BBa_M36798 1 BBa_M36798 Stress-regulated transcription factor 2014-10-22T11:00:00Z 2015-05-08T01:14:06Z Arabidopsis genome www.ncbi.nlm.nih.gov/nuccore/NC_003075.7?report=genbank&from=13018214&to=13019121&strand=true DREB1A is a transcription factor, naturally found in Arabidopsis, which in interacts with a cis-acting promoter element (operator) induces expression of stress tolerance genes downstream, particularly those related to drought, salt loading and freezing environmental stresses. This provides enhanced tolerance to stress conditions without affecting plant growth. In the context of the project at hand, we are introducing this part in HeLa cells with long-term goals of constructing superior salt-sensing and tolerance mechanisms in mammalian cells. false false _848_ 0 24153 9 Not in stock false N/A false Negin Behzadian BBa_M36900 1 BBa_M36900 Salt Stress Sensor (Eukaryotes) 2014-10-23T11:00:00Z 2015-05-08T01:14:06Z Arabidopsis This part is an NaCl sensor, mainly combining stress-inducible transcription factors from plants with eukaryotic constitutive promoters and terminators, in order to construct an effective salt-sensing mechanism in HeLa (mammalian) cells, that could later be placed upstream of particular naturally encoded endocytosis genes in HeLa cells, to iduce their expression as a way of increasing salt tolerance in mammalian cells. false false _848_ 0 24153 9 Not in stock true couple the DREB1A gene with a friendly PoPS promoter than would not foster retardation of plants/cell death while building stress tolerance false Negin Behzadian component2429174 1 BBa_I712004 component2429175 1 BBa_M36798 component2429178 1 BBa_K678012 component2429179 1 BBa_M36799 annotation2429178 1 BBa_K678012 range2429178 1 1320 1458 annotation2429175 1 BBa_M36798 range2429175 1 661 1311 annotation2429174 1 BBa_I712004 range2429174 1 1 654 annotation2429179 1 BBa_M36799 range2429179 1 1467 2301 BBa_M36799 1 BBa_M36799 stress-inducible promoter (rd29A) 2014-10-23T11:00:00Z 2015-05-08T01:14:06Z Arabidopsis This is the promoter for the DREB1A gene. DREB1A is a transcription factor the production of which induces the hyperexpression of genes involved in environmental stress tolerance (thus far in plants) and thus improves drought/salt tolerance. The rd29A promoter has been found to couple effectively with DREB1A with minimal growth hindrance or retardation in the engineered plants. false false _848_ 0 24153 9 Not in stock false N/A false Negin Behzadian BBa_I712004 1 BBa_I712004 CMV promoter 2007-10-18T11:00:00Z 2015-08-31T04:07:45Z pcDNA3 by Invitrogen a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included. false false _130_ 0 1896 9 It's complicated false Cloned into pSB1AC3 vector. false Andrej Ondracka BBa_K678012 1 BBa_K678012 SV40 poly A, terminator for mammalian cells 2011-09-03T11:00:00Z 2015-05-08T01:13:04Z From plasmid pcDNA3.1 from Invitrogen SV40 poly A, terminator for use in mammalian cells false false _882_ 0 8341 9 It's complicated false Compatible with the Plug'n'Play assembly standard false DTU-Denmark-2 annotation2127354 1 Linker 4 range2127354 1 1 8 annotation2127355 1 SV40 range2127355 1 9 139 BBa_M36900_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagatgaactcattttctgctttttctgaaatgtttggctccgattacgagtcttcggtttcctcaggcggtgattatattccgacgcttgcgagcagctgccccaagaaaccggcgggtcgtaagaagtttcgtgagactcgtcacccaatatacagaggagttcgtcggagaaactccggtaagtgggtttgtgaggttagagaaccaaacaagaaaacaaggatttggctcggaacatttcaaaccgctgagatggcagctcgagctcacgacgttgccgctttagcccttcgtggccgatcagcctgtctcaatttcgctgactcggcttggagactccgaatcccggaatcaacttgcgctaaggacatccaaaaggcggcggctgaagctgcgttggcgtttcaggatgagatgtgtgatgcgacgacggatcatggcttcgacatggaggagacgttggtggaggctatttacacggcggaacagagcgaaaatgcgttttatatgcacgatgaggcgatgtttgagatgccgagtttgttggctaatatggcagaagggatgcttttgccgcttccgtccgtacagtggaatcataatcatgaagtcgacggcgatgatgacgacgtatcgttatggagttattaatactagagattccgataacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtactagagaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattattatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggtaaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttccatttgactagggtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatcttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttcttgacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaaggagaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacgtaaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacattttaggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaataaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacacgcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtccctttatctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaactagaaaacaatcatcaggaataaagggtttgattacttctattggaaagaaaaaaatctttggaaaatgga BBa_I712004_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaat BBa_M36799_sequence 1 aggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattattatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggtaaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttccatttgactagggtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatcttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttcttgacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaaggagaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacgtaaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacattttaggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaataaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacacgcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtccctttatctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaactagaaaacaatcatcaggaataaagggtttgattacttctattggaaagaaaaaaatctttggaaaatgga BBa_K678012_sequence 1 attccgataacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctg BBa_M36798_sequence 1 atgaactcattttctgctttttctgaaatgtttggctccgattacgagtcttcggtttcctcaggcggtgattatattccgacgcttgcgagcagctgccccaagaaaccggcgggtcgtaagaagtttcgtgagactcgtcacccaatatacagaggagttcgtcggagaaactccggtaagtgggtttgtgaggttagagaaccaaacaagaaaacaaggatttggctcggaacatttcaaaccgctgagatggcagctcgagctcacgacgttgccgctttagcccttcgtggccgatcagcctgtctcaatttcgctgactcggcttggagactccgaatcccggaatcaacttgcgctaaggacatccaaaaggcggcggctgaagctgcgttggcgtttcaggatgagatgtgtgatgcgacgacggatcatggcttcgacatggaggagacgttggtggaggctatttacacggcggaacagagcgaaaatgcgttttatatgcacgatgaggcgatgtttgagatgccgagtttgttggctaatatggcagaagggatgcttttgccgcttccgtccgtacagtggaatcataatcatgaagtcgacggcgatgatgacgacgtatcgttatggagttattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z