LOCUS BBa_M39023 10861 bp DNA linear 18-Feb-2025 DEFINITION AND gate structure for regulation of E.coli in CF mucus ACCESSION BBa_M39023 VERSION BBa_M39023.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..68 /label=rbs CDS 69..860 /label=NanR misc_feature 861..896 /label=Termintor CDS 44..896 /label=BBa_M39008 misc_feature 968..973 /label=NanR Binding Site misc_feature 977..982 /label=NanR Binding Site misc_feature 982..982 /label=+1 Site misc_feature 985..990 /label=NanR Binding Site promoter 905..1025 /label=BBa_M39007 RBS 1032..1056 /label=rbs CDS 1057..2522 /label=NanT CDS 1032..2522 /label=BBa_M39009 RBS 2567..2592 /label=rbs CDS 2593..3487 /label=NanA RBS 3524..3548 /label=rbs CDS 3549..4239 /label=NanE RBS 4276..4300 /label=rbs CDS 4301..5176 /label=NanK misc_feature 5177..5212 /label=Terminator CDS 2531..5212 /label=BBa_M39010 misc_feature 5284..5289 /label=NanR Binding Site misc_feature 5293..5298 /label=NanR Binding Site misc_feature 5298..5298 /label=+1 Site misc_feature 5301..5306 /label=NanR Binding Site promoter 5221..5341 /label=BBa_M39007 RBS 5350..5374 /label=rbs CDS 5375..6358 /label=HrpS misc_feature 6359..6394 /label=Terminator CDS 5350..6394 /label=BBa_M39011 misc_feature 1..6394 /label=BBa_M39012 promoter 6403..6437 /label=BBa_J23100 RBS 6446..6470 /label=rbs CDS 6471..7190 /label=LasR misc_feature 7191..7226 /label=Terminator CDS 6446..7226 /label=BBa_M39013 misc_feature 7308..7328 /label=LasR Binding Site promoter 7235..7360 /label=BBa_M39014 RBS 7369..7393 /label=rbs CDS 7394..8338 /label=HrpR misc_feature 8339..8374 /label=misc CDS 7369..8374 /label=BBa_M39015 misc_feature 6403..8374 /label=BBa_M39016 misc_feature 8443..8503 /label=Upstream Activation Sequence misc_feature 8527..8548 /label=Integration Host Factor promoter 8383..8592 /label=BBa_M39018 RBS 8601..8625 /label=rbs CDS 8626..9285 /label=TetR misc_feature 9286..9321 /label=Terminator CDS 8601..9321 /label=BBa_M39017 misc_feature 8383..9321 /label=BBa_M39019 misc_feature 9330..9348 /label=TetR 1 misc_feature 9330..9383 /label=BBa_R0040 promoter 9349..9354 /label=-35 misc_feature 9355..9373 /label=TetR 2 promoter 9372..9377 /label=-10 promoter 9330..9383 /label=BBa_R0040 RBS 9392..9416 /label=rba CDS 9417..9845 /label=Granuylsin misc_feature 9846..9881 /label=Terminator CDS 9392..9881 /label=BBa_M39020 misc_feature 9890..9908 /label=TetR 1 misc_feature 9890..9943 /label=BBa_R0040 promoter 9909..9914 /label=-35 misc_feature 9915..9933 /label=TetR 2 promoter 9932..9937 /label=-10 promoter 9890..9943 /label=BBa_R0040 RBS 9952..9976 /label=rbs CDS 