagcatcactagttcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36731_sequence/1
type: Sequence
persistent identity: BBa M36731 sequence
display id: BBa_M36731_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatagagggtattaataatgtatggattaaagggggaggcataacaaatgtttgttccgtttctcattatgttgcgcgaaggacttgaagccgcgctgattgtcagtttgattgccagctatcttaagcgtacccagcgaggccgatggattggtgtgatgtggattggcgtgttgcttgccgctgcgttgtgcctgggcttgggtatcttcattaacgaaaccaccggcgaatttccgcaaaaagaacaggaactgtttgaaggtatcgtggcggtgatcgccgtggtgatccttacctggatggttttctggatgcgcaaagtgtcgcgcaacgtcaaagtgcaactggaacaggcagtcgatagcgcattgcagcgtggaaatcatcatggctgggcgctggtgatgatggtcttttttgccgttgcaagggaagggctggagtcggtctttttcctgctggcggcatttcaacaagatgtcgggatctggccgccgctgggtgcaatgctcggtcttgctactgccgtggtgctaggcttcctgctctactggggcggtattcgcctcaatcttggtgcattttttaaatggaccagcctgtttattctcttcgtcgccgcagggctggcagctggtgccattcgcgcatttcatgaagccggattgtggaaccactttcaggaaatcgccttcgatatgagtgcggtgctctcaactcactcgctgtttggcacgctgatggaagggatttttggctatcaggaagcgccgagcgtcagcgaagtcgccgtctggtttatttatctcatcccggcgctggtggcatttgctctgccaccacgcgcaggggcgacagcgtctcgctccgcgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtcgactgcaaagccggcgctccggtgaacgcttcacggccaatcgctgtgcatccggccacagttgacgggaatcaccccctgtggacttcctcaggtcgacattaggttaggctcacctaagttcatcgccccctgtggtgctcacccccaggagcatcactagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36709_sequence/1
type: Sequence
persistent identity: BBa M36709 sequence
display id: BBa_M36709_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36011_sequence/1
type: Sequence
persistent identity: BBa M36011 sequence
display id: BBa_M36011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gactgcaaagccggcgctccggtgaacgcttcacggccaatcgctgtgcatccggccacagttgacgggaatcaccccctgtggacttcctcaggtcgacattaggttaggctcacctaagttcatcgccccctgtggtgctcacccccaggagcatcactagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36729_sequence/1
type: Sequence
persistent identity: BBa M36729 sequence
display id: BBa_M36729_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatagagggtattaataatgtatggattaaagggggaggcataacaaatgtttgttccgtttctcattatgttgcgcgaaggacttgaagccgcgctgattgtcagtttgattgccagctatcttaagcgtacccagcgaggccgatggattggtgtgatgtggattggcgtgttgcttgccgctgcgttgtgcctgggcttgggtatcttcattaacgaaaccaccggcgaatttccgcaaaaagaacaggaactgtttgaaggtatcgtggcggtgatcgccgtggtgatccttacctggatggttttctggatgcgcaaagtgtcgcgcaacgtcaaagtgcaactggaacaggcagtcgatagcgcattgcagcgtggaaatcatcatggctgggcgctggtgatgatggtcttttttgccgttgcaagggaagggctggagtcggtctttttcctgctggcggcatttcaacaagatgtcgggatctggccgccgctgggtgcaatgctcggtcttgctactgccgtggtgctaggcttcctgctctactggggcggtattcgcctcaatcttggtgcattttttaaatggaccagcctgtttattctcttcgtcgccgcagggctggcagctggtgccattcgcgcatttcatgaagccggattgtggaaccactttcaggaaatcgccttcgatatgagtgcggtgctctcaactcactcgctgtttggcacgctgatggaagggatttttggctatcaggaagcgccgagcgtcagcgaagtcgccgtctggtttatttatctcatcccggcgctggtggcatttgctctgccaccacgcgcaggggcgacagcgtctcgctccgcgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0051_sequence/1
type: Sequence
persistent identity: BBa E0051 sequence
display id: BBa_E0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36001_sequence/1
type: Sequence
persistent identity: BBa M36001 sequence
display id: BBa_M36001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatagagggtattaataatgtatggattaaagggggaggcataacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36010_sequence/1
type: Sequence
persistent identity: BBa M36010 sequence
display id: BBa_M36010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36042_sequence/1
type: Sequence
persistent identity: BBa M36042 sequence
display id: BBa_M36042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgttccgtttctcattatgttgcgcgaaggacttgaagccgcgctgattgtcagtttgattgccagctatcttaagcgtacccagcgaggccgatggattggtgtgatgtggattggcgtgttgcttgccgctgcgttgtgcctgggcttgggtatcttcattaacgaaaccaccggcgaatttccgcaaaaagaacaggaactgtttgaaggtatcgtggcggtgatcgccgtggtgatccttacctggatggttttctggatgcgcaaagtgtcgcgcaacgtcaaagtgcaactggaacaggcagtcgatagcgcattgcagcgtggaaatcatcatggctgggcgctggtgatgatggtcttttttgccgttgcaagggaagggctggagtcggtctttttcctgctggcggcatttcaacaagatgtcgggatctggccgccgctgggtgcaatgctcggtcttgctactgccgtggtgctaggcttcctgctctactggggcggtattcgcctcaatcttggtgcattttttaaatggaccagcctgtttattctcttcgtcgccgcagggctggcagctggtgccattcgcgcatttcatgaagccggattgtggaaccactttcaggaaatcgccttcgatatgagtgcggtgctctcaactcactcgctgtttggcacgctgatggaagggatttttggctatcaggaagcgccgagcgtcagcgaagtcgccgtctggtttatttatctcatcccggcgctggtggcatttgctctgccaccacgcgcaggggcgacagcgtctcgctccgcgtaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z