BBa_M36915 1 BBa_M36915 Codes for the benzylalcohol acetyltransferase (BEAT) enzyme 2012-12-06T12:00:00Z 2015-05-08T01:14:07Z The cDNA and amino acid sequences were developed by Natalia Dudareva et. al Dudareva, Natalia, John C. D'Auria, Kyoung Hee Nam, Robert A. Raguso, and Eran Pichersky. "Acetyl-CoA:benzylalcohol Acetyltransferase - an Enzyme Involved in Floral Scent Production in Clarkia Breweri." The Plant Journal 14.3 (1998): 297-304. NOTE: this sequence does not contain a start codon; choose an RBS/translation unit that ends with a start codon, or add one to the beginning of this sequence. The benzylalcohol acetyltransferase (BEAT) enzyme, the sequence of which is contained is this part, was extracted from the petals of the clarkia breweri flower. The BEAT enzyme catalyzes the production of benzyl acetate (a volatile, odorous compound) from benzyl alcohol false true _848_ 0 15040 9 Not in stock false The DNA sequence was extracted from a flower but optimized for a host E. Coli bacteria (e.g. deleted repeated sequences, optimized GC content). false Rohit Talreja, Kelly Nguyen, Jacqueline Tao BBa_M36913 1 BBa_M36913 takes PoPs input 2012-12-06T12:00:00Z 2015-05-08T01:14:06Z BIOFAB standard parts library Mutalik, Vivek, et al. ???Rules for Precision Gene Expression via Reusable Standard Biological Parts???. This part is a bicistronically designed (BCD) translational unit. It is used to ensure reliable translation of genes of interest in a construct false false _848_ 0 15040 9 Not in stock false This sequence has a start codon at the end of the coding sequence false Rohit Talreja, Kelly Nguyen, Jacqueline Tao annotation2213664 1 Start Codon range2213664 1 86 88 BBa_M36912 1 BBa_M36912 Transcription terminator, works well with BBa_M36913 RBS 2012-12-06T12:00:00Z 2015-05-08T01:14:06Z BIOFAB standards parts library Mutalik, Vivek, et al. ???Rules for Precision Gene Expression via Reusable Standard Biological Parts??? Released HQ 2013 This part is a strong transcription terminator false false _848_ 0 15040 9 In stock false Works well with translational RBS BBa_M36913 to effectively terminate transcription false Rohit Talreja, Kelly Nguyen, Jacqueline Tao annotation2213668 1 Stop Codon range2213668 1 13 15 annotation2213669 1 Stop Codon range2213669 1 70 72 BBa_M36916 1 BBa_M36916 This series of parts codes for the expression of the benzylalcohol acetyltransferase enzyme 2012-12-06T12:00:00Z 2015-05-08T01:14:07Z The terminator and bicistronic RBS come from the BIOFAB standards part database Mutalik, Vivek, et al. ???Rules for Precision Gene Expression via Reusable Standard Biological Parts??? The coding DNA sequence was extracted by Natalia Dudareva et al Dudareva, Natalia, John C. D'Auria, Kyoung Hee Nam, Robert A. Raguso, and Eran Pichersky. "Acetyl-CoA:benzylalcohol Acetyltransferase - an Enzyme Involved in Floral Scent Production in Clarkia Breweri." The Plant Journal 14.3 (1998): 297-304. This is a coding sequence consisting of the: BBa_M36913: bicistronic RBS BBa_M36915: coding DNA sequence for the benzylalcohol acetyltransferase (BEAT) enzyme. This enzyme converts benzyl alcohol into benzylacetate (a volatile, odorous compound excreted from the petals of many species of flowers). BBa_M36912: transcription terminator false