9977..10825 /label=DNase1 misc_feature 10826..10861 /label=Terminator CDS 9952..10861 /label=BBa_M39021 misc_feature 9330..10861 /label=BBa_M39022 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtctagag aaagagggga 61 caaactagat gggccttatg aacgcatttg attcgcaaac cgaagattct tcacctgcaa 121 ttggtcgcaa cttgcgtagc cgcccgctgg cgcgtaaaaa actctccgaa atggtggaag 181 aagagctgga acagatgatc cgccgtcgtg aatttggcga aggtgaacaa ttaccgtctg 241 aacgcgaact gatggcgttc tttaacgtcg ggcgtccttc ggtgcgtgaa gcgctggcag 301 cgttaaaacg caaaggtctg gtgcaaataa acaacggcga acgcgctcgc gtctcgcgtc 361 cttctgcgga cactatcatc ggtgagcttt ccggcatggc gaaagatttc ctttctcatc 421 ccggtgggat tgcccatttc gaacaattac gtctgttctt tgaatccagt ctggtgcgct 481 atgcggctga acatgccacc gatgagcaaa tcgatttgct ggcaaaagca ctggaaatca 541 acagtcagtc gctggataac aacgcggcat tcattcgttc agacgttgat ttccaccgcg 601 tgctggcgga gatccccggt aacccaatct tcatggcgat ccacgttgcc ctgctcgact 661 ggcttattgc cgcacgccca acggttaccg atcaggcact gcacgaacat aacaacgtta 721 gttatcaaca gcatattgcg atcgttgatg cgatccgccg tcatgatcct gacgaagccg 781 atcgtgcgtt gcaatcgcat ctcaacagcg tctctgctac ctggcacgct ttcggtcaga 841 ccaccaacaa aaagaaataa aaacggcgaa gcttaagctt cgccgttttt tttttctact 901 agagtgccac tttagtgaag cagatcgcat tataagcttt ctgtatgggg tgttgcttaa 961 ttgatctggt ataacaggta taaaggtata tcgtttatca gacaagcatc acttcagagg 1021 tattttacta gatgagtact acaacccaga atatcccgtg gtatcgccat ctcaaccgtg 1081 cacaatggcg cgcattttcc gctgcctggt tgggatatct gcttgacggt tttgatttcg 1141 ttttaatcgc cctggtactc accgaagtac aaggtgaatt cgggctgacg acggtgcagg 1201 cggcaagtct gatctctgca gcctttatct ctcgctggtt cggcggcctg atgctcggcg 1261 ctatgggtga ccgctacggg cgtcgtctgg caatggtcac cagcatcgtt ctcttctcgg 1321 ccgggacgct ggcctgcggc tttgcgccag gctacatcac catgtttatc gctcgtctgg 1381 tcatcggcat ggggatggcg ggtgaatacg gttccagcgc cacctatgtc attgaaagct 1441 ggccaaaaca tctgcgtaac aaagccagtg gttttttgat ttcaggcttc tctgtggggg 1501 ccgtcgttgc cgctcaggtc tatagcctgg tggttccggt ctggggctgg cgtgcgctgt 1561 tctttatcgg cattttgcca atcatctttg ctctctggct gcgtaaaaac atcccggaag 1621 cggaagactg gaaagagaaa cacgcaggta aagcaccagt acgcacaatg gtggatattc 1681 tctaccgtgg tgaacatcgc attgccaata tcgtaatgac actggcggcg gctactgcgc 1741 tgtggttctg cttcgccggt aacctgcaaa atgccgcgat cgtcgctgtt cttgggctgt 1801 tatgcgccgc aatctttatc agctttatgg tgcagagtgc aggcaaacgc tggccaacgg 1861 gcgtaatgct gatggtggtc gtgttgtttg ctttcctcta ctcatggccg attcaggcgc 1921 tgctgccaac gtatctgaaa accgatctgg cttataaccc gcatactgta gccaatgtgc 1981 tgttctttag tggctttggc gcggcggtgg gatgctgcgt aggtggcttc ctcggtgact 2041 ggctgggaac ccgcaaagcg tacgtttgta gcctgctggc ctcgcagctg ctgattattc 2101 cggtatttgc gattggcggc gcaaacgtct gggtgctcgg tctgttactg ttcttccagc 2161 aaatgcttgg acaagggatc gccgggatct taccaaaact gattggcggt tatttcgata 2221 ccgaccagcg tgcagcgggc ctgggcttta cctacaacgt tggcgcattg ggcggtgcac 2281 tggccccaat catcggcgcg ttgatcgctc aacgtctgga tctgggtact gcgctggcat 2341 cgctctcgtt cagtctgacg ttcgtggtga tcctgctgat tgggctggat atgccttctc 2401 gcgttcagcg ttggttgcgc ccggaagcgt tgcgtactca tgacgctatc gacggtaaac 2461 cattcagcgg tgccgtgccg tttggcagcg ccaaaaacga tttagtcaaa accaaaagtt 2521 aatactagag aagcagatcg cattataagc tttctgtatg gggtgtttct agagaaagag 2581 gggacaaact agatggcaac gaatttacgt ggcgtaatgg ctgcactcct gactcctttt 2641 gaccaacaac aagcactgga taaagcgagt ctgcgtcgcc tggttcagtt caatattcag 2701 cagggcatcg acggtttata cgtgggtggt tcgaccggcg aggcctttgt acaaagcctt 2761 tccgagcgtg aacaggtact ggaaatcgtc gccgaagagg cgaaaggtaa gattaaactc 2821 atcgcccacg tcggttgcgt cagcaccgcc gaaagccaac aacttgcggc atcggctaaa 2881 cgttatggct tcgatgccgt ctccgccgtc acgccgttct actatccttt cagctttgaa 2941 gaacactgcg atcactatcg ggcaattatt gattcggcgg atggtttgcc gatggtggtg 3001 tacaacattc cagccctgag tggggtaaaa ctgaccctgg atcagatcaa cacacttgtt 3061 acattgcctg gcgtaggtgc gctgaaacag acctctggcg atctctatca gatggagcag 3121 atccgtcgtg aacatcctga tcttgtgctc tataacggtt acgacgaaat cttcgcctct 3181 ggtctgctgg cgggcgctga tggtggtatc ggcagtacct acaacatcat gggctggcgc 3241 tatcagggga tcgttaaggc gctgaaagaa ggcgatatcc agaccgcgca gaaactgcaa 3301 actgaatgca ataaagtcat tgatttactg atcaaaacgg gcgtattccg cggcctgaaa 3361 actgtcctcc attatatgga tgtcgtttct gtgccgctgt gccgcaaacc gtttggaccg 3421 gtagatgaaa aatatctgcc agaactgaag gcgctggccc agcagttgat gcaagagcgc 3481 gggtgaaagc agatcgcatt ataagctttc tgtatggggt gtttctagag aaagagggga 3541 caaactagat gtcgttactt gcacaactgg atcaaaaaat cgctgctaac ggtggcctga 3601 ttgtctcctg ccagccggtt ccggacagcc cgctcgataa acccgaaatc gtcgccgcca 3661 tggcattagc ggcagaacag gcgggcgcgg ttgccattcg cattgaaggt gtggcaaatc 3721 tgcaagccac gcgtgcggtg gtgagcgtgc cgattattgg aattgtgaaa cgcgatctgg 3781 aggattctcc ggtacgcatc acggcctata ttgaagatgt tgatgcgctg gcgcaggcgg 3841 gcgcggacat tatcgccatt gacggcaccg accgcccgcg tccggtgcct gttgaaacgc 3901 tgctggcacg tattcaccat cacggtttac tggcgatgac cgactgctca acgccggaag 3961 acggcctggc atgccaaaag ctgggagccg aaattattgg cactacgctt tctggctata 4021 ccacgcctga aacgccagaa gagccggatc tggcgctggt gaaaacgttg agcgacgccg 4081 gatgtcgggt gattgccgaa gggcgttaca acacgcctgc tcaggcggcg gatgcgatgc 4141 gccacggcgc gtgggcggtg acggtcggtt ctgcaatcac gcgtcttgag cacatttgtc 4201 agtggtacaa cacagcgatg aaaaaggcgg tgctatgaaa gcagatcgca