##gff-version 3
##sequence-region BBa_M36748 1 1041
BBa_M36748	.	promoter	1	131	.	+	0	ID=BBa_K118011;Name=BBa_K118011
BBa_M36748	.	non_covalent_binding_site	1	42	.	+	0	ID=annotation1972435;Name=cAMP receptor protein binding site;Parent=BBa_K118011
BBa_M36748	.	non_covalent_binding_site	101	131	.	+	0	ID=annotation1972436;Name=RNA polymerase binding site;Parent=BBa_K118011
BBa_M36748	.	engineered_region	140	1041	.	+	0	ID=BBa_K173005;Name=BBa_K173005
BBa_M36748	.	engineered_region	140	979	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K173005
BBa_M36748	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_M36748	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M36748	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M36748	.	ribosome_entry_site	140	151	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_M36748	.	conserved_region	144	147	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M36748	.	CDS	158	817	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_M36748	.	tag	778	811	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_M36748	.	engineered_tag	818	842	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_M36748	.	CDS	161	777	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_M36748	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_M36748	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M36748	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M36748	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M36748	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M36748	.	promoter	988	1041	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K173005
BBa_M36748	.	non_covalent_binding_site	988	1006	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_M36748	.	promoter	1030	1035	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_M36748	.	promoter	1007	1012	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_M36748	.	non_covalent_binding_site	1013	1031	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_M36748	.	engineered_region	988	1041	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
>BBa_M36748
actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgtt
gttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggacatactagagaaagaggagaaatactaga
tgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaac
ccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgac
gccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttac
gtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctac
agaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatat
gcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggg
aaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagc
cttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgac
gaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgata
gagatactgagcac