BBa_K174008
1
BBa_K174008
SmtA metallothionein protein with CotC and Gfp fusion
2009-10-09T11:00:00Z
2015-05-08T01:10:58Z
smtA sequence is taken from ''E. coli''
Metallothionein protein can bind to heavy metals such as cadmium, arsenic, mercury, silver. By fusing CotC spore coat protein from ''Bacillus subtilis'', it can be localized to the spore coat, hence trap the metals into bacterial spores. It is also fused with Gfp to see the spores with the proteins using a microscope.
false
false
_277_
0
3942
9
It's complicated
true
It is integrated to ''B. subtilis'' using the homology between cotC fusion and the native copy of cotC on the chromosome.
false
The Newcastle 2009 iGEM team
annotation2033738
1
sigK promoter + rbs
range2033738
1
1
207
annotation2033735
1
Gfp without start codon
range2033735
1
405
1116
annotation2033737
1
terminator
range2033737
1
1285
1318
annotation2033731
1
BBa_K174009 (cotC)
range2033731
1
208
405
annotation2033736
1
BBa_K174019 (smtA without start codon)
range2033736
1
1117
1284
BBa_M36969
1
ElChu
PoPS->SMTa Metallothionein(should be BBa_M36968
2012-12-06T12:00:00Z
2015-05-08T01:14:07Z
THe SMTa gene comes from e.Coli
Takes in a PoPS signal, and expresses a SMTa Metallothionein copper sequestering protein
false
false
_848_
0
15039
9
Not in stock
false
We made some modifications to the genetic code to delete repeated sequences. We also manually inputed a termination sequence prior to the Transcription Terminator as a secondary terminator.
false
Dan Nelsen, Shivani Torres, Aaron Goebel
component2213650
1
BBa_J119025
component2213656
1
BBa_K174008
component2213663
1
BBa_B0015
annotation2213656
1
BBa_K174008
range2213656
1
89
1406
annotation2213650
1
BBa_J119025
range2213650
1
1
88
annotation2213663
1
BBa_B0015
range2213663
1
1407
1535
BBa_J119025
1
BBa_J119025
BD2 bicistronic translational junction - Lowest efficiency
2011-12-21T12:00:00Z
2015-10-01T11:42:55Z
Synthetic DNA
The BD2 bicistronic translational junction was engineered by Vivek Mutalik and The BIOFAB Team at biofab.org. It includes an RBS that allows translation of a leader polypeptide. The leader has a second RBS embedded in it. The stop codon for the leader polypeptide overlaps with the start codon for the gene of interest.
false
false
_613_
606
606
61
Not in stock
false
The bicistronic translational junction was designed by Team Leader Vivek Mutalik at BioFAB. It is is intended to give reproducible and consistent expression for various genes of interest.
false
Vivek Mutalik
annotation2167446
1
Start
range2167446
1
33
35
annotation2167447
1
Start
range2167447
1
86
88
annotation2167445
1
leader RBS
range2167445
1
19
27
annotation2167448
1
Stop
range2167448
1
84
86
annotation2167449
1
GOI RBS
range2167449
1
71
79
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J119025_sequence
1
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_M36969_sequence
1
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatggactcagaaaacaaagaagacaaacaaatgtaggataaatcgtttgggccgatgaaaaatcggctctttattttgatttgtttttgtgtcatctgtctttttctatcatttggacagcccttttttccttctatgattttaactgtccaagccgcaaaatctactcgccgtataataaagcgtagtaaaaataaaggaggagtatatatgggttattacaaaaaatacaaagaagagtattatacggtcaaaaaaacgtattataagaagtattacgaatatgataaaaaagattatgactgtgattacgacaaaaaatatgatgactatgataaaaaatattatgatcacgataaaaaagactatgattatgttgtagagtataaaaagcataaaaaacactaccgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaacctcaacaacgttggtcaaatgcgcttgtgagccctgtctctgcaacgtcgatcccagcaaagcgatcgatcgcaacggtctgtactacttcagcgaagcctgtgccgatggccacaccggtggtagcaaaggctgcggccacaccggctgtaactgccacggctaacgcaaaaaaccccgcttcggcggggttttttcgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K174008_sequence
1
gactcagaaaacaaagaagacaaacaaatgtaggataaatcgtttgggccgatgaaaaatcggctctttattttgatttgtttttgtgtcatctgtctttttctatcatttggacagcccttttttccttctatgattttaactgtccaagccgcaaaatctactcgccgtataataaagcgtagtaaaaataaaggaggagtatatatgggttattacaaaaaatacaaagaagagtattatacggtcaaaaaaacgtattataagaagtattacgaatatgataaaaaagattatgactgtgattacgacaaaaaatatgatgactatgataaaaaatattatgatcacgataaaaaagactatgattatgttgtagagtataaaaagcataaaaaacactaccgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaacctcaacaacgttggtcaaatgcgcttgtgagccctgtctctgcaacgtcgatcccagcaaagcgatcgatcgcaacggtctgtactacttcagcgaagcctgtgccgatggccacaccggtggtagcaaaggctgcggccacaccggctgtaactgccacggctaacgcaaaaaaccccgcttcggcggggttttttcgc
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z