ttataagctt 4261 tctgtatggg gtgtttctag agaaagaggg gacaaactag atgaccacac tggcgattga 4321 tatcggcggt actaaacttg ccgccgcgct gattggcgct gacgggcaga tccgcgatcg 4381 tcgtgaactt cctacgccag ccagccagac accagaagcc ttgcgtgatg ccttatccgc 4441 attagtctct ccgttgcaag ctcatgcgca gcgggttgcc atcgcttcga ccgggataat 4501 ccgtgacggc agcttgctgg cgcttaatcc gcataatctt ggtggattgc tacactttcc 4561 gttagtcaaa acgctggaac aacttaccaa tttgccgacc attgccatta acgacgcgca 4621 ggccgcagca tgggcggagt ttcaggcgct ggatggcgat ataaccgata tggtctttat 4681 caccgtttcc accggcgttg gcggcggtgt agtgagcggc tgcaaactgc ttaccggccc 4741 tggcggtctg gcggggcata tcgggcatac gcttgccgat ccacacggcc cagtctgcgg 4801 ctgtggacgc acaggttgcg tggaagcgat tgcttctggt cgcggcattg cagcggcagc 4861 gcagggggag ttggctggcg cggatgcgaa aactattttc acgcgcgccg ggcagggtga 4921 cgagcaggcg cagcagctga ttcaccgctc cgcacgtacg cttgcaaggc tgatcgctga 4981 tattaaagcc acaactgatt gccagtgcgt ggtggtcggt ggcagcgttg gtctggcaga 5041 agggtatctg gcgctggtgg aaacgtatct ggcgcaggag ccagcggcat ttcatgttga 5101 tttactggcg gcgcattacc gccatgatgc aggtttactt ggggctgcgc tgttggccca 5161 gggagaaaaa ttatgaaaac ggcgaagctt aagcttcgcc gttttttttt tctactagag 5221 tgccacttta gtgaagcaga tcgcattata agctttctgt atggggtgtt gcttaattga 5281 tctggtataa caggtataaa ggtatatcgt ttatcagaca agcatcactt cagaggtatt 5341 ttactagagt ctagagaaag aggggacaaa ctagatgacg ataaggaata gcaatgaatc 5401 cgtgcagtca cttcattgtg cctcccatga gtcctcgtta acgcatccgt cggaagatat 5461 acacggatcg ctatcttctc tcatccacac tatcgcgccc ctcaacgttg atatcgtact 5521 ggaaggcgaa accggcaccg gcaaagacac gctggcgcgc cgtattcatc agctttccgg 5581 ctgcgccggc ccgttggtgg cggtgaactg cgccgccgtg ccggaaacgc tggcggaaag 5641 cgagttattc ggggtggtgt ccggcgctta caccggcgcc agccggtccc gcgccggata 5701 ccttgaaacc gcggacaaag gcattctgtt tctggatgag atcgacagta tgccgctgac 5761 gctgcaggcc aaaatgttac gggttctgga aagtcgcggc atcgaacggc tgggcagcac 5821 ccaattcaaa ccggtggata tgcgggtgat tgtcgccacg cagacgccat tgcagaaact 5881 ggtggacgaa gggcgttttc ggcgtgacct ctacttccgg ctggatacgg taaaaattca 5941 gctgccgacg ctgcgttcgc gtacggaaat cattctgccg ttattcgagc gctttttgct 6001 cgaggcctcg gcccgcctga aacggccggc gccgggcctg tcggccatca ttcaggaaca 6061 actgctgatg cacggctggc ccggcaacat tcgtgagttg aaggcggcgg ccgagcgctg 6121 ggtgctgggg ctgtcgccgg tgccgatagc cagtgatggc gacgtgcagg taatgggaga 6181 ggatacgccg ctgacgtcgc tgaaagtgcg cctgcgccgc attgagcggt ttctgattca 6241 ggaagcgttg cagcgcaacg accactgtat cgatacggtg gtgaatgaac tgggcattcc 6301 caaacgcacg ctctatcacc gcatcaagct gctgaatgtg gcggcgcgcg ggctgtaaaa 6361 acggcgaagc ttaagcttcg ccgttttttt tttctactag agttgacggc tagctcagtc 6421 ctaggtacag