LOCUS       BBa_M36733              1211 bp    DNA     linear       14-Nov-2024
DEFINITION  Fur promoter + Gemini
ACCESSION   BBa_M36733
VERSION     BBa_M36733.1  
FEATURES             Location/Qualifiers
     promoter        82..87
                     /label=-35
     promoter        103..108
                     /label=-10
     misc_feature    112..112
                     /label=start
     misc_feature    159..164
                     /label=SpeI
     misc_feature    102..120
                     /label=Possible repressor region
     misc_feature    95..100
                     /label=SalI
     promoter        1..164
                     /label=BBa_M36011
     RBS             165..177
                     /label=RBS-3\Weak
     RBS             171..174
                     /label=RBS
     misc_feature    165..177
                     /label=BBa_B0032
     RBS             165..177
                     /label=BBa_B0032
     CDS             178..357
                     /label=lacZa
     misc_feature    358..414
                     /label=linker
     CDS             415..1131
                     /label=GFP
     misc_feature    178..1131
                     /label=BBa_E0051
     stem_loop       1143..1186
                     /label=stem_loop
     misc_feature    1132..1211
                     /label=BBa_B0010
     terminator      1132..1211
                     /label=BBa_B0010
     misc_feature    165..1211
                     /label=BBa_M36709
ORIGIN
        1 gactgcaaag ccggcgctcc ggtgaacgct tcacggccaa tcgctgtgca tccggccaca
       61 gttgacggga atcaccccct gtggacttcc tcaggtcgac attaggttag gctcacctaa
      121 gttcatcgcc ccctgtggtg ctcaccccca ggagcatcac tagttcacac aggaaagatg
      181 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      241 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      301 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgcg
      361 ggcggcagcg aaggcggcgg cagcgaacat catcatcatc atcatggcag cgaacgtaaa
      421 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      481 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      541 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactacc
      601 ctgacctatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      661 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      721 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      781 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      841 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
      901 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
      961 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     1021 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     1081 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata accaggcatc
     1141 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1201 tgaacgctct c
//