LOCUS       BBa_M36778              1915 bp    DNA     linear       22-May-2024
DEFINITION  DNA damage sensor with Gemini Reporter (weak LexA Generator)
ACCESSION   BBa_M36778
VERSION     BBa_M36778.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             36..82
                     /label=BBa_M36000
     CDS             83..691
                     /label=lexA
     misc_feature    83..83
                     /label=start
     misc_feature    691..691
                     /label=stop
     CDS             83..691
                     /label=BBa_M36050
     terminator      692..773
                     /label=BBa_M36010
     misc_feature    838..841
                     /label=lexA binding
     misc_feature    842..849
                     /label=8 nt spacer
     misc_feature    850..853
                     /label=lexA binding
     promoter        774..868
                     /label=BBa_M36051
     misc_feature    1..868
                     /label=BBa_M36777
     RBS             869..881
                     /label=RBS-3\Weak
     RBS             875..878
                     /label=RBS
     misc_feature    869..881
                     /label=BBa_B0032
     RBS             869..881
                     /label=BBa_B0032
     CDS             882..1061
                     /label=lacZa
     misc_feature    1062..1118
                     /label=linker
     CDS             1119..1835
                     /label=GFP
     misc_feature    882..1835
                     /label=BBa_E0051
     stem_loop       1847..1890
                     /label=stem_loop
     misc_feature    1836..1915
                     /label=BBa_B0010
     terminator      1836..1915
                     /label=BBa_B0010
     misc_feature    869..1915
                     /label=BBa_M36709
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctatag agggtattaa taatgtatgg
       61 attaaagccg gaaacataac aaatgaaagc gttaacggcc aggcaacaag aggtgtttga
      121 tctcatccgt gatcacatca gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca
      181 gcgtttgggg ttccgttccc caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa
      241 aggcgttatt gaaattgttt ccggcgcatc acgcgggatt cgtctgttgc aggaagagga
      301 agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa ccacttctgg cgcaacagca
      361 tattgaaggt cattatcagg tcgatccttc cttattcaag ccgaatgctg atttcctgct
      421 gcgcgtcagc gggatgtcga tgaaagatat cggcattatg gatggtgact tgctggcagt
      481 gcataaaact caggatgtac gtaacggtca ggtcgttgtc gcacgtattg atgacgaagt
      541 taccgttaag cgcctgaaaa aacagggcaa taaagtcgaa ctgttgccag aaaatagcga
      601 gtttaaacca attgtcgttg accttcgtca gcagagcttc accattgaag ggctggcggt
      661 tggggttatt cgcaacggcg actggctgta atcggtcagt ttcacctgat ttacgtaaaa
      721 acccgcttcg gcgggttttt gcttttggag gggcagaaag atgaatgact gtccccttcc
      781 agaattcgat aaatctctgg tttattgtgc agtttatggt tccaaaatcg ccttttgctg
      841 tatatactca cagcataact gtatatactc acacaggaaa gatgaccatg attacggatt
      901 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      961 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1021 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgcgggcggc agcgaaggcg
     1081 gcggcagcga acatcatcat catcatcatg gcagcgaacg taaaggagaa gaacttttca
     1141 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     1201 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     1261 ctactggaaa actacctgtt ccatggccaa cacttgtcac taccctgacc tatggtgttc
     1321 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     1381 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     1441 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     1501 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     1561 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     1621 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     1681 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     1741 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     1801 ttacacatgg catggatgaa ctatacaaat aataaccagg catcaaataa aacgaaaggc
     1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctc
//