BBa_M11082
1
BBa_M11082
ZntR:Zn(II)-responsive regulator of zntA (also MerR transcriptional regulator)
2009-04-17T11:00:00Z
2015-05-08T01:13:53Z
JGI
merR, coding sequence for mercury detecting transcriptional regulator
false
false
_302_
0
4568
302
Not in stock
true
To start the following mercury binding gene
false
Xianyu Meng
annotation2002486
1
start
range2002486
1
1
3
BBa_M36824
1
BBa_M36824
Zinc Sensor
2014-10-21T11:00:00Z
2015-05-08T01:14:06Z
The genomic sequence comes from an E. Coli protein (ZntR) found in basic parts within the registry.
This composite part contains a promoter and ribosome-binding-site, a gene for ZntR, a terminator, and a promoter for ZntA. In the absence of Zinc, the ZntR protein binds to the ZntA binding site. In the presence of zinc, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of ZntA. Essentially, this part acts as a sensor for the presence of zinc.
true
false
_848_
0
24110
9
Discontinued
false
We had to enter silent mutations to change the DNA sequence without affecting the amino acid sequence because there were palindromes greater than 10 bp in every basic part.
false
Leah Chase
component2428886
1
BBa_B0010
component2428890
1
BBa_K190016
component2428885
1
BBa_M11082
component2428883
1
BBa_K190022
annotation2428883
1
BBa_K190022
range2428883
1
1
91
annotation2428885
1
BBa_M11082
range2428885
1
98
511
annotation2428886
1
BBa_B0010
range2428886
1
520
599
annotation2428890
1
BBa_K190016
range2428890
1
608
676
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K190022
1
BBa_K190022
Zinc Promoter (ZntR regulated) with own RBS
2009-08-24T11:00:00Z
2015-05-08T01:11:15Z
Strain: E.coli k.12
The pZntR from E.coli K.12 has a specific RBS site behind it in the genome. Here the RBS site is attachted to the promoter region. The RBS site might influence the activity of the promoter and will be tested in the same way as BBa_K190016.
false
false
_306_
0
4144
9
It's complicated
false
The short sequence did not contain any unwanted restriction sites.
false
Michael Verhoeven
annotation2017703
1
RBS site
range2017703
1
70
91
annotation2017704
1
-35 and -10 region
range2017704
1
34
69
BBa_K190016
1
BBa_K190016
Zinc Promoter (ZntR regulated)
2009-07-14T11:00:00Z
2015-05-08T01:11:15Z
E.coli TOP10
Promoter sequence with recognition site for ZntR transcriptional regulator protein.
false
false
_306_
0
4144
9
It's complicated
false
None
false
Michael Verhoeven
annotation2011504
1
Palindromic binding site for ZntR
range2011504
1
32
53
annotation2011503
1
-35 and -10 region
range2011503
1
35
69
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_M36824_sequence
1
cgtccgctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttaatgagaaaaaacttaaccggaggatactagatgtatcgcatcggccaaattgcaaaattagcgaatgttacaacagatactatccgtttttatgaaaaacaaggattgatggaacatgatagacggacagaaggtggctatcgtctgtatacagaacaagatttgcaaagattacgctttattcgttatgccaaagagttaggctttactctggatgctattactgagttactttcaattagagtcgatcctgcgcatcatacttgtgtagaatcaaaacatattgttgatgctaggctagcagaagttgaggctcgtctaaaagaaatggaaaagatgcgtgattctttaaaaatgttaagcaatgcttgctgtggaaccgctcatgaaagtacatattgttcaattctagaaatacttgaatctggagcgacaaagcaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagcgtccgctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttaat
BBa_M11082_sequence
1
atgtatcgcatcggccaaattgcaaaattagcgaatgttacaacagatactatccgtttttatgaaaaacaaggattgatggaacatgatagacggacagaaggtggctatcgtctgtatacagaacaagatttgcaaagattacgctttattcgttatgccaaagagttaggctttactctggatgctattactgagttactttcaattagagtcgatcctgcgcatcatacttgtgtagaatcaaaacatattgttgatgctaggctagcagaagttgaggctcgtctaaaagaaatggaaaagatgcgtgattctttaaaaatgttaagcaatgcttgctgtggaaccgctcatgaaagtacatattgttcaattctagaaatacttgaatctggagcgacaaagcaataa
BBa_K190016_sequence
1
cgtccgctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttaat
BBa_K190022_sequence
1
cgtccgctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttaatgagaaaaaacttaaccggagga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z