BBa_M36761 1 MDA Melamine deaminase coding sequence 2013-06-09T11:00:00Z 2015-05-08T01:14:06Z The original gene coding for the protein of interest was isolated from a species of Pseudomonas (http://www.ncbi.nlm.nih.gov/nuccore/11890744). This part is the coding sequence for the enzyme melamine deaminase, optimized for usage in E. coli. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations. false false _848_ 0 17944 9 Not in stock false The TriA gene???s codons were modified from the original and optimized for E. coli to a Codon Adaptation Index (CAI) of .76. After that, some palindromic and mRNA destabilizing sequences were removed to increase the stability of our DNA during synthesis. false Ray Paul Laureano-Mercado & Mark Kirollos annotation2329380 1 Melamine Deaminase range2329380 1 1 1422 annotation2329382 1 Stop (TAG) range2329382 1 1423 1425 annotation2329381 1 Start (ATG) range2329381 1 1 3 BBa_M36761_sequence 1 atgcaaactctgtctattcagcatggtaccctggtaacaatggatcagtaccgtcgcgttctgggcgacagctgggtgcatgtacaggatggtcgtattgttgctctgggtgtgcacgcagaaagcgttccaccgccggcggatcgtgtaatcgacgctcgtggtaaagttgtactgccgggcttcattaacgctcatactcacgttaaccagatcctgctgcgcggtggtccgtcccatgggcgccagttatatgattggctgttcaatgttctgtatccaggtcagaaagcaatgcgtccggaagatgttgcggtggcagtgcgtctttattgtgctgaggccgttcgctccggcatcaccacgattaacgataacgcggactccgcaatctatccgggtaacatcgaagcggcaatggcggtgtacggggaagtgggtgtacgtgttgtttacgcgcgcatgtttttcgatcgcatggacggccgcatccaaggctatgttgacgcgctgaaagcacgttcaccgcaggtggagctgtgctctatcatggaggaaacggccgtcgcgaaagatcgtatcactgcgctgtctgaccagtatcacggtaccgcgggcggtcgtatcagcgtgtggccagccccggctatcaccccggcggttaccgttgaaggtatgcgttgggcacaggcattcgcgcgcgaccgcgccgtaatgtggaccctgcacatggcggagagcgatcatgatgaacgtctgcactggatgtcgccggctgaatacatggagtgttacggtttactggacgagcgcctgcaggtcgcccattgtgtttacttcgatcgcaaggacgttcgcctgctgcatcgccacaacgtaaaggttgcttctcaggttgttagcaatgcgtatctgggttccggcgttgcgccggttccggaaatggtagaacgtggtatggccgttggtattggtaccgatgatggcaactgcaacgattccgtgaacatgattggcgatatgaagttcatggctcacattcatcgtgcagtacaccgcgacgcagacgttctgaccccggaaaagatcctggaaatggccaccatcgacggtgcgcgcagtctgggcatggaccatgaaattggcagcatcgaaaccggcaaacgtgctgacctgattctgctggatctgcgtcacccgcaaactaccccgcaccaccacctggcagctaccatcgtgtttcaggcatacggtaacgaagttgacaccgttttgatcgacggtaacgtcgtgatggaaaaccgccgtctgtcatttctgccgccggaacgtgaactggcttttctggaagaagctcagtcccgtgccactgcgatcctgcagcgtgcaaacatggttgcgaacccggcttggcgtagcctgtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z