BBa_M39012 1 BBa_M39012 Sialic Acid Receptor 2012-05-12T11:00:00Z 2015-05-08T01:14:07Z lots of stuff This design will produce one of two necessary transcription factors for the HrpL AND gate. This will produce it in the presence of Sialic Acid false false _1206_ 0 13450 9 Not in stock false we wanted a Negative Feed Back loop false Edgar Aranda-Michel component2225403 1 BBa_J23100 component2225432 1 BBa_M39011 component2225428 1 BBa_M39007 component2225407 1 BBa_M39008 component2225415 1 BBa_M39009 component2225412 1 BBa_M39007 component2225423 1 BBa_M39010 annotation2225415 1 BBa_M39009 range2225415 1 1032 2522 annotation2225403 1 BBa_J23100 range2225403 1 1 35 annotation2225432 1 BBa_M39011 range2225432 1 5350 6394 annotation2225428 1 BBa_M39007 range2225428 1 5221 5341 annotation2225407 1 BBa_M39008 range2225407 1 44 896 annotation2225412 1 BBa_M39007 range2225412 1 905 1025 annotation2225423 1 BBa_M39010 range2225423 1 2531 5212 BBa_M39008 1 BBa_M39008 NanR 2012-05-12T11:00:00Z 2015-05-08T01:14:07Z E.Coli This codes for the NanR Regulator. This is the the repressor for the Nan Operon and is turned off by the presence of sialic acid. false false _1206_ 0 13450 9 Not in stock false n/a false Edgar Aranda-Michel annotation2174885 1 rbs range2174885 1 1 25 annotation2174887 1 Termintor range2174887 1 818 853 annotation2174886 1 NanR range2174886 1 26 817 BBa_M39010 1 BBa_M39010 Digestion Enzymes for Sialic Acids 2012-05-12T11:00:00Z 2015-05-08T01:14:07Z E.Coli These are the enzymes needed for Sialic Acid breakdown in E.Coli false false _1206_ 0 13450 9 Not in stock false Since these are all on an operon, we made sure there was sufficient space and an RBS in-between each coding region false Edgar Aranda-Michel annotation2174769 1 rbs range2174769 1 37 62 annotation2174775 1 NanK range2174775 1 1771 2646 annotation2174770 1 NanA range2174770 1 63 957 annotation2174773 1 rbs range2174773 1 1746 1770 annotation2174772 1 NanE range2174772 1 1019 1709 annotation2174776 1 Terminator range2174776 1 2647 2682 annotation2174771 1 rbs range2174771 1 994 1018 BBa_M39007 1 BBa_M39007 Promoter Sequence for NanR 2012-05-12T11:00:00Z 2015-05-08T01:14:07Z E.Coli Sequencing This is the promoter sequence for the NanR transcription factor in the Nan Operon. This Operon is for Sialic Acid Degradation. Promoter is for E.Coli false false _1206_ 0 13450 9 Not in stock false n/a false Edgar Aranda-Michel annotation2174767 1 +1 Site range2174767 1 78 78 annotation2174765 1 NanR Binding Site range2174765 1 64 69 annotation2174768 1 NanR Binding Site range2174768 1 81 86 annotation2174766 1 NanR Binding Site range2174766 1 73 78 BBa_M39011 1 BBa_M39011 HrpS Gene 2012-05-12T11:00:00Z 2015-05-08T01:14:07Z E.Coli This codes for one of the two necessary genes (HrpS and HrpR) that will be needed to activate the HrpL AND gate false false _1206_ 0 13450 9 Not in stock false n/a false Edgar Aranda-Michel annotation2174857 1 Terminator range2174857 1 1010 1045 annotation2174855 1 rbs range2174855 1 1 25 annotation2174856 1 HrpS range2174856 1 26 1009 BBa_M39009 1 BBa_M39009 NanT 2012-05-12T11:00:00Z 2015-05-08T01:14:07Z E. Coli This allows the transport of Sialic Acid into the cell false false _1206_ 0 13450 9 Not in stock false n/a false Edgar Aranda-Michel annotation2174853 1 rbs range2174853 1 1 25 annotation2174854 1 NanT range2174854 1 26 1491 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_M39008_sequence 1 tctagagaaagaggggacaaactagatgggccttatgaacgcatttgattcgcaaaccgaagattcttcacctgcaattggtcgcaacttgcgtagccgcccgctggcgcgtaaaaaactctccgaaatggtggaagaagagctggaacagatgatccgccgtcgtgaatttggcgaaggtgaacaattaccgtctgaacgcgaactgatggcgttctttaacgtcgggcgtccttcggtgcgtgaagcgctggcagcgttaaaacgcaaaggtctggtgcaaataaacaacggcgaacgcgctcgcgtctcgcgtccttctgcggacactatcatcggtgagctttccggcatggcgaaagatttcctttctcatcccggtgggattgcccatttcgaacaattacgtctgttctttgaatccagtctggtgcgctatgcggctgaacatgccaccgatgagcaaatcgatttgctggcaaaagcactggaaatcaacagtcagtcgctggataacaacgcggcattcattcgttcagacgttgatttccaccgcgtgctggcggagatccccggtaacccaatcttcatggcgatccacgttgccctgctcgactggcttattgccgcacgcccaacggttaccgatcaggcactgcacgaacataacaacgttagttatcaacagcatattgcgatcgttgatgcgatccgccgtcatgatcctgacgaagccgatcgtgcgttgcaatcgcatctcaacagcgtctctgctacctggcacgctttcggtcagaccaccaacaaaaagaaataaaaacggcgaagcttaagcttcgccgttttttttttc BBa_M39009_sequence 1 atgagtactacaacccagaatatcccgtggtatcgccatctcaaccgtgcacaatggcgcgcattttccgctgcctggttgggatatctgcttgacggttttgatttcgttttaatcgccctggtactcaccgaagtacaaggtgaattcgggctgacgacggtgcaggcggcaagtctgatctctgcagcctttatctctcgctggttcggcggcctgatgctcggcgctatgggtgaccgctacgggcgtcgtctggcaatggtcaccagcatcgttctcttctcggccgggacgctggcctgcggctttgcgccaggctacatcaccatgtttatcgctcgtctggtcatcggcatggggatggcgggtgaatacggttccagcgccacctatgtcattgaaagctggccaaaacatctgcgtaacaaagccagtggttttttgatttcaggcttctctgtgggggccgtcgttgccgctcaggtctatagcctggtggttccggtctggggctggcgtgcgctgttctttatcggcattttgccaatcatctttgctctctggctgcgtaaaaacatcccggaagcggaagactggaaagagaaacacgcaggtaaagcaccagtacgcacaatggtggatattctctaccgtggtgaacatcgcattgccaatatcgtaatgacactggcggcggctactgcgctgtggttctgcttcgccggtaacctgcaaaatgccgcgatcgtcgctgttcttgggctgttatgcgccgcaatctttatcagctttatggtgcagagtgcaggcaaacgctggccaacgggcgtaatgctgatggtggtcgtgttgtttgctttcctctactcatggccgattcaggcgctgctgccaacgtatctgaaaaccgatctggcttataacccgcatactgtagccaatgtgctgttctttagtggctttggcgcggcggtgggatgctgcgtaggtggcttcctcggtgactggctgggaacccgcaaagcgtacgtttgtagcctgctggcctcgcagctgctgattattccggtatttgcgattggcggcgcaaacgtctgggtgctcggtctgttactgttcttccagcaaatgcttggacaagggatcgccgggatcttaccaaaactgattggcggttatttcgataccgaccagcgtgcagcgggcctgggctttacctacaacgttggcgcattgggcggtgcactggccccaatcatcggcgcgttgatcgctcaacgtctggatctgggtactgcgctggcatcgctctcgttcagtctgacgttcgtggtgatcctgctgattgggctggatatgccttctcgcgttcagcgttggttgcgcccggaagcgttgcgtactcatgacgctatcgacggtaaaccattcagcggtgccgtgccgtttggcagcgccaaaaacgatttagtcaaaaccaaaagttaa BBa_M39011_sequence 1 tctagagaaagaggggacaaactagatgacgataaggaatagcaatgaatccgtgcagtcacttcattgtgcctcccatgagtcctcgttaacgcatccgtcggaagatatacacggatcgctatcttctctcatccacactatcgcgcccctcaacgttgatatcgtactggaaggcgaaaccggcaccggcaaagacacgctggcgcgccgtattcatcagctttccggctgcgccggcccgttggtggcggtgaactgcgccgccgtgccggaaacgctggcggaaagcgagttattcggggtggtgtccggcgcttacaccggcgccagccggtcccgcgccggataccttgaaaccgcggacaaaggcattctgtttctggatgagatcgacagtatgccgctgacgctgcaggccaaaatgttacgggttctggaaagtcgcggcatcgaacggctgggcagcacccaattcaaaccggtggatatgcgggtgattgtcgccacgcagacgccattgcagaaactggtggacgaagggcgttttcggcgtgacctctacttccggctggatacggtaaaaattcagctgccgacgctgcgttcgcgtacggaaatcattctgccgttattcgagcgctttttgctcgaggcctcggcccgcctgaaacggccggcgccgggcctgtcggccatcattcaggaacaactgctgatgcacggctggcccggcaacattcgtgagttgaaggcggcggccgagcgctgggtgctggggctgtcgccggtgccgatagccagtgatggcgacgtgcaggtaatgggagaggatacgccgctgacgtcgctgaaagtgcgcctgcgccgcattgagcggtttctgattcaggaagcgttgcagcgcaacgaccactgtatcgatacggtggtgaatgaactgggcattcccaaacgcacgctctatcaccgcatcaagctgctgaatgtggcggcgcgcgggctgtaaaaacggcgaagcttaagcttcgccgttttttttttc BBa_M39012_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagtctagagaaagaggggacaaactagatgggccttatgaacgcatttgattcgcaaaccgaagattcttcacctgcaattggtcgcaacttgcgtagccgcccgctggcgcgtaaaaaactctccgaaatggtggaagaagagctggaacagatgatccgccgtcgtgaatttggcgaaggtgaacaattaccgtctgaacgcgaactgatggcgttctttaacgtcgggcgtccttcggtgcgtgaagcgctggcagcgttaaaacgcaaaggtctggtgcaaataaacaacggcgaacgcgctcgcgtctcgcgtccttctgcggacactatcatcggtgagctttccggcatggcgaaagatttcctttctcatcccggtgggattgcccatttcgaacaattacgtctgttctttgaatccagtctggtgcgctatgcggctgaacatgccaccgatgagcaaatcgatttgctggcaaaagcactggaaatcaacagtcagtcgctggataacaacgcggcattcattcgttcagacgttgatttccaccgcgtgctggcggagatccccggtaacccaatcttcatggcgatccacgttgccctgctcgactggcttattgccgcacgcccaacggttaccgatcaggcactgcacgaacataacaacgttagttatcaacagcatattgcgatcgttgatgcgatccgccgtcatgatcctgacgaagccgatcgtgcgttgcaatcgcatctcaacagcgtctctgctacctggcacgctttcggtcagaccaccaacaaaaagaaataaaaacggcgaagcttaagcttcgccgttttttttttctactagagtgccactttagtgaagcagatcgcattataagctttctgtatggggtgttgcttaattgatctggtataacaggtataaaggtatatcgtttatcagacaagcatcacttcagaggtattttactagatgagtactacaacccagaatatcccgtggtatcgccatctcaaccgtgcacaatggcgcgcattttccgctgcctggttgggatatctgcttgacggttttgatttcgttttaatcgccctggtactcaccgaagtacaaggtgaattcgggctgacgacggtgcaggcggcaagtctgatctctgcagcctttatctctcgctggttcggcggcctgatgctcggcgctatgggtgaccgctacgggcgtcgtctggcaatggtcaccagcatcgttctcttctcggccgggacgctggcctgcggctttgcgccaggctacatcaccatgtttatcgctcgtctggtcatcggcatggggatggcgggtgaatacggttccagcgccacctatgtcattgaaagctggccaaaacatctgcgtaacaaagccagtggttttttgatttcaggcttctctgtgggggccgtcgttgccgctcaggtctatagcctggtggttccggtctggggctggcgtgcgctgttctttatcggcattttgccaatcatctttgctctctggctgcgtaaaaacatcccggaagcggaagactggaaagagaaacacgcaggtaaagcaccagtacgcacaatggtggatattctctaccgtggtgaacatcgcattgccaatatcgtaatgacactggcggcggctactgcgctgtggttctgcttcgccggtaacctgcaaaatgccgcgatcgtcgctgttcttgggctgttatgcgccgcaatctttatcagctttatggtgcagagtgcaggcaaacgctggccaacgggcgtaatgctgatggtggtcgtgttgtttgctttcctctactcatggccgattcaggcgctgctgccaacgtatctgaaaaccgatctggcttataacccgcatactgtagccaatgtgctgttctttagtggctttggcgcggcggtgggatgctgcgtaggtggcttcctcggtgactggctgggaacccgcaaagcgtacgtttgtagcctgctggcctcgcagctgctgattattccggtatttgcgattggcggcgcaaacgtctgggtgctcggtctgttactgttcttccagcaaatgcttggacaagggatcgccgggatcttaccaaaactgattggcggttatttcgataccgaccagcgtgcagcgggcctgggctttacctacaacgttggcgcattgggcggtgcactggccccaatcatcggcgcgttgatcgctcaacgtctggatctgggtactgcgctggcatcgctctcgttcagtctgacgttcgtggtgatcctgctgattgggctggatatgccttctcgcgttcagcgttggttgcgcccggaagcgttgcgtactcatgacgctatcgacggtaaaccattcagcggtgccgtgccgtttggcagcgccaaaaacgatttagtcaaaaccaaaagttaatactagagaagcagatcgcattataagctttctgtatggggtgtttctagagaaagaggggacaaactagatggcaacgaatttacgtggcgtaatggctgcactcctgactccttttgaccaacaacaagcactggataaagcgagtctgcgtcgcctggttcagttcaatattcagcagggcatcgacggtttatacgtgggtggttcgaccggcgaggcctttgtacaaagcctttccgagcgtgaacaggtactggaaatcgtcgccgaagaggcgaaaggtaagattaaactcatcgcccacgtcggttgcgtcagcaccgccgaaagccaacaacttgcggcatcggctaaacgttatggcttcgatgccgtctccgccgtcacgccgttctactatcctttcagctttgaagaacactgcgatcactatcgggcaattattgattcggcggatggtttgccgatggtggtgtacaacattccagccctgagtggggtaaaactgaccctggatcagatcaacacacttgttacattgcctggcgtaggtgcgctgaaacagacctctggcgatctctatcagatggagcagatccgtcgtgaacatcctgatcttgtgctctataacggttacgacgaaatcttcgcctctggtctgctggcgggcgctgatggtggtatcggcagtacctacaacatcatgggctggcgctatcaggggatcgttaaggcgctgaaagaaggcgatatccagaccgcgcagaaactgcaaactgaatgcaataaagtcattgatttactgatcaaaacgggcgtattccgcggcctgaaaactgtcctccattatatggatgtcgtttctgtgccgctgtgccgcaaaccgtttggaccggtagatgaaaaatatctgccagaactgaaggcgctggcccagcagttgatgcaagagcgcgggtgaaagcagatcgcattataagctttctgtatggggtgtttctagagaaagaggggacaaactagatgtcgttacttgcacaactggatcaaaaaatcgctgctaacggtggcctgattgtctcctgccagccggttccggacagcccgctcgataaacccgaaatcgtcgccgccatggcattagcggcagaacaggcgggcgcggttgccattcgcattgaaggtgtggcaaatctgcaagccacgcgtgcggtggtgagcgtgccgattattggaattgtgaaacgcgatctggaggattctccggtacgcatcacggcctatattgaagatgttgatgcgctggcgcaggcgggcgcggacattatcgccattgacggcaccgaccgcccgcgtccggtgcctgttgaaacgctgctggcacgtattcaccatcacggtttactggcgatgaccgactgctcaacgccggaagacggcctggcatgccaaaagctgggagccgaaattattggcactacgctttctggctataccacgcctgaaacgccagaagagccggatctggcgctggtgaaaacgttgagcgacgccggatgtcgggtgattgccgaagggcgttacaacacgcctgctcaggcggcggatgcgatgcgccacggcgcgtgggcggtgacggtcggttctgcaatcacgcgtcttgagcacatttgtcagtggtacaacacagcgatgaaaaaggcggtgctatgaaagcagatcgcattataagctttctgtatggggtgtttctagagaaagaggggacaaactagatgaccacactggcgattgatatcggcggtactaaacttgccgccgcgctgattggcgctgacgggcagatccgcgatcgtcgtgaacttcctacgccagccagccagacaccagaagccttgcgtgatgccttatccgcattagtctctccgttgcaagctcatgcgcagcgggttgccatcgcttcgaccgggataatccgtgacggcagcttgctggcgcttaatccgcataatcttggtggattgctacactttccgttagtcaaaacgctggaacaacttaccaatttgccgaccattgccattaacgacgcgcaggccgcagcatgggcggagtttcaggcgctggatggcgatataaccgatatggtctttatcaccgtttccaccggcgttggcggcggtgtagtgagcggctgcaaactgcttaccggccctggcggtctggcggggcatatcgggcatacgcttgccgatccacacggcccagtctgcggctgtggacgcacaggttgcgtggaagcgattgcttctggtcgcggcattgcagcggcagcgcagggggagttggctggcgcggatgcgaaaactattttcacgcgcgccgggcagggtgacgagcaggcgcagcagctgattcaccgctccgcacgtacgcttgcaaggctgatcgctgatattaaagccacaactgattgccagtgcgtggtggtcggtggcagcgttggtctggcagaagggtatctggcgctggtggaaacgtatctggcgcaggagccagcggcatttcatgttgatttactggcggcgcattaccgccatgatgcaggtttacttggggctgcgctgttggcccagggagaaaaattatgaaaacggcgaagcttaagcttcgccgttttttttttctactagagtgccactttagtgaagcagatcgcattataagctttctgtatggggtgttgcttaattgatctggtataacaggtataaaggtatatcgtttatcagacaagcatcacttcagaggtattttactagagtctagagaaagaggggacaaactagatgacgataaggaatagcaatgaatccgtgcagtcacttcattgtgcctcccatgagtcctcgttaacgcatccgtcggaagatatacacggatcgctatcttctctcatccacactatcgcgcccctcaacgttgatatcgtactggaaggcgaaaccggcaccggcaaagacacgctggcgcgccgtattcatcagctttccggctgcgccggcccgttggtggcggtgaactgcgccgccgtgccggaaacgctggcggaaagcgagttattcggggtggtgtccggcgcttacaccggcgccagccggtcccgcgccggataccttgaaaccgcggacaaaggcattctgtttctggatgagatcgacagtatgccgctgacgctgcaggccaaaatgttacgggttctggaaagtcgcggcatcgaacggctgggcagcacccaattcaaaccggtggatatgcgggtgattgtcgccacgcagacgccattgcagaaactggtggacgaagggcgttttcggcgtgacctctacttccggctggatacggtaaaaattcagctgccgacgctgcgttcgcgtacggaaatcattctgccgttattcgagcgctttttgctcgaggcctcggcccgcctgaaacggccggcgccgggcctgtcggccatcattcaggaacaactgctgatgcacggctggcccggcaacattcgtgagttgaaggcggcggccgagcgctgggtgctggggctgtcgccggtgccgatagccagtgatggcgacgtgcaggtaatgggagaggatacgccgctgacgtcgctgaaagtgcgcctgcgccgcattgagcggtttctgattcaggaagcgttgcagcgcaacgaccactgtatcgatacggtggtgaatgaactgggcattcccaaacgcacgctctatcaccgcatcaagctgctgaatgtggcggcgcgcgggctgtaaaaacggcgaagcttaagcttcgccgttttttttttc BBa_M39010_sequence 1 aagcagatcgcattataagctttctgtatggggtgtttctagagaaagaggggacaaactagatggcaacgaatttacgtggcgtaatggctgcactcctgactccttttgaccaacaacaagcactggataaagcgagtctgcgtcgcctggttcagttcaatattcagcagggcatcgacggtttatacgtgggtggttcgaccggcgaggcctttgtacaaagcctttccgagcgtgaacaggtactggaaatcgtcgccgaagaggcgaaaggtaagattaaactcatcgcccacgtcggttgcgtcagcaccgccgaaagccaacaacttgcggcatcggctaaacgttatggcttcgatgccgtctccgccgtcacgccgttctactatcctttcagctttgaagaacactgcgatcactatcgggcaattattgattcggcggatggtttgccgatggtggtgtacaacattccagccctgagtggggtaaaactgaccctggatcagatcaacacacttgttacattgcctggcgtaggtgcgctgaaacagacctctggcgatctctatcagatggagcagatccgtcgtgaacatcctgatcttgtgctctataacggttacgacgaaatcttcgcctctggtctgctggcgggcgctgatggtggtatcggcagtacctacaacatcatgggctggcgctatcaggggatcgttaaggcgctgaaagaaggcgatatccagaccgcgcagaaactgcaaactgaatgcaataaagtcattgatttactgatcaaaacgggcgtattccgcggcctgaaaactgtcctccattatatggatgtcgtttctgtgccgctgtgccgcaaaccgtttggaccggtagatgaaaaatatctgccagaactgaaggcgctggcccagcagttgatgcaagagcgcgggtgaaagcagatcgcattataagctttctgtatggggtgtttctagagaaagaggggacaaactagatgtcgttacttgcacaactggatcaaaaaatcgctgctaacggtggcctgattgtctcctgccagccggttccggacagcccgctcgataaacccgaaatcgtcgccgccatggcattagcggcagaacaggcgggcgcggttgccattcgcattgaaggtgtggcaaatctgcaagccacgcgtgcggtggtgagcgtgccgattattggaattgtgaaacgcgatctggaggattctccggtacgcatcacggcctatattgaagatgttgatgcgctggcgcaggcgggcgcggacattatcgccattgacggcaccgaccgcccgcgtccggtgcctgttgaaacgctgctggcacgtattcaccatcacggtttactggcgatgaccgactgctcaacgccggaagacggcctggcatgccaaaagctgggagccgaaattattggcactacgctttctggctataccacgcctgaaacgccagaagagccggatctggcgctggtgaaaacgttgagcgacgccggatgtcgggtgattgccgaagggcgttacaacacgcctgctcaggcggcggatgcgatgcgccacggcgcgtgggcggtgacggtcggttctgcaatcacgcgtcttgagcacatttgtcagtggtacaacacagcgatgaaaaaggcggtgctatgaaagcagatcgcattataagctttctgtatggggtgtttctagagaaagaggggacaaactagatgaccacactggcgattgatatcggcggtactaaacttgccgccgcgctgattggcgctgacgggcagatccgcgatcgtcgtgaacttcctacgccagccagccagacaccagaagccttgcgtgatgccttatccgcattagtctctccgttgcaagctcatgcgcagcgggttgccatcgcttcgaccgggataatccgtgacggcagcttgctggcgcttaatccgcataatcttggtggattgctacactttccgttagtcaaaacgctggaacaacttaccaatttgccgaccattgccattaacgacgcgcaggccgcagcatgggcggagtttcaggcgctggatggcgatataaccgatatggtctttatcaccgtttccaccggcgttggcggcggtgtagtgagcggctgcaaactgcttaccggccctggcggtctggcggggcatatcgggcatacgcttgccgatccacacggcccagtctgcggctgtggacgcacaggttgcgtggaagcgattgcttctggtcgcggcattgcagcggcagcgcagggggagttggctggcgcggatgcgaaaactattttcacgcgcgccgggcagggtgacgagcaggcgcagcagctgattcaccgctccgcacgtacgcttgcaaggctgatcgctgatattaaagccacaactgattgccagtgcgtggtggtcggtggcagcgttggtctggcagaagggtatctggcgctggtggaaacgtatctggcgcaggagccagcggcatttcatgttgatttactggcggcgcattaccgccatgatgcaggtttacttggggctgcgctgttggcccagggagaaaaattatgaaaacggcgaagcttaagcttcgccgttttttttttc BBa_M39007_sequence 1 tgccactttagtgaagcagatcgcattataagctttctgtatggggtgttgcttaattgatctggtataacaggtataaaggtatatcgtttatcagacaagcatcacttcagaggtattt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z