BBa_M36759 1 BBa_M36759 Channelrhodopsin-2 actuator 2012-12-04T12:00:00Z 2015-05-08T01:14:06Z Stanford Optogenetics Center: http://www.stanford.edu/group/dlab/optogenetics/sequence_info.html Channelrhodopsin-2 (ChR2) is a light-gated calcium ion channel that is part of the microbial opsin protein family found in the green alga Chlamydomonas reinhardtii. It is a light-gated calcium ion channel localized to the membrane. Designed for HeLa cells. false false _848_ 0 15012 9 Not in stock true There were no design considerations that we had to deal with. false Beatriz Collazo, Paul Hichwa, John Pluvinage component2213450 1 BBa_M36760 component2213453 1 BBa_K678019 annotation2213453 1 BBa_K678019 range2213453 1 976 1208 annotation2213450 1 BBa_M36760 range2213450 1 1 967 BBa_K678019 1 BGH pA BGH poly A, mammalian terminator 2011-09-03T11:00:00Z 2015-05-08T01:13:04Z From plasmid pcDNA3.1 from Invitrogen BGH poly A, terminator for use in mammalian cells false false _882_ 0 8341 9 It's complicated false Compatible with the Plug'n'Play assembly standard false DTU-Denmark-2 annotation2127352 1 Linker 4 range2127352 1 1 8 annotation2127353 1 BGH range2127353 1 9 233 BBa_M36760 1 BBa_M36760 Channelrhodopsin-2 light-gated Ca2+ channel 2012-12-04T12:00:00Z 2015-05-08T01:14:06Z Optimized for expression and sequenced by the Deisseroth Lab at Stanford University. We obtained the ChR2 coding sequence from the Stanford Optogenetics Resource Center. http://www.stanford.edu/group/dlab/optogenetics/sequence_info.html This part encodes the microbial opsin protein, channelrhodopsin-2. It is a light-gated calcium ion membrane channel. false false _848_ 0 15012 9 Not in stock false We added a e5'UTR-bG from Gene Designer from DNA 2.0, kozak consensus sequence including the start codon (gccaccatg) sequence and a stop codon (tag). No mutations were needed for repeats in the coding sequence. false Beatriz Collazo, Paul Hichwa, John Pluvinage BBa_K678019_sequence 1 attccgatctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_M36760_sequence 1 gaatacaagctacttgttctttttgcacgccaccatggactatggcggcgctttgtctgccgtcggacgcgaacttttgttcgttactaatcctgtggtggtgaacgggtccgtcctggtccctgaggatcaatgttactgtgccggatggattgaatctcgcggcacgaacggcgctcagaccgcgtcaaatgtcctgcagtggcttgcagcaggattcagcattttgctgctgatgttctatgcctaccaaacctggaaatctacatgcggctgggaggagatctatgtgtgcgccattgaaatggttaaggtgattctcgagttcttttttgagtttaagaatccctctatgctctaccttgccacaggacaccgggtgcagtggctgcgctatgcagagtggctgctcacttgtcctgtcatccttatccacctgagcaacctcaccggcctgagcaacgactacagcaggagaaccatgggactccttgtctcagacatcgggactatcgtgtggggggctaccagcgccatggcaaccggctatgttaaagtcatcttcttttgtcttggattgtgctatggcgcgaacacattttttcacgccgccaaagcatatatcgagggttatcatactgtgccaaagggtcggtgccgccaggtcgtgaccggcatggcatggctgtttttcgtgagctggggtatgttcccaattctcttcattttggggcccgaaggttttggcgtcctgagcgtctatggctccaccgtaggtcacacgattattgatctgatgagtaaaaattgttgggggttgttgggacactacctgcgcgtcctgatccacgagcacatattgattcacggagatatccgcaaaaccaccaaactgaacatcggcggaacggagatcgaggtcgagactctcgtcgaagacgaagccgaggccggagccgtgccatag BBa_M36759_sequence 1 gaatacaagctacttgttctttttgcacgccaccatggactatggcggcgctttgtctgccgtcggacgcgaacttttgttcgttactaatcctgtggtggtgaacgggtccgtcctggtccctgaggatcaatgttactgtgccggatggattgaatctcgcggcacgaacggcgctcagaccgcgtcaaatgtcctgcagtggcttgcagcaggattcagcattttgctgctgatgttctatgcctaccaaacctggaaatctacatgcggctgggaggagatctatgtgtgcgccattgaaatggttaaggtgattctcgagttcttttttgagtttaagaatccctctatgctctaccttgccacaggacaccgggtgcagtggctgcgctatgcagagtggctgctcacttgtcctgtcatccttatccacctgagcaacctcaccggcctgagcaacgactacagcaggagaaccatgggactccttgtctcagacatcgggactatcgtgtggggggctaccagcgccatggcaaccggctatgttaaagtcatcttcttttgtcttggattgtgctatggcgcgaacacattttttcacgccgccaaagcatatatcgagggttatcatactgtgccaaagggtcggtgccgccaggtcgtgaccggcatggcatggctgtttttcgtgagctggggtatgttcccaattctcttcattttggggcccgaaggttttggcgtcctgagcgtctatggctccaccgtaggtcacacgattattgatctgatgagtaaaaattgttgggggttgttgggacactacctgcgcgtcctgatccacgagcacatattgattcacggagatatccgcaaaaccaccaaactgaacatcggcggaacggagatcgaggtcgagactctcgtcgaagacgaagccgaggccggagccgtgccatagtactagagattccgatctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z