BBa_M36939 1 BBa_M36939 Enoyl-[acyl-carrier-protien] reductase actuator 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z Composite. A construct designed to produce Enoyl-[acyl-carrier-protien] reductase in E. coli. false false _848_ 0 24151 9 Not in stock true No BsaI, BbsI/BsmBI sites. No palindromes >= 10bp. false Emily Kelly component2428978 1 BBa_M36117 component2428974 1 BBa_M36933 component2428979 1 BBa_M36934 component2428973 1 BBa_M36932 component2428976 1 BBa_M36120 component2428977 1 BBa_M36931 component2428980 1 BBa_M36935 annotation2428980 1 BBa_M36935 range2428980 1 742 832 annotation2428979 1 BBa_M36934 range2428979 1 721 733 annotation2428973 1 BBa_M36932 range2428973 1 1 111 annotation2428974 1 BBa_M36933 range2428974 1 120 233 annotation2428976 1 BBa_M36120 range2428976 1 242 329 annotation2428978 1 BBa_M36117 range2428978 1 710 712 annotation2428977 1 BBa_M36931 range2428977 1 336 701 BBa_M36933 1 BBa_M36933 P_rhaBAD 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 A strong promoter. false false _848_ 0 24151 9 Not in stock false None false Emily Kelly BBa_M36931 1 BBa_M36931 E. coli enoyl-[acyl-carrier-protien] reductase 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z Please see http://prodomweb.univ-lyon1.fr/priam/cgi-bin/ReqPRIAM_png.pl?priam_release=FEB14&priam_id=107psg650_1.3.1.9 for AA sequence. As the final reductive stage of fatty acid biosynthesis, enoyl-[acyl-carrier-protien] catalyses the following reaction: acyl-[acyl-carrier protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+. Enoyl-[acyl-carrier-protein] reductase (NADH). (EC 1.3.1.9). 107psg650_1.3.1.9. false false _848_ 0 24151 9 Not in stock false No BsaI, BbsI/BsmBI sites. No palindromes >= 10bp. false Emily Kelly BBa_M36932 1 BBa_M36932 Element 2 (Unidentified DNA from pD864: 954 to 1064) 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 An optimizer for E. coli plasmid replication. false false _848_ 0 24151 9 Not in stock false None false Emily Kelly BBa_M36117 1 BBa_M36117 Stop Codon 2012-05-28T11:00:00Z 2015-05-08T01:14:03Z NA Stop Codon false false _848_ 0 13354 9 Not in stock false NA false Samson Phan, Noriko Ishisoko BBa_M36120 1 BBa_M36120 5' Bicistronic UTR (strong) contains ATG start Codon. 2013-10-24T11:00:00Z 2015-05-08T01:14:03Z This is based on research done in the paper "Precise and reliable gene expression via standard transcription and translation initiation elements." Used in place of an RBS. This device contains two RBS's to provide more reliability in gene selection. Place upsteam of your gene of interest in an actuator design false false _848_ 0 19058 9 Not in stock false We had to consider the strength in the design of this sequence. Because our protein ( human myoglobin) is not shown to be toxic to e. coli, we want in to be expressed in high numbers and thus we chose a strong BCD. false Alaina Shumate annotation2370704 1 misc range2370704 1 1 88 BBa_M36935 1 BBa_M36935 Term_PhageT7 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 Terminator recommended by DNA 2.0. false false _848_ 0 24151 9 Not in stock false None. false Emily Kelly BBa_M36934 1 BBa_M36934 Element 7 (Unidentified DNA from pD864: 2761 to 2773) 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 Human linker sequence added for optimization purposes. false false _848_ 0 24151 9 Not in stock false None false Emily Kelly BBa_M36932_sequence 1 gccgatcaagaagacggtcaaaagcctccggtcggaggcttttgactttctgctatggaggtcaggtatgatttaaatggtcagtattgagcgatatctagagaattcgtc BBa_M36935_sequence 1 accgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccgggtaac BBa_M36117_sequence 1 taa BBa_M36931_sequence 1 atgcgcggtagcagcctgctgaccctgacttatctgggtccggaacgtgtgattccgaattacaacgttatgggtctggcaaaggccagcttggagagcagcgtccgttacctggccagcgacgcgggtaaagagggcatccgcagcaacgcgatttccgctggtccgatccgtacgctggcggcatctggtggcggcggcgacattggtggcgactttcgcctgctgctggaaggctccgagcagcgtgcgccactgcgttggaatctggtgaccagcacgccggtcggtaacaccgcagcgttcctgttgtcggatctgagcccgggtattaccggcatcatctatgttgatagcggttacatg BBa_M36933_sequence 1 caccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaattcaggcgctttttagactgg BBa_M36934_sequence 1 tagagcggccgcc BBa_M36939_sequence 1 gccgatcaagaagacggtcaaaagcctccggtcggaggcttttgactttctgctatggaggtcaggtatgatttaaatggtcagtattgagcgatatctagagaattcgtctactagagcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaattcaggcgctttttagactggtactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagatgcgcggtagcagcctgctgaccctgacttatctgggtccggaacgtgtgattccgaattacaacgttatgggtctggcaaaggccagcttggagagcagcgtccgttacctggccagcgacgcgggtaaagagggcatccgcagcaacgcgatttccgctggtccgatccgtacgctggcggcatctggtggcggcggcgacattggtggcgactttcgcctgctgctggaaggctccgagcagcgtgcgccactgcgttggaatctggtgaccagcacgccggtcggtaacaccgcagcgttcctgttgtcggatctgagcccgggtattaccggcatcatctatgttgatagcggttacatgtactagagtaatactagagtagagcggccgcctactagagaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccgggtaac BBa_M36120_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z