BBa_M36980 1 BBa_M36980 Coenzyme B-12 Biosensor for E. coli ( 2011-05-02T11:00:00Z 2015-05-08T01:14:07Z Designed by Nick Davis and Rachel RoseFigura, Bioengineering undergraduate students at Stanford University. Composite part for a Vitamin B12 Biosensor for Escherichia coli. Features a constitutively-active promoter (~99% promotion efficiency), the naturally-occurring vitamin B-12 riboswitch sequence, gemini fusion protein coding sequence (coding for both GFP and ??-galactosidase production), and a strong promoter (~99% termination efficiency). true false _848_ 0 9648 9 Discontinued false As opposed to more "traditional" biological sensor and actuator parts (that operate on the principle of PoPs), this composite Vit B12 Biosensor relies on RiPS (Ribosomes per second), as the main sensing structure (part M36998) is the naturally-occurring vitamin B12 riboswitch???a length of RNA that forms complex aptamer structures upon binding of vitamin B12 as the substrate. false Nick Davis, Rachel RoseFigura component2118607 1 BBa_E0051 component2118608 1 BBa_M36010 component2118602 1 BBa_J23100 component2118603 1 BBa_M36998 annotation2118602 1 BBa_J23100 range2118602 1 1 35 annotation2118608 1 BBa_M36010 range2118608 1 1252 1333 annotation2118603 1 BBa_M36998 range2118603 1 44 283 annotation2118607 1 BBa_E0051 range2118607 1 290 1243 BBa_M36998 1 BBa_M36998 Coenzyme B-12 Riboswitch for E. coli (nucleotides 1-240) 2011-04-27T11:00:00Z 2015-05-08T01:14:07Z Naturally-occurring, 240-nucleotides upstream of the btuB (B-12 uptake) coding sequence in E. coli. BtuB (the protein coded by the btuB gene) is a B-12 membrane transporter in E. coli bacteria. This part contains the full string of nucleotides for the coenzyme B-12 riboswitch present in Escherichia coli. The aptamer domain of the riboswitch (nucleotides 1-202) undergoes substantial allosteric changes upon binding coenzyme B-12 (aka hydroxocobalamin). The coenzyme B-12 aptamer has one of the most complex metabolite-binding structures of known naturally-occurring riboswitches, though other riboswitches with simpler aptamers can also sense their targets with impressive precision and use the resultant structural changes to control gene expression. Nucleotides 203-240 represent the 5'UTR (untranslated region) upstream of the btuB coding sequence in E. coli genome. false false _848_ 0 9648 9 Not in stock false An executive decision had to be made to include the 5'UTR region following the cobalamin ribowitch (aptamer) sequence. Nucleotides 203-240 represent the 5'UTR upstream of the btuB coding sequence. We chose to include these nucleotides in our part, as it is not completely clear what distinct role this region plays in translational control by the riboswitch, and few studies have demonstrated the significance of this region. false Nick Davis, Rachel RoseFigura annotation2119205 1 B12 box/B12 element range2119205 1 142 159 annotation2119161 1 Shine-Dalgarno Sequence range2119161 1 210 237 BBa_E0051 1 BBa_E0051 lacZa.GFP fusion 2009-03-01T12:00:00Z 2015-08-31T04:07:25Z amino acids 3-60 from lacZ and amino acids 2-end of GFP (E0043) This part is a fusion protein between GFP and the alpha fragment of lacZ. It can be measured via fluorescence from GFP or through an assay for beta-galactosidase in a strain containing the omega fragment of lacZ. false false _41_ 0 22 84 Not in stock false The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc. false Austin Che annotation2001726 1 lacZa range2001726 1 1 180 annotation2001728 1 GFP range2001728 1 238 954 annotation2001727 1 linker range2001727 1 181 237 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_M36010 1 BBa_M36010 Transcription Terminator (Strong) 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z Guillaume Cambray et al. c/o BIOFAB Emeryville E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator. false true _848_ 0 52 432 Not in stock false please see long description false Drew Endy BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_M36980_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagaggccggtcctgtgagttaatagggaatccagtgcgaatctggagctgacgcgcagcggtaaggaaaggtgcgatgattgcgttatgcggacactgccattcggtgggaagtcatcatctcttagtatcttagatacccctccaagcccgaagacctgccggccaacgtcgcatctggttctcatcatcgcgtaatattgatgaaacctgcggcatccttcttctattgtggatgctttacatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagtcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_E0051_sequence 1 atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_M36010_sequence 1 tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_M36998_sequence 1 gccggtcctgtgagttaatagggaatccagtgcgaatctggagctgacgcgcagcggtaaggaaaggtgcgatgattgcgttatgcggacactgccattcggtgggaagtcatcatctcttagtatcttagatacccctccaagcccgaagacctgccggccaacgtcgcatctggttctcatcatcgcgtaatattgatgaaacctgcggcatccttcttctattgtggatgctttaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z