LOCUS       BBa_M39022              1532 bp    DNA     linear       29-Nov-2024
DEFINITION  Kill Switch
ACCESSION   BBa_M39022
VERSION     BBa_M39022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..87
                     /label=rba
     CDS             88..516
                     /label=Granuylsin
     misc_feature    517..552
                     /label=Terminator
     CDS             63..552
                     /label=BBa_M39020
     misc_feature    561..579
                     /label=TetR 1
     misc_feature    561..614
                     /label=BBa_R0040
     promoter        580..585
                     /label=-35
     misc_feature    586..604
                     /label=TetR 2
     promoter        603..608
                     /label=-10
     promoter        561..614
                     /label=BBa_R0040
     RBS             623..647
                     /label=rbs
     CDS             648..1496
                     /label=DNase1
     misc_feature    1497..1532
                     /label=Terminator
     CDS             623..1532
                     /label=BBa_M39021
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtctagaga aagaggggac aaactagatg gcaacatggg ctctgctgct gctggctgct
      121 atgctgctgg gtaatcctgg tctggttttc tctcgtctgt ctcctgagta ttatgatctg
      181 gcccgtgctc acctgcgtga tgaggaaaaa tcttgtcctt gcctggcaca ggaaggtcca
      241 cagggcgacc tgctgacaaa aacacaggaa ctgggccgtg attatcgtac atgtctgacc
      301 gttcagaaac tgaaaaaaat ggtggataaa ccgacacaac gttccgttag caatgccgct
      361 acacgtgtct gtcgtaccgg tcgttctcgt tggcgtgacg tttgccgtaa ctttatgcgt
      421 cgctatcagt ctcgtgttac acaaggcctg gttgctggtg aaacagcaca acagtgtgag
      481 gatctgcgtc tgtgtccatc aactggtcct ctgtagaaac ggcgaagctt aagcttcgcc
      541 gttttttttt tctactagag tccctatcag tgatagagat tgacatccct atcagtgata
      601 gagatactga gcactactag agtctagaga aagaggggac aaactagatg aggggcatga
      661 agctgctggg ggcgctgctg gcactggcgg ccctactgca gggggccgtg tccctgaaga
      721 tcgcagcctt caacatccag acatttgggg agaccaagat gtccaatgcc accctcgtca
      781 gctacattgt gcagatcctg agccgctatg acatcgccct ggtccaggag gtcagagaca
      841 gccacctgac tgccgtgggg aagctgctgg acaacctcaa tcaggatgca ccagacacct
      901 atcactacgt ggtcagtgag ccactgggac ggaacagcta taaggagcgc tacctgttcg
      961 tgtacaggcc tgaccaggtg tctgcggtgg acagctacta ctacgatgat ggctgcgagc
     1021 cctgcgggaa cgacaccttc aaccgagagc cagccattgt caggttcttc tcccggttca
     1081 cagaggtcag ggagtttgcc attgttcccc tgcatgcggc cccgggggac gcagtagccg
     1141 agatcgacgc tctctatgac gtctacctgg atgtccaaga gaaatggggc ttggaggacg
     1201 tcatgttgat gggcgacttc aatgcgggct gcagctatgt gagaccctcc cagtggtcat
     1261 ccatccgcct gtggacaagc cccaccttcc agtggctgat ccccgacagc gctgacacca
     1321 cagctacacc cacgcactgt gcctatgaca ggatcgtggt tgcagggatg ctgctccgag
     1381 gcgccgttgt tcccgactcg gctcttccct ttaacttcca ggctgcctat ggcctgagtg
     1441 accaactggc ccaagccatc agtgaccact atccagtgga ggtgatgctg aagtgaaaac
     1501 ggcgaagctt aagcttcgcc gttttttttt tc
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