##gff-version 3
##sequence-region BBa_M36969 1 1535
BBa_M36969	.	terminator	1407	1535	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M36969	.	terminator	1495	1535	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M36969	.	polyA_site	1522	1535	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M36969	.	stem_loop	1502	1521	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M36969	.	stop_codon	1528	1528	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M36969	.	engineered_region	1495	1535	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M36969	.	terminator	1407	1486	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M36969	.	stem_loop	1418	1461	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M36969	.	engineered_region	1407	1486	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M36969	.	engineered_region	1	88	.	+	0	ID=BBa_J119025;Name=BBa_J119025
BBa_M36969	.	stop_codon	84	86	.	+	0	ID=annotation2167448;Name=Stop;Parent=BBa_J119025
BBa_M36969	.	start_codon	86	88	.	+	0	ID=annotation2167447;Name=Start;Parent=BBa_J119025
BBa_M36969	.	start_codon	33	35	.	+	0	ID=annotation2167446;Name=Start;Parent=BBa_J119025
BBa_M36969	.	sequence_feature	71	79	.	+	0	ID=annotation2167449;Name=GOI RBS;Parent=BBa_J119025
BBa_M36969	.	ribosome_entry_site	19	27	.	+	0	ID=annotation2167445;Name=leader RBS;Parent=BBa_J119025
BBa_M36969	.	engineered_region	89	1406	.	+	0	ID=BBa_K174008;Name=BBa_K174008
BBa_M36969	.	sequence_feature	1373	1406	.	+	0	ID=annotation2033737;Name=terminator;Parent=BBa_K174008
BBa_M36969	.	engineered_region	1205	1372	.	+	0	ID=annotation2033736;Name=BBa_K174019 (smtA without start codon);Parent=BBa_K174008
BBa_M36969	.	promoter	89	295	.	+	0	ID=annotation2033738;Name=sigK promoter + rbs;Parent=BBa_K174008
BBa_M36969	.	CDS	493	1204	.	+	0	ID=annotation2033735;Name=Gfp without start codon;Parent=BBa_K174008
BBa_M36969	.	engineered_region	296	493	.	+	0	ID=annotation2033731;Name=BBa_K174009 (cotC);Parent=BBa_K174008
>BBa_M36969
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggt
tttctaatggactcagaaaacaaagaagacaaacaaatgtaggataaatcgtttgggccgatgaaaaatcggctcttta
ttttgatttgtttttgtgtcatctgtctttttctatcatttggacagcccttttttccttctatgattttaactgtcca
agccgcaaaatctactcgccgtataataaagcgtagtaaaaataaaggaggagtatatatgggttattacaaaaaatac
aaagaagagtattatacggtcaaaaaaacgtattataagaagtattacgaatatgataaaaaagattatgactgtgatt
acgacaaaaaatatgatgactatgataaaaaatattatgatcacgataaaaaagactatgattatgttgtagagtataa
aaagcataaaaaacactaccgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgat
gttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttattt
gcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagata
cccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc
aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaag
gtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcat
ggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagca
gaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctg
ccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatgg
catggatgaactatacaaaacctcaacaacgttggtcaaatgcgcttgtgagccctgtctctgcaacgtcgatcccagc
aaagcgatcgatcgcaacggtctgtactacttcagcgaagcctgtgccgatggccacaccggtggtagcaaaggctgcg
gccacaccggctgtaactgccacggctaacgcaaaaaaccccgcttcggcggggttttttcgcccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata