BBa_M36779
1
BBa_M36779
RpoS gene sequence
2013-10-25T11:00:00Z
2015-05-08T01:14:06Z
It naturally exists in E Coli.
This is the gene sequence that encode the protein for sigma factor, which gives positive feedback to the promoter in our project design and magnifies the performance of it.
false
false
_848_
0
19064
9
Not in stock
false
It might trigger the transcription controlled by other promoters that are regulated by the same sigma factor.
false
Sylvia Yang
BBa_M36783
1
BBa_M36783
GadA promoter
2013-10-25T11:00:00Z
2015-05-08T01:14:06Z
It naturally exists in E Coli.
Use of it in past project: http://parts.igem.org/wiki/index.php?title=Part:BBa_K318512#.
This is a pH responsive promoter that initiates transcription as pH drops. We use it to activate our transcription here.
false
false
_848_
0
19064
9
Not in stock
false
It may not be pH sensitive enough for ideal result.
false
Sylvia Yang
BBa_M36781
1
BBa_M36781
BCD13
2013-10-25T11:00:00Z
2015-05-08T01:14:06Z
From the paper "Precise and reliable gene expression via standard transcription and translation initiation elements."
It is a BSD site that has slightly below average activity.
false
false
_848_
0
19064
9
In stock
false
The BCD might be too weak to activate the translation.
false
Sylvia Yang
BBa_M36776
1
BBa_M36776
pH sensor
2013-12-03T12:00:00Z
2015-05-08T01:14:06Z
The promoter, GadA sequence comes from 2010 Wisconsin iGem team, the BCD13 sequence from paper "Precise and reliable gene expression via standard transcription and translation initiation elements", the Rpos sequence from "Factor RpoD - Escherichia Coli (strain K12)".
This is a cell with the designed pH sensor that would produce more GFP signals as the pH drops.
false
false
_848_
0
19064
9
Not in stock
false
A) the sigma factor may change the normal cell behavior.
B) low pH would kill cells.
false
Sylvia Yang
component2371578
1
BBa_M36783
component2371579
1
BBa_M36781
component2371580
1
BBa_M36779
annotation2371579
1
BBa_M36781
range2371579
1
255
346
annotation2371580
1
BBa_M36779
range2371580
1
347
1063
annotation2371578
1
BBa_M36783
range2371578
1
1
254
BBa_M36783_sequence
1
ctttcctgcacagtctgaacggggtgtacacgccgtatatgcaggataaacaataattaatttgatcgcccgaacagcaatgtttgggcgatttttattacgataataaagtctgtttttaatattatcatgttaaatgtttatattataaaaagtcgtttttctgcttaggattttgttatttaaattaagcctgtaatgccttgcttccattgcggataaatcctacttttttattgccttcaaataaattt
BBa_M36776_sequence
1
ctttcctgcacagtctgaacggggtgtacacgccgtatatgcaggataaacaataattaatttgatcgcccgaacagcaatgtttgggcgatttttattacgataataaagtctgtttttaatattatcatgttaaatgtttatattataaaaagtcgtttttctgcttaggattttgttatttaaattaagcctgtaatgccttgcttccattgcggataaatcctacttttttattgccttcaaataaattttactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcaatggaggctttctaatgagtcagaatacgctgaaagttcatgatttaaatgaagatgcggaatttgatgagaacggagttgaggtttttgacgaaaaggccttagtagaacaggaacccagtgataacgatttggccgaagaggaactgttatcgcagggagccacacagcgtgtgttggacgcgactcagctttaccttggtgagattggttattcaccactgttaacggccgaagaagaagtttattttgcgcgtcgcgcactgcgtggagatgtcgcctctcgccgccggatgatcgagagtaacttgcgtctggtggtaaaaattgcccgccgttatggcaatcgtggtctggcgttgctggaccttatcgaagagggcaacctggggctgatccgcgcggtagagaagtttgacccggaacgtggtttccgcttctcaacatacgcaacctggtggattcgccagacgattgaacgggcgattatgaaccaaacccgtactattcgtttgccgattcacatcgtaaaggagctgaacgtttacctgcgaaccgcacgtgagttgtcccataagctggaccatgaaccaagtgcggaagagatcgcagagcaactggataagccagttgatgacgtcagccgtatgcttcgtcttaacgagcgcattacctcggtagacaccccgctgggtggtgattccgaaaaagcgttgctggacatcctggccgat
BBa_M36779_sequence
1
agtcagaatacgctgaaagttcatgatttaaatgaagatgcggaatttgatgagaacggagttgaggtttttgacgaaaaggccttagtagaacaggaacccagtgataacgatttggccgaagaggaactgttatcgcagggagccacacagcgtgtgttggacgcgactcagctttaccttggtgagattggttattcaccactgttaacggccgaagaagaagtttattttgcgcgtcgcgcactgcgtggagatgtcgcctctcgccgccggatgatcgagagtaacttgcgtctggtggtaaaaattgcccgccgttatggcaatcgtggtctggcgttgctggaccttatcgaagagggcaacctggggctgatccgcgcggtagagaagtttgacccggaacgtggtttccgcttctcaacatacgcaacctggtggattcgccagacgattgaacgggcgattatgaaccaaacccgtactattcgtttgccgattcacatcgtaaaggagctgaacgtttacctgcgaaccgcacgtgagttgtcccataagctggaccatgaaccaagtgcggaagagatcgcagagcaactggataagccagttgatgacgtcagccgtatgcttcgtcttaacgagcgcattacctcggtagacaccccgctgggtggtgattccgaaaaagcgttgctggacatcctggccgat
BBa_M36781_sequence
1
tactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcaatggaggctttctaatg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z