LOCUS       BBa_M36979              1311 bp    DNA     linear       17-May-2024
DEFINITION  Coenzyme B-12 Biosensor for E. coli (
ACCESSION   BBa_M36979
VERSION     BBa_M36979.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             245..272
                     /label=Shine-Dalgarno Sequence
     misc_feature    177..194
                     /label=B12 box/B12 element
     misc_feature    36..275
                     /label=BBa_M36998
     CDS             276..455
                     /label=lacZa
     misc_feature    456..512
                     /label=linker
     CDS             513..1229
                     /label=GFP
     misc_feature    276..1229
                     /label=BBa_E0051
     terminator      1230..1311
                     /label=BBa_M36010
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagcgccgg tcctgtgagt taatagggaa
       61 tccagtgcga atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg
      121 cggacactgc cattcggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc
      181 ccgaagacct gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac
      241 ctgcggcatc cttcttctat tgtggatgct ttacaatgac catgattacg gattcactgg
      301 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      361 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      421 cccaacagtt gcgcagcctg aatggcgaat ggcgcgcggg cggcagcgaa ggcggcggca
      481 gcgaacatca tcatcatcat catggcagcg aacgtaaagg agaagaactt ttcactggag
      541 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      601 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      661 gaaaactacc tgttccatgg ccaacacttg tcactaccct gacctatggt gttcaatgct
      721 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
      781 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
      841 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
      901 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
      961 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     1021 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     1081 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     1141 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     1201 atggcatgga tgaactatac aaataataat cggtcagttt cacctgattt acgtaaaaac
     1261 ccgcttcggc gggtttttgc ttttggaggg gcagaaagat gaatgactgt c
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