tgctagctac tagagtctag agaaagaggg gacaaactag atggccttgg 6481 ttgacggttt tcttgagctg gaacgctcaa gtggaaaatt ggagtggagc gccatcctgc 6541 agaagatggc gagcgacctt ggattctcga agatcctgtt cggcctgttg cctaaggaca 6601 gccaggacta cgagaacgcc ttcatcgtcg gcaactaccc ggccgcctgg cgcgagcatt 6661 acgaccgggc tggctacgcg cgggtcgacc cgacggtcag tcactgtacc cagagcgtac 6721 tgccgatttt ctgggaaccg tccatctacc agacgcgaaa gcagcacgag ttcttcgagg 6781 aagcctcggc cgccggcctg gtgtatgggc tgaccatgcc gctgcatggt gctcgcggcg 6841 aactcggcgc gctgagcctc agcgtggaag cggaaaaccg ggccgaggcc aaccgtttca 6901 tggagtcggt cctgccgacc ctgtggatgc tcaaggacta cgcactgcag agcggtgccg 6961 gactggcctt cgaacatccg gtcagcaaac cggtggttct gaccagccgg gagaaggaag 7021 tgttgcagtg gtgcgccatc ggcaagacca gttgggagat atcggttatc tgcaactgct 7081 cggaagccaa tgtgaacttc catatgggaa atattcggcg gaagttcggt gtgacctccc 7141 gccgcgtagc ggccattatg gccgttaatt tgggtcttat tactctctga aaacggcgaa 7201 gcttaagctt cgccgttttt tttttctact agagtgttct cgtgtgaagc cattgctctg 7261 atcttttgga cgtttcttcg agcctagcaa gggtccgggt tcaccgaaat ctatctcatt 7321 tgctagttat aaaattatga aatttgtata aattcttcag tactagagtc tagagaaaga 7381 ggggacaaac tagatgagca cagacattga taacgacgtt ctaacgtgtt gcaacgtaac 7441 tgcattatcg gccggtcatc aaattgtaat gaacagcgtg ctaatggata tggacttgct 7501 tctttgcgga gaaaccggca ccggcaaaga taccctggcc agccggattc acgagctgtc 7561 gagcaggacc gggccttttg tcggcatgaa ctgtgcggcc atccccgaat cgttggcaga 7621 gagccagttg ttcggcgtgg tcaatggtgc gttcaccggc gtgtgtcggg ctcgcgaggg 7681 ctacatcgaa gcctccagcg gcggcaccct ctatctggat gaaatcgaca gcatgccgtt 7741 gagccttcag gccaagctct tgcgggtgct ggaaagcagg ggcgtcgagc gactcggctc 7801 gaccgacttc ataccactgg atctgcgagt gattgcttcg gcccagcgtc cgctggatga 7861 actggtggaa caaggacttt ttcgtcgtga cctgtttttc aggctcaatg tgctgacact 7921 gcagttgccg gccttgcgca agcgtcgcga gcagattttg ccattgttcg accagttcac 7981 ccaggacgtc gccgcagaat ccggccgttc cgttccaacg ctcgacaatc gacgtgtaca 8041 gatcctgttg agccatgact ggcccggcaa cgtgcgcgaa ctgaagtcgg ctgccaagcg 8101 atttgtcttg gggttgccgt tgctgggcgc tgagccggtc gaagcgcgtg acccggtaac 8161 agggctgcgc atgcagatgc gcgtcatcga gaaaatgctc attcaggatg ccttgaagcg 8221 acatcggcac aatttcgacg ctgtgctcga ggagctcgag ctgccccggc gcaccctcta 8281 ccaccgcatg aaagagctgg gtgttgcttc gcatatcgac ctggtagccg agtcctgaaa 8341 acggcgaagc ttaagcttcg ccgttttttt tttctactag aggccggatt atgtccgctg 8401 agtgggtcac ggtcccggat cagttccctt gcgaagctga ccgatgtttt tgatgccaaa 8461 agctgttgtg gcaaaaaacg gtttgcgcaa agttttgtat tacaaagaat ttcacatttt 8521 aaatatatct ttataaatca atcagttatt tctattttta agctggcatg gttatcgcta 8581 tagggcttgt actactagag tctagagaaa