BBa_M36979 1 BBa_M36979 Coenzyme B-12 Biosensor for E. coli ( 2011-05-02T11:00:00Z 2015-05-08T01:14:07Z d Composite part false false _848_ 0 9648 9 Not in stock false d false Nick Davis, Rachel RoseFigura component2118876 1 BBa_J23100 component2118877 1 BBa_M36998 component2118881 1 BBa_E0051 component2118882 1 BBa_M36010 annotation2118877 1 BBa_M36998 range2118877 1 36 275 annotation2118881 1 BBa_E0051 range2118881 1 276 1229 annotation2118876 1 BBa_J23100 range2118876 1 1 35 annotation2118882 1 BBa_M36010 range2118882 1 1230 1311 BBa_E0051 1 BBa_E0051 lacZa.GFP fusion 2009-03-01T12:00:00Z 2015-08-31T04:07:25Z amino acids 3-60 from lacZ and amino acids 2-end of GFP (E0043) This part is a fusion protein between GFP and the alpha fragment of lacZ. It can be measured via fluorescence from GFP or through an assay for beta-galactosidase in a strain containing the omega fragment of lacZ. false false _41_ 0 22 84 Not in stock false The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc. false Austin Che annotation2001727 1 linker range2001727 1 181 237 annotation2001726 1 lacZa range2001726 1 1 180 annotation2001728 1 GFP range2001728 1 238 954 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_M36998 1 BBa_M36998 Coenzyme B-12 Riboswitch for E. coli (nucleotides 1-240) 2011-04-27T11:00:00Z 2015-05-08T01:14:07Z Naturally-occurring, 240-nucleotides upstream of the btuB (B-12 uptake) coding sequence in E. coli. BtuB (the protein coded by the btuB gene) is a B-12 membrane transporter in E. coli bacteria. This part contains the full string of nucleotides for the coenzyme B-12 riboswitch present in Escherichia coli. The aptamer domain of the riboswitch (nucleotides 1-202) undergoes substantial allosteric changes upon binding coenzyme B-12 (aka hydroxocobalamin). The coenzyme B-12 aptamer has one of the most complex metabolite-binding structures of known naturally-occurring riboswitches, though other riboswitches with simpler aptamers can also sense their targets with impressive precision and use the resultant structural changes to control gene expression. Nucleotides 203-240 represent the 5'UTR (untranslated region) upstream of the btuB coding sequence in E. coli genome. false false _848_ 0 9648 9 Not in stock false An executive decision had to be made to include the 5'UTR region following the cobalamin ribowitch (aptamer) sequence. Nucleotides 203-240 represent the 5'UTR upstream of the btuB coding sequence. We chose to include these nucleotides in our part, as it is not completely clear what distinct role this region plays in translational control by the riboswitch, and few studies have demonstrated the significance of this region. false Nick Davis, Rachel RoseFigura annotation2119205 1 B12 box/B12 element range2119205 1 142 159 annotation2119161 1 Shine-Dalgarno Sequence range2119161 1 210 237 BBa_M36010 1 BBa_M36010 Transcription Terminator (Strong) 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z Guillaume Cambray et al. c/o BIOFAB Emeryville E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator. false true _848_ 0 52 432 Not in stock false please see long description false Drew Endy BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_E0051_sequence 1 atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_M36010_sequence 1 tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_M36998_sequence 1 gccggtcctgtgagttaatagggaatccagtgcgaatctggagctgacgcgcagcggtaaggaaaggtgcgatgattgcgttatgcggacactgccattcggtgggaagtcatcatctcttagtatcttagatacccctccaagcccgaagacctgccggccaacgtcgcatctggttctcatcatcgcgtaatattgatgaaacctgcggcatccttcttctattgtggatgctttaca BBa_M36979_sequence 1 ttgacggctagctcagtcctaggtacagtgctagcgccggtcctgtgagttaatagggaatccagtgcgaatctggagctgacgcgcagcggtaaggaaaggtgcgatgattgcgttatgcggacactgccattcggtgggaagtcatcatctcttagtatcttagatacccctccaagcccgaagacctgccggccaacgtcgcatctggttctcatcatcgcgtaatattgatgaaacctgcggcatccttcttctattgtggatgctttacaatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z