BBa_M36779 1 BBa_M36779 RpoS gene sequence 2013-10-25T11:00:00Z 2015-05-08T01:14:06Z It naturally exists in E Coli. This is the gene sequence that encode the protein for sigma factor, which gives positive feedback to the promoter in our project design and magnifies the performance of it. false false _848_ 0 19064 9 Not in stock false It might trigger the transcription controlled by other promoters that are regulated by the same sigma factor. false Sylvia Yang BBa_M36783 1 BBa_M36783 GadA promoter 2013-10-25T11:00:00Z 2015-05-08T01:14:06Z It naturally exists in E Coli. Use of it in past project: http://parts.igem.org/wiki/index.php?title=Part:BBa_K318512#. This is a pH responsive promoter that initiates transcription as pH drops. We use it to activate our transcription here. false false _848_ 0 19064 9 Not in stock false It may not be pH sensitive enough for ideal result. false Sylvia Yang BBa_M36781 1 BBa_M36781 BCD13 2013-10-25T11:00:00Z 2015-05-08T01:14:06Z From the paper "Precise and reliable gene expression via standard transcription and translation initiation elements." It is a BSD site that has slightly below average activity. false false _848_ 0 19064 9 In stock false The BCD might be too weak to activate the translation. false Sylvia Yang BBa_M36776 1 BBa_M36776 pH sensor 2013-12-03T12:00:00Z 2015-05-08T01:14:06Z The promoter, GadA sequence comes from 2010 Wisconsin iGem team, the BCD13 sequence from paper "Precise and reliable gene expression via standard transcription and translation initiation elements", the Rpos sequence from "Factor RpoD - Escherichia Coli (strain K12)". This is a cell with the designed pH sensor that would produce more GFP signals as the pH drops. false false _848_ 0 19064 9 Not in stock false A) the sigma factor may change the normal cell behavior. B) low pH would kill cells. false Sylvia Yang component2371578 1 BBa_M36783 component2371579 1 BBa_M36781 component2371580 1 BBa_M36779 annotation2371579 1 BBa_M36781 range2371579 1 255 346 annotation2371580 1 BBa_M36779 range2371580 1 347 1063 annotation2371578 1 BBa_M36783 range2371578 1 1 254 BBa_M36783_sequence 1 ctttcctgcacagtctgaacggggtgtacacgccgtatatgcaggataaacaataattaatttgatcgcccgaacagcaatgtttgggcgatttttattacgataataaagtctgtttttaatattatcatgttaaatgtttatattataaaaagtcgtttttctgcttaggattttgttatttaaattaagcctgtaatgccttgcttccattgcggataaatcctacttttttattgccttcaaataaattt BBa_M36776_sequence 1 ctttcctgcacagtctgaacggggtgtacacgccgtatatgcaggataaacaataattaatttgatcgcccgaacagcaatgtttgggcgatttttattacgataataaagtctgtttttaatattatcatgttaaatgtttatattataaaaagtcgtttttctgcttaggattttgttatttaaattaagcctgtaatgccttgcttccattgcggataaatcctacttttttattgccttcaaataaattttactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcaatggaggctttctaatgagtcagaatacgctgaaagttcatgatttaaatgaagatgcggaatttgatgagaacggagttgaggtttttgacgaaaaggccttagtagaacaggaacccagtgataacgatttggccgaagaggaactgttatcgcagggagccacacagcgtgtgttggacgcgactcagctttaccttggtgagattggttattcaccactgttaacggccgaagaagaagtttattttgcgcgtcgcgcactgcgtggagatgtcgcctctcgccgccggatgatcgagagtaacttgcgtctggtggtaaaaattgcccgccgttatggcaatcgtggtctggcgttgctggaccttatcgaagagggcaacctggggctgatccgcgcggtagagaagtttgacccggaacgtggtttccgcttctcaacatacgcaacctggtggattcgccagacgattgaacgggcgattatgaaccaaacccgtactattcgtttgccgattcacatcgtaaaggagctgaacgtttacctgcgaaccgcacgtgagttgtcccataagctggaccatgaaccaagtgcggaagagatcgcagagcaactggataagccagttgatgacgtcagccgtatgcttcgtcttaacgagcgcattacctcggtagacaccccgctgggtggtgattccgaaaaagcgttgctggacatcctggccgat BBa_M36779_sequence 1 agtcagaatacgctgaaagttcatgatttaaatgaagatgcggaatttgatgagaacggagttgaggtttttgacgaaaaggccttagtagaacaggaacccagtgataacgatttggccgaagaggaactgttatcgcagggagccacacagcgtgtgttggacgcgactcagctttaccttggtgagattggttattcaccactgttaacggccgaagaagaagtttattttgcgcgtcgcgcactgcgtggagatgtcgcctctcgccgccggatgatcgagagtaacttgcgtctggtggtaaaaattgcccgccgttatggcaatcgtggtctggcgttgctggaccttatcgaagagggcaacctggggctgatccgcgcggtagagaagtttgacccggaacgtggtttccgcttctcaacatacgcaacctggtggattcgccagacgattgaacgggcgattatgaaccaaacccgtactattcgtttgccgattcacatcgtaaaggagctgaacgtttacctgcgaaccgcacgtgagttgtcccataagctggaccatgaaccaagtgcggaagagatcgcagagcaactggataagccagttgatgacgtcagccgtatgcttcgtcttaacgagcgcattacctcggtagacaccccgctgggtggtgattccgaaaaagcgttgctggacatcctggccgat BBa_M36781_sequence 1 tactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcaatggaggctttctaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z