gaggggacaa actagatgtc cagattagat 8641 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta 8701 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta 8761 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact 8821 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt 8881 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct 8941 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt 9001 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta 9061 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt 9121 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc 9181 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt 9241 gggtccgctg caaacgacga aaactacgct ttagtagctt aataaaaacg gcgaagctta 9301 agcttcgccg tttttttttt ctactagagt ccctatcagt gatagagatt gacatcccta 9361 tcagtgatag agatactgag cactactaga gtctagagaa agaggggaca aactagatgg 9421 caacatgggc tctgctgctg ctggctgcta tgctgctggg taatcctggt ctggttttct 9481 ctcgtctgtc tcctgagtat tatgatctgg cccgtgctca cctgcgtgat gaggaaaaat 9541 cttgtccttg cctggcacag gaaggtccac agggcgacct gctgacaaaa acacaggaac 9601 tgggccgtga ttatcgtaca tgtctgaccg ttcagaaact gaaaaaaatg gtggataaac 9661 cgacacaacg ttccgttagc aatgccgcta cacgtgtctg tcgtaccggt cgttctcgtt 9721 ggcgtgacgt ttgccgtaac tttatgcgtc gctatcagtc tcgtgttaca caaggcctgg 9781 ttgctggtga aacagcacaa cagtgtgagg atctgcgtct gtgtccatca actggtcctc 9841 tgtagaaacg gcgaagctta agcttcgccg tttttttttt ctactagagt ccctatcagt 9901 gatagagatt gacatcccta tcagtgatag agatactgag cactactaga gtctagagaa 9961 agaggggaca aactagatga ggggcatgaa gctgctgggg gcgctgctgg cactggcggc 10021 cctactgcag ggggccgtgt ccctgaagat cgcagccttc aacatccaga catttgggga 10081 gaccaagatg tccaatgcca ccctcgtcag ctacattgtg cagatcctga gccgctatga 10141 catcgccctg gtccaggagg tcagagacag ccacctgact gccgtgggga agctgctgga 10201 caacctcaat caggatgcac cagacaccta tcactacgtg gtcagtgagc cactgggacg 10261 gaacagctat aaggagcgct acctgttcgt gtacaggcct gaccaggtgt ctgcggtgga 10321 cagctactac tacgatgatg gctgcgagcc ctgcgggaac gacaccttca accgagagcc 10381 agccattgtc aggttcttct cccggttcac agaggtcagg gagtttgcca ttgttcccct 10441 gcatgcggcc ccgggggacg cagtagccga gatcgacgct ctctatgacg tctacctgga 10501 tgtccaagag aaatggggct tggaggacgt catgttgatg ggcgacttca atgcgggctg 10561 cagctatgtg agaccctccc agtggtcatc catccgcctg tggacaagcc ccaccttcca 10621 gtggctgatc cccgacagcg ctgacaccac agctacaccc acgcactgtg cctatgacag 10681 gatcgtggtt gcagggatgc tgctccgagg cgccgttgtt cccgactcgg ctcttccctt 10741 taacttccag gctgcctatg gcctgagtga ccaactggcc caagccatca gtgaccacta 10801 tccagtggag gtgatgctga agtgaaaacg gcgaagctta agcttcgccg tttttttttt 10861 c //