false _848_ 0 15040 9 Not in stock false Needed to optimize part for several strengths of PoPS input and expression in E. coli false Rohit Talreja, Kelly Nguyen, Jacqueline Tao component2213672 1 BBa_M36912 component2213675 1 BBa_M36915 component2213674 1 BBa_M36913 annotation2213674 1 BBa_M36913 range2213674 1 138 225 annotation2213672 1 BBa_M36912 range2213672 1 1 129 annotation2213675 1 BBa_M36915 range2213675 1 234 1529 BBa_M36916_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgggggagcgtttctgatgtactagagaacgtgaccatgcatagcaaaaaactgctgaaaccgagcattccgaccccgaaccatctgcagaaactgaacctgagcctgctggatcagattcagattccgttttatgtgggcctgatttttcattatgaaaccctgagcgataacagcgatattaccctgagcaaactggaaagcagcctgagcgaaaccctgaccctgtattatcatgtggcgggccgctataacggcaccgattgcgtgattgaatgcaacgatcagggcattggctatgtggaaaccgcgtttgatgtggaactgcatcagtttctgctgggcgaagaaagcaacaacctggatctgctggtgggcctgagcggctttctgagcgaaaccgaaaccccgccgctggcggcgattcagctgaacatgtttaaatgcggcggcctggtgattggcgcgcagtttaaccatattattggcgatatgtttaccatgagcacctttatgaacagctgggcgaaagcgtgccgcgtgggcattaaagaagtggcgcatccgacctttggcctggcgccgctgatgccgagcgcgaaagtgctgaacattccgccgccgccgagctttgaaggcgtgaaatttgtgagcaaacgctttgtgtttaacgaaaacgcgattacccgcctgcgcaaagaagcgaccgaagaagatggcgatggcgatgatgatcagaaaaaaaaacgcccgagccgcgtggatctggtgaccgcgtttctgagcaaaagcctgattgaaatggattgcgcgaaaaaagaacagaccaaaagccgcccgagcctgatggtgcatatgatgaacctgcgcaaacgcaccaaactggcgctggaaaacgatgtgagcggcaacttttttattgtggtgaacgcggaaagcaaaattaccgtggcgccgaaaattaccgatctgaccgaaagcctgggcagcgcgtgcggcgaaattattagcgaagtggcgaaagtggatgatgcggaagtggtgagcagcatggtgctgaacagcgtgcgcgaattttattatgaatggggcaaaggcgaaaaaaacgtgtttctgtataccagctggtgccgctttccgctgtatgaagtggattttggctggggcattccgagcctggtggataccaccgcggtgccgtttggcctgattgtgctgatggatgaagcgccggcgggcgatggcattgcggtgcgcgcgtgcctgagcgaacatgatatgattcagtttcagcagcatcatcagctgctgagctatgtgagc BBa_M36915_sequence 1 aacgtgaccatgcatagcaaaaaactgctgaaaccgagcattccgaccccgaaccatctgcagaaactgaacctgagcctgctggatcagattcagattccgttttatgtgggcctgatttttcattatgaaaccctgagcgataacagcgatattaccctgagcaaactggaaagcagcctgagcgaaaccctgaccctgtattatcatgtggcgggccgctataacggcaccgattgcgtgattgaatgcaacgatcagggcattggctatgtggaaaccgcgtttgatgtggaactgcatcagtttctgctgggcgaagaaagcaacaacctggatctgctggtgggcctgagcggctttctgagcgaaaccgaaaccccgccgctggcggcgattcagctgaacatgtttaaatgcggcggcctggtgattggcgcgcagtttaaccatattattggcgatatgtttaccatgagcacctttatgaacagctgggcgaaagcgtgccgcgtgggcattaaagaagtggcgcatccgacctttggcctggcgccgctgatgccgagcgcgaaagtgctgaacattccgccgccgccgagctttgaaggcgtgaaatttgtgagcaaacgctttgtgtttaacgaaaacgcgattacccgcctgcgcaaagaagcgaccgaagaagatggcgatggcgatgatgatcagaaaaaaaaacgcccgagccgcgtggatctggtgaccgcgtttctgagcaaaagcctgattgaaatggattgcgcgaaaaaagaacagaccaaaagccgcccgagcctgatggtgcatatgatgaacctgcgcaaacgcaccaaactggcgctggaaaacgatgtgagcggcaacttttttattgtggtgaacgcggaaagcaaaattaccgtggcgccgaaaattaccgatctgaccgaaagcctgggcagcgcgtgcggcgaaattattagcgaagtggcgaaagtggatgatgcggaagtggtgagcagcatggtgctgaacagcgtgcgcgaattttattatgaatggggcaaaggcgaaaaaaacgtgtttctgtataccagctggtgccgctttccgctgtatgaagtggattttggctggggcattccgagcctggtggataccaccgcggtgccgtttggcctgattgtgctgatggatgaagcgccggcgggcgatggcattgcggtgcgcgcgtgcctgagcgaacatgatatgattcagtttcagcagcatcatcagctgctgagctatgtgagc BBa_M36913_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgggggagcgtttctgatg BBa_M36912_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z