LOCUS       BBa_M36969              1535 bp    DNA     linear       23-Dec-2024
DEFINITION  PoPS->SMTa Metallothionein(should be BBa_M36968
ACCESSION   BBa_M36969
VERSION     BBa_M36969.1  
FEATURES             Location/Qualifiers
     RBS             19..27
                     /label=leader RBS
     misc_feature    33..35
                     /label=Start
     misc_feature    86..88
                     /label=Start
     misc_feature    84..86
                     /label=Stop
     misc_feature    71..79
                     /label=GOI RBS
     misc_feature    1..88
                     /label=BBa_J119025
     misc_feature    296..493
                     /label=BBa_K174009 (cotC)
     CDS             493..1204
                     /label=Gfp without start codon
     misc_feature    1205..1372
                     /label=BBa_K174019 (smtA without start codon)
     misc_feature    1373..1406
                     /label=terminator
     promoter        89..295
                     /label=sigK promoter + rbs
     misc_feature    89..1406
                     /label=BBa_K174008
     stem_loop       1418..1461
                     /label=stem_loop
     misc_feature    1407..1486
                     /label=BBa_B0010
     terminator      1407..1486
                     /label=BBa_B0010
     stem_loop       1502..1521
                     /label=T7 TE
     misc_feature    1528..1528
                     /label=stop
     polyA_site      1522..1535
                     /label=polya
     misc_feature    1495..1535
                     /label=BBa_B0012
     terminator      1495..1535
                     /label=BBa_B0012
     terminator      1407..1535
                     /label=BBa_B0015
ORIGIN
        1 gggcccaagt tcacttaaaa aggagatcaa caatgaaagc aattttcgta ctgaaacatc
       61 ttaatcatgc taaggaggtt ttctaatgga ctcagaaaac aaagaagaca aacaaatgta
      121 ggataaatcg tttgggccga tgaaaaatcg gctctttatt ttgatttgtt tttgtgtcat
      181 ctgtcttttt ctatcatttg gacagccctt ttttccttct atgattttaa ctgtccaagc
      241 cgcaaaatct actcgccgta taataaagcg tagtaaaaat aaaggaggag tatatatggg
      301 ttattacaaa aaatacaaag aagagtatta tacggtcaaa aaaacgtatt ataagaagta
      361 ttacgaatat gataaaaaag attatgactg tgattacgac aaaaaatatg atgactatga
      421 taaaaaatat tatgatcacg ataaaaaaga ctatgattat gttgtagagt ataaaaagca
      481 taaaaaacac taccgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      541 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      601 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      661 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
      721 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      781 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      841 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      901 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
      961 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     1021 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     1081 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     1141 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1201 caaaacctca acaacgttgg tcaaatgcgc ttgtgagccc tgtctctgca acgtcgatcc
     1261 cagcaaagcg atcgatcgca acggtctgta ctacttcagc gaagcctgtg ccgatggcca
     1321 caccggtggt agcaaaggct gcggccacac cggctgtaac tgccacggct aacgcaaaaa
     1381 accccgcttc ggcggggttt tttcgcccag gcatcaaata aaacgaaagg ctcagtcgaa
     1441 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1501 tggctcacct tcgggtgggc ctttctgcgt ttata
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