BBa_M36825 1 BBa_M36825 Phenylalanine Hydroxylase 2014-10-22T11:00:00Z 2015-05-08T01:14:06Z Phenylalanine hydroxylase comes from the the PAH gene, and the CDS for this specific part came from the human PAH gene. This part codes for the phenylalanine hydroxylase protein. Phenylalanine hydroxylase is an enzyme that converts phenylalanine substrate into tyrosine. false false _848_ 0 24161 9 Not in stock false Design considerations included not having BsaI restriction sites, palindromic sequences, and whether the toxicity of the produced protein to E. coli (it is not). false Christine Chang, Sonia Targ BBa_M36771 1 BBa_M36771 Strong RBS from DNA 2.0 Gene Designer 2014-10-22T11:00:00Z 2015-05-08T01:14:06Z DNA 2.0 Gene Designer Strong Ribosome Binding Site from DNA 2.0 Gene Designer. false false _848_ 0 24189 9 Not in stock false None. false Preston Lim BBa_M36826 1 BBa_M36826 Phenylalanine Hydroxylase w/ RBS and Terminator 2014-10-23T11:00:00Z 2015-05-08T01:14:06Z RBS: produced by DNA 2.0. It is their standard strong RBS from their pD864. (Sequence unknown) Phenylalanine Hydroxylase: human PAH gene. Terminator: Transcription terminator pT-T7 from bacteriophage This part codes for the phenylalanine hydroxylase protein. Phenylalanine hydroxylase is an enzyme that converts phenylalanine substrate into tyrosine. This specific part includes a strong RBS and a strong terminator. false false _848_ 0 24161 9 Not in stock false We wanted a strong RBS and a strong terminator, as we want increased expression of our protein, which is not toxic. false Christine Chang, Sonia Targ component2429295 1 BBa_M36771 component2429296 1 BBa_M36825 component2429297 1 BBa_M36305 annotation2429296 1 BBa_M36825 range2429296 1 20 1378 annotation2429297 1 BBa_M36305 range2429297 1 1387 1434 annotation2429295 1 BBa_M36771 range2429295 1 1 13 BBa_M36305 1 BBa_M36305 Transcription terminator pT-T7 from bacteriophage 2011-12-08T12:00:00Z 2015-05-08T01:14:04Z T7 Bacteriophage Released HQ 2013 A transcription terminator derived from T7 bacteriophage. Use where transcription should be stopped for the gene of interest upstream of this terminator. false false _848_ 0 11095 9 In stock false None. false Darren Jindal, Raman Nelakanti, Dennis Te BBa_M36825_sequence 1 atgtcgacggctgtgttagaaaatccgggtttgggtcgtaaactgagcgattttggtcaagaaaccagctacatcgaagataactgtaaccagaatggcgcgattagcctgattttcagcctgaaagaggaagtgggcgccttggcgaaagttttgcgcctgtttgaagagaatgacgttaatctgacccatatcgagtctcgccctagccgtctgaaaaaagatgagtacgagttcttcacccatctggacaagcgtagcctgccggcactgacgaacattatcaagattctgcgtcacgacattggtgcaactgttcatgaactgagccgtgacaagaaaaaagacaccgttccgtggttcccgcgtactatccaagagctggaccgcttcgcgaaccagatcctgagctatggtgcagagctggacgcggaccacccgggctttaaagacccggtatatcgtgcgcgtcgtaagcaatttgcggatattgcgtacaattaccgtcacggtcagccgattccgcgtgtcgagtatatggaagaggaaaagaaaacctggggcacggttttcaagaccttgaaaagcctgtacaagactcatgcgtgttatgagtacaaccacattttccctctgctggaaaagtactgcggttttcatgaggataacatcccgcaactggaagatgtgagccagtttctgcagacctgtaccggtttccgtctgcgtccggtggccggtctgttgagctcccgtgactttctgggtggcctggctttccgcgttttccactgtacccagtacattcgccacggtagcaaaccgatgtacaccccggagccagatatctgccatgaactgctgggtcacgtcccgctgttcagcgatcgctccttcgcgcagttctcgcaagaaattggtctggcgagcctgggcgcaccggacgagtatattgaaaaactggcgaccatctactggtttacggtcgagttcggcctgtgcaaacagggcgacagcatcaaggcatacggcgccggcctgctgtctagctttggcgagctgcaatattgcctgagcgaaaagccgaagctgctgccacttgagctggagaaaaccgccattcagaactacacggtgacggaattccaacctctgtattatgttgcagagagctttaatgatgctaaagaaaaagtgcgtaatttcgcggcgacgatcccgcgtccgtttagcgtccgctacgacccgtacacccagcgcattgaagtgctcgacaacacccaacagttgaagatcctggcggattccatcaatagcgagatcggtattttgtgctctgcactgcaaaagatcaagtaa BBa_M36305_sequence 1 ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_M36771_sequence 1 aggaggtaaaaaa BBa_M36826_sequence 1 aggaggtaaaaaatactagatgtcgacggctgtgttagaaaatccgggtttgggtcgtaaactgagcgattttggtcaagaaaccagctacatcgaagataactgtaaccagaatggcgcgattagcctgattttcagcctgaaagaggaagtgggcgccttggcgaaagttttgcgcctgtttgaagagaatgacgttaatctgacccatatcgagtctcgccctagccgtctgaaaaaagatgagtacgagttcttcacccatctggacaagcgtagcctgccggcactgacgaacattatcaagattctgcgtcacgacattggtgcaactgttcatgaactgagccgtgacaagaaaaaagacaccgttccgtggttcccgcgtactatccaagagctggaccgcttcgcgaaccagatcctgagctatggtgcagagctggacgcggaccacccgggctttaaagacccggtatatcgtgcgcgtcgtaagcaatttgcggatattgcgtacaattaccgtcacggtcagccgattccgcgtgtcgagtatatggaagaggaaaagaaaacctggggcacggttttcaagaccttgaaaagcctgtacaagactcatgcgtgttatgagtacaaccacattttccctctgctggaaaagtactgcggttttcatgaggataacatcccgcaactggaagatgtgagccagtttctgcagacctgtaccggtttccgtctgcgtccggtggccggtctgttgagctcccgtgactttctgggtggcctggctttccgcgttttccactgtacccagtacattcgccacggtagcaaaccgatgtacaccccggagccagatatctgccatgaactgctgggtcacgtcccgctgttcagcgatcgctccttcgcgcagttctcgcaagaaattggtctggcgagcctgggcgcaccggacgagtatattgaaaaactggcgaccatctactggtttacggtcgagttcggcctgtgcaaacagggcgacagcatcaaggcatacggcgccggcctgctgtctagctttggcgagctgcaatattgcctgagcgaaaagccgaagctgctgccacttgagctggagaaaaccgccattcagaactacacggtgacggaattccaacctctgtattatgttgcagagagctttaatgatgctaaagaaaaagtgcgtaatttcgcggcgacgatcccgcgtccgtttagcgtccgctacgacccgtacacccagcgcattgaagtgctcgacaacacccaacagttgaagatcctggcggattccatcaatagcgagatcggtattttgtgctctgcactgcaaaagatcaagtaatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z