BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_M36855 1 BBa_M36855 Recombinase-based heat-inducible sensor 2014-10-22T11:00:00Z 2015-05-08T01:14:06Z Source here This part is a recombinase-based heat-inducible sensor false false _848_ 0 24162 9 Not in stock false Size false Anna Jaffe component2429079 1 BBa_M36430 component2429074 1 BBa_B0014 component2429066 1 BBa_K338001 component2429078 1 BBa_J23102 component2429067 1 BBa_M36428 component2429077 1 BBa_I20290 annotation2429079 1 BBa_M36430 range2429079 1 1794 1847 annotation2429067 1 BBa_M36428 range2429067 1 99 1613 annotation2429077 1 BBa_I20290 range2429077 1 1709 1758 annotation2429066 1 BBa_K338001 range2429066 1 1 98 annotation2429074 1 BBa_B0014 range2429074 1 1614 1708 annotation2429078 1 BBa_J23102 range2429078 1 1759 1793 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939303 1 BBa_B0012 component939311 1 BBa_B0011 annotation939303 1 BBa_B0012 range939303 1 1 41 annotation939311 1 BBa_B0011 range939311 1 50 95 BBa_J23102 1 BBa_J23102 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z isolated from library of promoters Released HQ 2013 replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_M36430 1 BBa_M36430 Bxb1 attP - reverse 2014-10-22T11:00:00Z 2015-05-08T01:14:04Z Source Description false false _848_ 0 24162 9 Not in stock false Design false Anna Jaffe BBa_M36428 1 BBa_M36428 RBS, spacer, Bxb1 integrase 2014-10-22T11:00:00Z 2015-05-08T01:14:04Z Source Description false false _848_ 0 24162 9 Not in stock false Design false Anna Jaffe BBa_K338001 1 HSP Heat Shock Promoter (HSP) 2010-10-23T11:00:00Z 2015-05-08T01:12:07Z Modified BBa_K112400 This is a modified K112400 heat shock promoter in BBa standard. false false _454_ 0 6189 9 It's complicated true None false 2010 Caltech iGEM Team BBa_I20290 1 BBa_I20290 Bxb1 attB-Forward 2009-07-10T11:00:00Z 2015-08-31T04:07:40Z upcoming Bxb1 integrase catalyse recombination of Bxb1-attB with Bxb1-AttP. If the 2 sites are in the same molecule, recombination outcome can be:a) excision of the sequence in between ( att sites in parallel orientation) b) sequence inversion (att sites in antiparallel orientation). false false _11_ 0 3868 11 Not in stock false upcoming false Jerome Bonnet annotation2011217 1 core range2011217 1 22 29 annotation2011218 1 GT central dinucleotide range2011218 1 25 26 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_I20290_sequence 1 tcggccggcttgtcgacgacggcggtctccgtcgtcaggatcatccgggc BBa_J23102_sequence 1 ttgacagctagctcagtcctaggtactgtgctagc BBa_M36855_sequence 1 ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaagttagcgagatgaatgcgaggaggatcgatatgcgcgcactggtggtgattcgtttgagccgtgtgaccgatgcaacgacttctcctgaacgtcaactggagtcctgccagcagctgtgcgcgcagcgtggttgggacgttgtgggcgtcgcagaggatttggacgtgagcggtgcagtggacccgttcgaccgcaaacgtcgtccgaatctggcgcgttggctggcgtttgaggaacaaccgtttgacgtgattgttgcgtatcgcgtggaccgtctgacccgttccattcgtcacttgcaacagctggtgcactgggcagaggaccataagaaactggttgtcagcgcgaccgaagcccacttcgatacgaccacgccgtttgctgcggtcgtgattgcgctgatgggtacggttgctcagatggaactggaagctatcaaagaacgtaatcgtagcgccgcgcactttaatatccgtgcgggcaagtaccgcggtagcctgccgccatggggctatttgcctacgcgtgtggatggtgagtggcgtctggttccggacccggttcagcgtgaacgcattctggaagtctaccaccgtgttgtcgataaccatgagccgctgcatttggtcgcgcatgatctgaaccgtcgtggcgtgctgtctccgaaagactacttcgcgcagctgcaaggtcgtgagccgcagggccgtgagtggtccgcgaccgcattgaagcgtagcatgattagcgaagcgatgctgggctatgccacgttgaacggtaagactgttcgtgacgatgacggtgcaccgctggttcgtgctgaaccgatcctgacccgcgagcaactggaagcgctgcgtgcggaactggttaagacgagccgcgcaaagcctgccgtgtccactccgagcctgctgctgcgtgtcctgttctgtgctgtgtgcggcgaaccggcatataagttcgcgggtggtggtcgcaaacatccgcgctaccgctgtcgcagcatgggctttccaaaacactgtggtaacggtaccgtcgctatggccgaatgggacgcgttttgtgaggaacaggtcctggatctgctgggtgatgcggagcgtctggaaaaagtgtgggttgccggtagcgacagcgcggtcgagttagccgaagttaacgccgagctggttgacctgaccagcctgatcggtagcccggcataccgcgcaggcagcccgcaacgcgaggcccttgatgcccgcatcgcggccctggcagcccgccaagaagaactggaaggcttggaggcgcgtccgagcggctgggagtggcgtgaaaccggccaacgcttcggtgattggtggcgcgagcaagacaccgcagccaaaaacacctggctccgttctatgaatgttcgtctgaccttcgacgtgcgtggcggcctgacgcgcacgatcgattttggtgatctgcaggaatacgagcagcatttgcgtctgggttcggtcgtcgagcgcctgcacaccggtatgtcctgatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttcggccggcttgtcgacgacggcggtctccgtcgtcaggatcatccgggcttgacagctagctcagtcctaggtactgtgctagcgggtttgtaccgtacaccactgagaccgcggtggttgaccagacaaaccacgat BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K338001_sequence 1 ccgaggtccttgttgcgaagattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaagttagcgagatgaatgcg BBa_M36430_sequence 1 gggtttgtaccgtacaccactgagaccgcggtggttgaccagacaaaccacgat BBa_M36428_sequence 1 aggaggatcgatatgcgcgcactggtggtgattcgtttgagccgtgtgaccgatgcaacgacttctcctgaacgtcaactggagtcctgccagcagctgtgcgcgcagcgtggttgggacgttgtgggcgtcgcagaggatttggacgtgagcggtgcagtggacccgttcgaccgcaaacgtcgtccgaatctggcgcgttggctggcgtttgaggaacaaccgtttgacgtgattgttgcgtatcgcgtggaccgtctgacccgttccattcgtcacttgcaacagctggtgcactgggcagaggaccataagaaactggttgtcagcgcgaccgaagcccacttcgatacgaccacgccgtttgctgcggtcgtgattgcgctgatgggtacggttgctcagatggaactggaagctatcaaagaacgtaatcgtagcgccgcgcactttaatatccgtgcgggcaagtaccgcggtagcctgccgccatggggctatttgcctacgcgtgtggatggtgagtggcgtctggttccggacccggttcagcgtgaacgcattctggaagtctaccaccgtgttgtcgataaccatgagccgctgcatttggtcgcgcatgatctgaaccgtcgtggcgtgctgtctccgaaagactacttcgcgcagctgcaaggtcgtgagccgcagggccgtgagtggtccgcgaccgcattgaagcgtagcatgattagcgaagcgatgctgggctatgccacgttgaacggtaagactgttcgtgacgatgacggtgcaccgctggttcgtgctgaaccgatcctgacccgcgagcaactggaagcgctgcgtgcggaactggttaagacgagccgcgcaaagcctgccgtgtccactccgagcctgctgctgcgtgtcctgttctgtgctgtgtgcggcgaaccggcatataagttcgcgggtggtggtcgcaaacatccgcgctaccgctgtcgcagcatgggctttccaaaacactgtggtaacggtaccgtcgctatggccgaatgggacgcgttttgtgaggaacaggtcctggatctgctgggtgatgcggagcgtctggaaaaagtgtgggttgccggtagcgacagcgcggtcgagttagccgaagttaacgccgagctggttgacctgaccagcctgatcggtagcccggcataccgcgcaggcagcccgcaacgcgaggcccttgatgcccgcatcgcggccctggcagcccgccaagaagaactggaaggcttggaggcgcgtccgagcggctgggagtggcgtgaaaccggccaacgcttcggtgattggtggcgcgagcaagacaccgcagccaaaaacacctggctccgttctatgaatgttcgtctgaccttcgacgtgcgtggcggcctgacgcgcacgatcgattttggtgatctgcaggaatacgagcagcatttgcgtctgggttcggtcgtcgagcgcctgcacaccggtatgtcctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z