AND gate structure for regulation of E.coli in CF mucus
This device will have the cells live in the presence of LasL and Sialic acid
This design will produce one of two necessary transcription factors for the HrpL AND gate. This will produce it in the presence of Sialic Acid
This device will detect the presence of LasL which is a signaling molecule used by P. aeruginosa. This is tied to make HrpR which is needed in the HrpL AND gate.
This device, in the presence of HrpS and HrpR, will produce the regulator TetR
In the absence of tetR, this device will make granulysin and DNase1 which will kill the cell.
we needed to have a way to make sure both the membrane and genome of the cell was destroyed. These enzymes seemed like the best idea.
This is the promoter sequence for the NanR transcription factor in the Nan Operon. This Operon is for Sialic Acid Degradation. Promoter is for E.Coli
This codes for the NanR Regulator. This is the the repressor for the Nan Operon and is turned off by the presence of sialic acid.
This allows the transport of Sialic Acid into the cell
These are the enzymes needed for Sialic Acid breakdown in E.Coli
Since these are all on an operon, we made sure there was sufficient space and an RBS in-between each coding region
This codes for one of the two necessary genes (HrpS and HrpR) that will be needed to activate the HrpL AND gate
Coding region for the LasL protein which is used in P. aeruginosa quorum sensing
the promoter region for LasL regulator protein
Codes for HrpR. This is one of two proteins (HrpS and HrpR) needed for the HrpL AND gate.
Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
Sequence for pTet inverting regulator driven by the TetR protein.
BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
This is the coding region for the TetR repressor.
This promoter sequence requires both HrpR and HrpS to be expressed for itself to be active. This functions as a digital AND gate
Coding region for enzyme that degrades membrane
Coding region for DNase1, enzyme with unspecific degradation of ds DNA
Conversion of the iGEM parts registry to SBOL2.1