BBa_M36933 1 BBa_M36933 P_rhaBAD 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 A strong promoter. false false _848_ 0 24151 9 Not in stock false None false Emily Kelly BBa_M36120 1 BBa_M36120 5' Bicistronic UTR (strong) contains ATG start Codon. 2013-10-24T11:00:00Z 2015-05-08T01:14:03Z This is based on research done in the paper "Precise and reliable gene expression via standard transcription and translation initiation elements." Used in place of an RBS. This device contains two RBS's to provide more reliability in gene selection. Place upsteam of your gene of interest in an actuator design false false _848_ 0 19058 9 Not in stock false We had to consider the strength in the design of this sequence. Because our protein ( human myoglobin) is not shown to be toxic to e. coli, we want in to be expressed in high numbers and thus we chose a strong BCD. false Alaina Shumate annotation2370704 1 misc range2370704 1 1 88 BBa_M36931 1 BBa_M36931 E. coli enoyl-[acyl-carrier-protien] reductase 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z Please see http://prodomweb.univ-lyon1.fr/priam/cgi-bin/ReqPRIAM_png.pl?priam_release=FEB14&priam_id=107psg650_1.3.1.9 for AA sequence. As the final reductive stage of fatty acid biosynthesis, enoyl-[acyl-carrier-protien] catalyses the following reaction: acyl-[acyl-carrier protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+. Enoyl-[acyl-carrier-protein] reductase (NADH). (EC 1.3.1.9). 107psg650_1.3.1.9. false false _848_ 0 24151 9 Not in stock false No BsaI, BbsI/BsmBI sites. No palindromes >= 10bp. false Emily Kelly BBa_M36932 1 BBa_M36932 Element 2 (Unidentified DNA from pD864: 954 to 1064) 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 An optimizer for E. coli plasmid replication. false false _848_ 0 24151 9 Not in stock false None false Emily Kelly BBa_M36117 1 BBa_M36117 Stop Codon 2012-05-28T11:00:00Z 2015-05-08T01:14:03Z NA Stop Codon false false _848_ 0 13354 9 Not in stock false NA false Samson Phan, Noriko Ishisoko BBa_M36938 1 BBa_M36938 Enoyl-[acyl-carrier-protien] reductase actuator with GFP reporter 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z Composite. A construct designed to produce Enoyl-[acyl-carrier-protien] reductase and GFP in E. coli. false false _848_ 0 24151 9 Not in stock true No BsaI, BbsI/BsmBI sites. No palindromes >= 10bp. false Emily Kelly component2428962 1 BBa_M36117 component2428963 1 BBa_M36934 component2428961 1 BBa_M36936 component2428959 1 BBa_M36120 component2428960 1 BBa_M36931 component2428956 1 BBa_M36932 component2428964 1 BBa_M36935 component2428957 1 BBa_M36933 annotation2428961 1 BBa_M36936 range2428961 1 710 982 annotation2428962 1 BBa_M36117 range2428962 1 991 993 annotation2428963 1 BBa_M36934 range2428963 1 1002 1014 annotation2428959 1 BBa_M36120 range2428959 1 242 329 annotation2428960 1 BBa_M36931 range2428960 1 336 701 annotation2428964 1 BBa_M36935 range2428964 1 1023 1113 annotation2428956 1 BBa_M36932 range2428956 1 1 111 annotation2428957 1 BBa_M36933 range2428957 1 120 233 BBa_M36936 1 BBa_M36936 CometGFP 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 GFP reporter sequence recommended by DNA 2.0. false false _848_ 0 24151 9 Not in stock false None false Emily Kelly BBa_M36935 1 BBa_M36935 Term_PhageT7 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 Terminator recommended by DNA 2.0. false false _848_ 0 24151 9 Not in stock false None. false Emily Kelly BBa_M36934 1 BBa_M36934 Element 7 (Unidentified DNA from pD864: 2761 to 2773) 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 Human linker sequence added for optimization purposes. false false _848_ 0 24151 9 Not in stock false None false Emily Kelly BBa_M36932_sequence 1 gccgatcaagaagacggtcaaaagcctccggtcggaggcttttgactttctgctatggaggtcaggtatgatttaaatggtcagtattgagcgatatctagagaattcgtc BBa_M36935_sequence 1 accgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccgggtaac BBa_M36117_sequence 1 taa BBa_M36931_sequence 1 atgcgcggtagcagcctgctgaccctgacttatctgggtccggaacgtgtgattccgaattacaacgttatgggtctggcaaaggccagcttggagagcagcgtccgttacctggccagcgacgcgggtaaagagggcatccgcagcaacgcgatttccgctggtccgatccgtacgctggcggcatctggtggcggcggcgacattggtggcgactttcgcctgctgctggaaggctccgagcagcgtgcgccactgcgttggaatctggtgaccagcacgccggtcggtaacaccgcagcgttcctgttgtcggatctgagcccgggtattaccggcatcatctatgttgatagcggttacatg BBa_M36938_sequence 1 gccgatcaagaagacggtcaaaagcctccggtcggaggcttttgactttctgctatggaggtcaggtatgatttaaatggtcagtattgagcgatatctagagaattcgtctactagagcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaattcaggcgctttttagactggtactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagatgcgcggtagcagcctgctgaccctgacttatctgggtccggaacgtgtgattccgaattacaacgttatgggtctggcaaaggccagcttggagagcagcgtccgttacctggccagcgacgcgggtaaagagggcatccgcagcaacgcgatttccgctggtccgatccgtacgctggcggcatctggtggcggcggcgacattggtggcgactttcgcctgctgctggaaggctccgagcagcgtgcgccactgcgttggaatctggtgaccagcacgccggtcggtaacaccgcagcgttcctgttgtcggatctgagcccgggtattaccggcatcatctatgttgatagcggttacatgtactagaggcgtgctgcggctgcaccggcgcgggctgcgcggcgaccgcggcgtgcaccgcgggctgcgcgaccgcggcgtgctgctgctgcaccaccggcggcggcggctgctgcacctgcaccgcggcggcgtgcggcggcggcacctgcaccaccggcgcgggcggcggcggcaccaccaccaccaccaccggctgcaccggcgcggcggcgggcggcgcgggcggcgcggcgtgcaccgcgaccgcgacctgctgcggcggcggcaccgcggcgtgctactagagtaatactagagtagagcggccgcctactagagaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccgggtaac BBa_M36933_sequence 1 caccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaattcaggcgctttttagactgg BBa_M36934_sequence 1 tagagcggccgcc BBa_M36936_sequence 1 gcgtgctgcggctgcaccggcgcgggctgcgcggcgaccgcggcgtgcaccgcgggctgcgcgaccgcggcgtgctgctgctgcaccaccggcggcggcggctgctgcacctgcaccgcggcggcgtgcggcggcggcacctgcaccaccggcgcgggcggcggcggcaccaccaccaccaccaccggctgcaccggcgcggcggcgggcggcgcgggcggcgcggcgtgcaccgcgaccgcgacctgctgcggcggcggcaccgcggcgtgc BBa_M36120_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z